Growth Requirements of Bacteria
1)
2)
3) - ANSWER 1) Nutrients
2) Temperature
3) pH
Columbia CNA Agar
Selective:
Differential:
Enriched:
Special cultures: - ANSWER Selective: Yes
Differential: Yes
Enriched: Yes
Special cultures: Gram-Positive Microbes
Chocolate Agar
Selective:
Differential:
Enriched: - ANSWER Selective: No
Differential: No
1
, Enriched: Yes
NUTRIENTS: Microbial Categories (Carbon and Energy):
1)
2)
3)
4) - ANSWER 1) Photoautotrophs- Light energy and CO2
2) Photoheterotrophs- Light energy and organic compounds
3) Chemoautotrophs- Chemical energy and CO2
4) Chemoheterotrophs- Chemical energy and organic compounds
NUTRIENTS: Microbial Categories (Oxygen):
1)
2)
3) - ANSWER 1) Obligate Aerobes: Require O2
2) Obligate Anaerobes: Killed by O2
3) Facultative Anaerobes: Grow better with O2, but can live without it
Other Nutrients (Not for Classification):
1)
2)
3) - ANSWER 1) Nitrogen
2) Trace elements
3) Growth factors
TEMPERATURE: Microbial Categories:
1)
2
1)
2)
3) - ANSWER 1) Nutrients
2) Temperature
3) pH
Columbia CNA Agar
Selective:
Differential:
Enriched:
Special cultures: - ANSWER Selective: Yes
Differential: Yes
Enriched: Yes
Special cultures: Gram-Positive Microbes
Chocolate Agar
Selective:
Differential:
Enriched: - ANSWER Selective: No
Differential: No
1
, Enriched: Yes
NUTRIENTS: Microbial Categories (Carbon and Energy):
1)
2)
3)
4) - ANSWER 1) Photoautotrophs- Light energy and CO2
2) Photoheterotrophs- Light energy and organic compounds
3) Chemoautotrophs- Chemical energy and CO2
4) Chemoheterotrophs- Chemical energy and organic compounds
NUTRIENTS: Microbial Categories (Oxygen):
1)
2)
3) - ANSWER 1) Obligate Aerobes: Require O2
2) Obligate Anaerobes: Killed by O2
3) Facultative Anaerobes: Grow better with O2, but can live without it
Other Nutrients (Not for Classification):
1)
2)
3) - ANSWER 1) Nitrogen
2) Trace elements
3) Growth factors
TEMPERATURE: Microbial Categories:
1)
2
