A LEVEL BIOLOGY B ADVANCCING IN
BIOLOGY H422/03 PRACTICAL SKILLS IN
BIOLOGY ACTUAL 2025 PAPER MERGED
WITH MARK SCHEME
vincent marekia
[COMPANY NAME] [Company address]
, 1
*1848154248*
INSTRUCTIONS
• Use black ink. You can use an HB pencil, but only for graphs and diagrams.
• Write your answer to each question in the space provided. If you need extra space use
the lined pages at the end of this booklet. The question numbers must be clearly shown.
• Answer all the questions.
• Where appropriate, your answer should be supported with working. Marks might be
given for using a correct method, even if your answer is wrong.
INFORMATION
• The total mark for this paper is 60.
• The marks for each question are shown in brackets [ ].
• Quality of extended response will be assessed in questions marked with an asterisk (*).
• This document has 20 pages.
ADVICE
• Read each question carefully before you start your answer.
© OCR 2025 [601/4720/9] DC (DE/JG) 346850/3
OCR is an exempt Charity
Turn over
1 Two functions of the roots of plants are to take up water from the soil and to store
starch.
© OCR 2025
, 2
(a) The photomicrograph shows a transverse section of a root in a monocotyledonous
plant.
A
C
B
× 20
(i) The cells labelled A and B are two different types of cell in the vascular tissue of the
root.
Identify the types of cell labelled A and B.
A ............................................................................................................................................
...........
B ............................................................................................................................................
...........
(ii) The diameter of one region of the root is represented by the line C in the image.
The magnification used to produce the image was × 20.
Calculate the actual diameter of C.
Give your answer in mm.
Diameter = ................................................... mm
(iii) One risk when dissecting plant tissues, such as roots, is the potential for injury
when using sharp instruments.
State one other health and safety risk that should be considered before dissecting plan
tissues.
© OCR 2025 Turn over
, 3
...............................................................................................................................................
...........
...............................................................................................................................................
...... [1]
(b) A scientist investigates how the rate of water uptake by roots is affected by the
presence of salt (NaCl ) in the surrounding soil.
This is the method they use:
• Grow 110 tomato plants in pots of soil in a greenhouse.
• Add 50 mmol dm–3 salt solution to the soil of 55 plants, every day.
• The other 55 plants are a control group.
• Calculate the rate of water uptake per day (dm3 d–1) by the roots of each plant.
The results of the investigation are shown in the graph.
Mean
rate of water uptake
(dm3 d–1)
Time (days)
© OCR 2025
BIOLOGY H422/03 PRACTICAL SKILLS IN
BIOLOGY ACTUAL 2025 PAPER MERGED
WITH MARK SCHEME
vincent marekia
[COMPANY NAME] [Company address]
, 1
*1848154248*
INSTRUCTIONS
• Use black ink. You can use an HB pencil, but only for graphs and diagrams.
• Write your answer to each question in the space provided. If you need extra space use
the lined pages at the end of this booklet. The question numbers must be clearly shown.
• Answer all the questions.
• Where appropriate, your answer should be supported with working. Marks might be
given for using a correct method, even if your answer is wrong.
INFORMATION
• The total mark for this paper is 60.
• The marks for each question are shown in brackets [ ].
• Quality of extended response will be assessed in questions marked with an asterisk (*).
• This document has 20 pages.
ADVICE
• Read each question carefully before you start your answer.
© OCR 2025 [601/4720/9] DC (DE/JG) 346850/3
OCR is an exempt Charity
Turn over
1 Two functions of the roots of plants are to take up water from the soil and to store
starch.
© OCR 2025
, 2
(a) The photomicrograph shows a transverse section of a root in a monocotyledonous
plant.
A
C
B
× 20
(i) The cells labelled A and B are two different types of cell in the vascular tissue of the
root.
Identify the types of cell labelled A and B.
A ............................................................................................................................................
...........
B ............................................................................................................................................
...........
(ii) The diameter of one region of the root is represented by the line C in the image.
The magnification used to produce the image was × 20.
Calculate the actual diameter of C.
Give your answer in mm.
Diameter = ................................................... mm
(iii) One risk when dissecting plant tissues, such as roots, is the potential for injury
when using sharp instruments.
State one other health and safety risk that should be considered before dissecting plan
tissues.
© OCR 2025 Turn over
, 3
...............................................................................................................................................
...........
...............................................................................................................................................
...... [1]
(b) A scientist investigates how the rate of water uptake by roots is affected by the
presence of salt (NaCl ) in the surrounding soil.
This is the method they use:
• Grow 110 tomato plants in pots of soil in a greenhouse.
• Add 50 mmol dm–3 salt solution to the soil of 55 plants, every day.
• The other 55 plants are a control group.
• Calculate the rate of water uptake per day (dm3 d–1) by the roots of each plant.
The results of the investigation are shown in the graph.
Mean
rate of water uptake
(dm3 d–1)
Time (days)
© OCR 2025
