2026 QUESTIONS WITH ANSWERS
GRADED A+
◍ Wiener terminology.
Answer: 8 alleles exist at the single Rh gene locusR0, R1, R2, Rz, r, r', r",
ry•Each gene encodes an agglutinogen (made of factors) that correlates with
Rh antigensExample 1: R1 Rh1(Rh0, rh', hr") D, C, e
◍ Cold alloantibodies.
Answer: Antibodies specific for human RBC antigens that react at room
temperature or below
◍ Potentiators.
Answer: Reagents that enhance the detection of IgG antibodies by
increasing their reactivity
◍ Punnett square.
Answer: used to predict the probability of an offspring's genotype
◍ polymorphic.
Answer: Multiple alleles at a single locus
◍ heterozygous.
Answer: A person who inherits different alleles-AO, AB, MN (M+ N+)
◍ Common genotypes by race.
Answer: Caucasians: R1, r, R2, R0-Blacks: R0, r, R1, R2-r', r", Rz, and ry
are rarely encountered
◍ Reagents must meet minimum standards before being licensed.
Answer: -Specificity: recognition of the antigenic determinant and its
corresponding antibody-Potency: strength of the reaction
,◍ universal donor (plasma).
Answer: Group AB:
◍ ABO antibodies are?.
Answer: non-RBC stimulated, or naturally occurring-Antibody production is
unrelated to an RBC antigen-Results from exposure to A and B like antigens
in the environment (e.g., normal bacterial flora)
◍ D antigen typing procedure.
Answer: Commercial anti-D is combined with the patient and donor RBCs.
Agglutination indicates the presence of the D antigen; no agglutination
indicates the absence of the D antigen. A negative reagent control ensures
that a false-positive result has not occurred.
◍ Rare subgroups of the A type include.
Answer: Aint (intermediate), A3, Ax, Am, Aend, Ael, Abantu
◍ Polyspecific AHG aka antigloublin test.
Answer: Contains both anti-IgG and anti-C3d antibodies •Derived from
polyclonal or monoclonal sources •Agglutination indicates that IgG or
complement is coating the RBCs •If positive, a differential DAT is
performed
◍ Sources of antibody.
Answer: •Reagent antisera-Commercially prepared-Known source of
antibody •Patient or donor serum or plasma-Usually unknown-Serum or
plasma is tested with commercial RBCs to determine identity of antibody or
antibodies
◍ RBCs that test positive only by the indirect antiglobulin test (IAT) are
called.
Answer: weak D antigens
◍ The FDA's criteria can be found in the Code of _____________.
Answer: Federal Regulations
◍ Compare and contrast polyclonal and monoclonal antibodies(polyclonal
, antibodies).
Answer: Commercial Antibody Reagents-Made from several different
clones of B cells that secrete antibodies of different specificities-Recognize
multiple epitopes-Example: antihuman globulin (AHG)
◍ Anti A1 antibody.
Answer: Produced by subgroups of the A type •Has specificity to the A1
antigen but will not agglutinate A2 cells •Not clinically significant •May
cause incompatible crossmatches on IS testing++++
◍ Hardy-Weinberg formula.
Answer: calculates a determination of the gene frequencies that produced a
trait
◍ Resolution of weak/missing antigens.
Answer: It is important to check the patient's diagnosis and transfusion
history-Repeat with anti-A,B to enhance subgroup
◍ The oligosaccharide chain is.
Answer: a basic precursor structure for several RBC antigens, including A,
B, and H •Attached to a protein or lipid carrier molecule
◍ Titers.
Answer: maximum levels by 5 to 10 years of age and decrease as an
individual grows older
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◍ The 2 main subgroups of A.
Answer: A1 , A2Both react strongly with reagent anti-A (3+ to 4+)
◍ To determine genotype or phenotype occurrence, two formulas are used:.
Answer: Phenotype calculationHardy-Weinberg formula