Questions and Correct Answers |
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Assay - 🧠 ANSWER ✔✔Process of determining the amount of analyte in a
sample.
Analyte - 🧠 ANSWER ✔✔The chemical substance being measured. Can be
gasses, proteins etc.
Signal - 🧠 ANSWER ✔✔Analyte + Chemical or Physical Stimulus.
, Observable change in some property.
A measured quantity that is correlated to the amount of analyte.
Qualitative analysis - 🧠 ANSWER ✔✔correlate signal to WHAT is in a
sample
Quantitative analysis - 🧠 ANSWER ✔✔correlate signal to HOW MUCH is in
a sample
Noise - 🧠 ANSWER ✔✔Unwanted variation in a measured quantity. Often
takes the form of random fluctuations in a measured signal.
Signal-to-noise ratio - 🧠 ANSWER ✔✔Magnitude of signal divided by
magnitude of noise.
S/N is proportional to the sqrt(# of measurements)
Required S/N ratio to Resolve an analytical signal - 🧠 ANSWER ✔✔S/N > 3
Detection limit - 🧠 ANSWER ✔✔The amount of analyte that corresponds to
a signal just greater than the mean of the background plus three standard
deviations of its noise:
S>= u(bkg) + 3*stdev(bkg)