BIOC20 Final Exam QUESTIONS WITH ANSWERS,

BIOC20 Final Exam QUESTIONS WITH |\ |\ |\ |\ |\




ANSWERS

Negative strand RNA genomes - CORRECT ANSWERS
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✔✔Single stranded |\




Cannot be directly translated
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To make proteins, the negative strand must be used to
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make a complimentary positive strand
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How to convert negative strand to positive strand? -
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CORRECT ANSWERS ✔✔Original genome (negative sense
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strand) -> conversion using RNA dependent RNA
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polymerase -> complimentary positive sense strand |\ |\ |\ |\ |\




Positive stranded RNA virus - CORRECT ANSWERS
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✔✔Does not need an RNA polymerase, can be directly
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translated and can produce RNA and make RNA |\ |\ |\ |\ |\ |\ |\




What codes for the Viral RNA polymerase? - CORRECT
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ANSWERS ✔✔The Negative stranded RNA virus codes for
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their own RNA polymerase (they do NOT use the cell's
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polymerase bc it can only read DNA) |\ |\ |\ |\ |\ |\

,How does the RNA polymerase get created? - CORRECT
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ANSWERS ✔✔Negative strand is accompanied by the RNA
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polymerase which reads the negative strand and creates
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the positive strand. The positive strand can code for
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multiple viral proteins (including the RNA polymerase) as
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well as more complimentary negative strands
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Ribonuceleo-protein complex - CORRECT ANSWERS |\ |\ |\ |\ |\



✔✔Negative strand RNA genome + RNA polymerase (RNA
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polymerase is made up of diff components)
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Negative Stranded RNA Viral Families (list 5) - CORRECT
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ANSWERS ✔✔Paramyxoviruses |\




Rhabdoviruses
Filoviruses
Bunyaviruses
Influenza viruses (umbrella virus)
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Influenza Virus Proteins (individual segments) - CORRECT
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ANSWERS ✔✔Entire genome exists as 8 individual
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segments
Each segment codes for a different protein
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,What does the RNA polymerase complex consist of? -
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CORRECT ANSWERS ✔✔Complex consists of PA, PB1, and
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PB2


What do Influenza Virus Genome segments 1,2 and 3
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code for? - CORRECT ANSWERS ✔✔Segments 1,2,3 code
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for the components that make up the RNA polymerase
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complex


What does Segment 4 code for? - CORRECT ANSWERS
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✔✔Genome segment 4 corresponds to hemaglutinin (one |\ |\ |\ |\ |\ |\ |\



of the enveloped proteins)
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What does Segment 5 code for - CORRECT ANSWERS
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✔✔nucleocapsid proteins |\




What does segment 6 code for - CORRECT ANSWERS
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✔✔Neuraminidase (another viral protein) |\ |\ |\




What does segment 7 code for - CORRECT ANSWERS ✔✔2
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diff proteins:
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1) M2 (ion channel)
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2) M1 matrix protein
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, What does segment 8 code for? - CORRECT ANSWERS
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✔✔NS2 proteins |\




Is the Influenza virus enveloped or non enveloped? -
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CORRECT ANSWERS ✔✔Influenza is an enveloped virus |\ |\ |\ |\ |\ |\




What is a nucleocapsid in Influenza? - CORRECT
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ANSWERS ✔✔When the genome binds to the capsid |\ |\ |\ |\ |\ |\ |\ |\



proteins. However, for influenza, we refer to the capsid
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protein itself as a nucleocapsid protein (NP)
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How does Influenza enter a cell - CORRECT ANSWERS
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✔✔Dock at target cell and is taken in by vesicles that
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pinch inwards from plasma membrane. Now the virion is
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within an endosome and as the endosome matures, the
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enveloped virus fuses with the endosomal membrane to |\ |\ |\ |\ |\ |\ |\ |\



dump its nucleocapsid out into the cytoplasm
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How does Influenza fusion work? - CORRECT ANSWERS
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✔✔Viral envelope and envelope protein attached to
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envelope -> protein undergoes conformational change
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after being exposed to acidic environment -> fusion
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peptides are exposed and inserted into the endosomal
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membrane -> more viral envelope proteins cluster |\ |\ |\ |\ |\ |\ |\



together and fuse as well -> jack-knife confirmation
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occurs (proteins bend and pull in endosomal membrane -
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> creation of fusion channel for a nucleocapsid to enter
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