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[DOMAIN 1: INFECTION CONTROL & SAFETY - 38 Questions]
1. Which of the following is the most infectious bloodborne pathogen in healthcare settings?
A) HIV
B) Hepatitis C
C) Hepatitis B
D) Syphilis
Correct Answer: C) Hepatitis B [CORRECT]
Rationale: Hepatitis B virus (HBV) is considered the most infectious bloodborne pathogen, with
a higher concentration of viral particles in blood compared to HIV. HBV can survive in dried
blood on environmental surfaces for up to 7 days, maintaining its infectivity. The risk of
occupational transmission from needlestick injury is approximately 6-30% for HBV, compared to
1.8% for HCV and 0.3% for HIV.
2. According to OSHA's Bloodborne Pathogens Standard, which of the following must an
employer provide at no cost to employees?
A) Health insurance
B) Hepatitis B vaccination
C) Life insurance
D) Disability insurance
Correct Answer: B) Hepatitis B vaccination [CORRECT]
Rationale: OSHA's Bloodborne Pathogens Standard (29 CFR 1910.1030) requires employers to
offer the Hepatitis B vaccination series to all employees who have occupational exposure to
blood or other potentially infectious materials (OPIM) at no cost to the employee. The
vaccination must be offered within 10 working days of initial assignment. Employees may
decline the vaccination but must sign a declination form.
3. What is the proper sequence for removing personal protective equipment (PPE)?
A) Mask, gown, goggles, gloves
B) Gloves, goggles, gown, mask
C) Gown, gloves, mask, goggles
, ) Goggles, mask, gown, gloves
D
Correct Answer: B) Gloves, goggles, gown, mask [CORRECT]
Rationale: The CDC-recommended sequence for PPE removal is: 1) Gloves (most
contaminated), 2) Goggles/face shield, 3) Gown, 4) Mask/respirator (last to prevent inhalation of
airborne particles during removal). This sequence prevents contamination of skin and mucous
membranes. Hand hygiene must be performed immediately after removing all PPE.
4. How long should hands be washed with soap and water when visibly soiled?
A) 10 seconds
B) 15 seconds
C) At least 20 seconds
D) 30 seconds
Correct Answer: C) At least 20 seconds [CORRECT]
Rationale: According to CDC hand hygiene guidelines, hands should be washed with soap and
water for at least 20 seconds when visibly soiled or after caring for patients with known or
suspected infectious diarrhea (C. difficile, norovirus). Alcohol-based hand rubs (ABHR) are
appropriate for routine decontamination but are not effective when hands are visibly dirty.
5. When should a sharps container be replaced?
A) When completely full
B) When ½ full
C) When ¾ full
D) Once per week regardless of fill level
Correct Answer: C) When ¾ full [CORRECT]
Rationale: Sharps containers must be replaced when they reach ¾ full to prevent overfilling,
which increases the risk of needlestick injuries. OSHA standards require sharps containers to
be puncture-resistant, leak-proof, labeled with the biohazard symbol, and kept upright.
Overfilling is a common safety violation that puts healthcare workers at risk.
6. What is the first action a phlebotomist should take after a needlestick injury?
A) Apply pressure to the wound
B) Wash the area with soap and water
C) Report to supervisor immediately
D) Apply antiseptic ointment
Correct Answer: B) Wash the area with soap and water [CORRECT]
Rationale: The immediate first aid for a needlestick or sharps injury is to wash the affected area
thoroughly with soap and water. For mucous membrane exposure (eyes, mouth, nose), flush
with water. After initial first aid, the exposure must be reported immediately to the supervisor,
and the employer's exposure control plan must be followed. Prompt washing can reduce the risk
of pathogen transmission by flushing contaminated blood from the wound.
7. Standard precautions apply to:
A) Only patients with known infections
B) Only patients in isolation
C) All patients regardless of diagnosis
D) Only patients in the emergency department
Correct Answer: C) All patients regardless of diagnosis [CORRECT]
, ationale: Standard precautions, introduced by the CDC in 1996 (replacing Universal
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Precautions), apply to all patients regardless of their suspected or confirmed infection status.
They are based on the principle that all blood, body fluids, secretions, excretions (except
sweat), non-intact skin, and mucous membranes may contain transmissible infectious agents.
This approach treats every patient as potentially infectious.
