Lab 7 Microbial Genetics & Genetic Engineering BIO250L”
Student Name: Jennifer Baeza
Access Code (located on the underside of the lid of your lab kit): ACOF5124A
“Pre-Lab QuesCons”
1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which are
pyrimidines?
Adenine, Guaine AG both being purines. Cytosine with thyme at are pyrimidines.
2. How is DNA informaDon used to make proteins? What are the steps of this process?
used to make proteins through transcription and translation. 1st step, when the enzymes
from the DNA molecules are transcribed into ,mRNA with help from the RNA polymease.
2nd the mRNA reaches the ribosome 3rd tRNA and translated into amino acids that
block the proteins.
3. Give an example of a scenario in which you would perform PCR vs a scenario in which you would
use recombinant DNA technology.
determining the source of an illness and could also be used to get DNA from blood samples , for
example using human growth hormones.
4. What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR
changed biotechnology?
Denaturing, annealing, and elongation. Polymerase chain reaction is a method used in
molecular biology to make millions of physical copies of a specific DNA sequence .
5. How could you take a protein with a known sequence of amino acids and use it to create an
arDficial gene?
can be done by suing recombinant DNA tech such as binoformatic tools and databases
This study source was downloaded by 100000902171055 from CourseHero.com on 12-09-2025 22:28:28 GMT -06:00
https://www.coursehero.com/file/179651155/LAB-7-pdf-pdf/
Student Name: Jennifer Baeza
Access Code (located on the underside of the lid of your lab kit): ACOF5124A
“Pre-Lab QuesCons”
1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which are
pyrimidines?
Adenine, Guaine AG both being purines. Cytosine with thyme at are pyrimidines.
2. How is DNA informaDon used to make proteins? What are the steps of this process?
used to make proteins through transcription and translation. 1st step, when the enzymes
from the DNA molecules are transcribed into ,mRNA with help from the RNA polymease.
2nd the mRNA reaches the ribosome 3rd tRNA and translated into amino acids that
block the proteins.
3. Give an example of a scenario in which you would perform PCR vs a scenario in which you would
use recombinant DNA technology.
determining the source of an illness and could also be used to get DNA from blood samples , for
example using human growth hormones.
4. What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR
changed biotechnology?
Denaturing, annealing, and elongation. Polymerase chain reaction is a method used in
molecular biology to make millions of physical copies of a specific DNA sequence .
5. How could you take a protein with a known sequence of amino acids and use it to create an
arDficial gene?
can be done by suing recombinant DNA tech such as binoformatic tools and databases
This study source was downloaded by 100000902171055 from CourseHero.com on 12-09-2025 22:28:28 GMT -06:00
https://www.coursehero.com/file/179651155/LAB-7-pdf-pdf/