8. Which of the following is NOT part of standard precautions?
A) Hand hygiene
B) Use of PPE
C) Airborne isolation in negative pressure rooms
D) Safe injection practices
Correct Answer: C) Airborne isolation in negative pressure rooms [CORRECT]
Rationale: Airborne isolation in negative pressure rooms is a transmission-based precaution, not
a standard precaution. Transmission-based precautions (contact, droplet, airborne) are used in
addition to standard precautions for patients with known or suspected infections spread by
these routes. Standard precautions include hand hygiene, PPE use, safe injection practices,
respiratory hygiene/cough etiquette, and safe handling of contaminated equipment.
9. What does the Needlestick Safety and Prevention Act of 2000 require?
A) Mandatory reporting of all needlestick injuries to the CDC
B) Use of safety-engineered sharps devices
C) Free treatment for all needlestick injuries
D) Annual needlestick injury training only
Correct Answer: B) Use of safety-engineered sharps devices [CORRECT]
Rationale: The Needlestick Safety and Prevention Act of 2000 amended OSHA's Bloodborne
Pathogens Standard to require employers to use safer medical devices (engineered sharps
injury protections) and to solicit input from non-managerial employees in selecting these
devices. The Act also requires maintenance of a sharps injury log and annual review of
exposure control plans to incorporate new technology.
10. Where can a phlebotomist find information about chemical hazards in the workplace?
A) Patient charts
B) Safety Data Sheets (SDS)
C) Physician orders
D) Insurance forms
Correct Answer: B) Safety Data Sheets (SDS) [CORRECT]
Rationale: Safety Data Sheets (SDS), formerly known as Material Safety Data Sheets (MSDS),
provide comprehensive information about chemical hazards, including physical and health
hazards, safe handling and storage procedures, exposure controls, and emergency response
measures. OSHA's Hazard Communication Standard (HCS) requires employers to maintain
SDS for all hazardous chemicals and ensure employee access to them.
11. What is the appropriate action if a phlebotomist is exposed to blood splatter in the eyes?
A) Rub eyes to remove contamination
B) Flush eyes with water or saline for at least 15 minutes
C) Wait to see if symptoms develop
D) Apply antibiotic ointment immediately
Correct Answer: B) Flush eyes with water or saline for at least 15 minutes [CORRECT]
, ationale: For mucous membrane exposure (eyes, mouth, nose), immediate flushing with water
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or saline for at least 15 minutes is the standard first aid protocol. Rubbing the eyes can cause
further injury and increase absorption of pathogens. After flushing, the exposure must be
reported immediately, and post-exposure evaluation and follow-up must be initiated according to
the facility's exposure control plan.
12. Which bloodborne pathogen has the highest risk of chronic infection following occupational
exposure?
A) HIV
B) Hepatitis B
C) Hepatitis C
D) All have equal risk
Correct Answer: C) Hepatitis C [CORRECT]
Rationale: Following occupational exposure, Hepatitis C has the highest risk of developing
chronic infection (approximately 75-85% of exposed individuals develop chronic HCV).
However, the risk of transmission from a single needlestick is lower for HCV (1.8%) compared to
HBV (6-30%). HIV has a low transmission risk (0.3%) but nearly 100% fatality without treatment
if infection occurs.
13. What is the minimum time alcohol-based hand rub should be used for effective hand
hygiene?
A) 5 seconds
B) 10 seconds
C) 20 seconds
D) Until hands are dry
Correct Answer: D) Until hands are dry [CORRECT]
Rationale: When using alcohol-based hand rub (ABHR), the product should be applied to the
palm of one hand and rubbed over all surfaces of both hands until they are completely dry. This
typically takes about 20 seconds. The friction and contact time are essential for reducing
microbial counts. ABHR must contain at least 60% alcohol to be effective.
14. Which of the following represents a violation of sharps safety protocols?
A) Activating the safety device immediately after needle withdrawal
B) Disposing of the needle in a sharps container at the point of use
C) Recapping a needle using the two-handed technique
D) Using a safety-engineered butterfly needle
Correct Answer: C) Recapping a needle using the two-handed technique [CORRECT]
Rationale: Recapping needles using a two-handed technique is prohibited by OSHA standards
because it is a leading cause of needlestick injuries. If recapping is absolutely necessary (e.g.,
for specific procedures), a one-handed scoop technique or a mechanical device must be used.
However, the safest practice is to never recap needles and to activate safety devices
immediately after use.
15. What is the purpose of an exposure control plan?
A) To track employee vacation time
B) To eliminate employee exposure to bloodborne pathogens
C) To outline procedures for managing exposure incidents and preventing transmission
D) To document patient billing information