BIOS 312 - EXAM #2 QUESTIONS WITH
VERIFIED ANSWERS
In size exclusion chromatography, the ____________ phase is a solid and the
__________ phase is a liquid.
A. mobile; stationary
B. stationary; mobile
C. exclusion; exchange
D. exchange; exclusion - ANSWER B. stationary; mobile
Describe how a size exclusion chromatography column works. Your answer
must include appropriate use of the terms beads, molecules, buffer, and
fractions for you to receive full credit. - ANSWER In size exclusion
chromatography a column filled with porous beads will be used to create
fractions that contain a certain protein molecule. A buffer will be poured into the
column to elute the sample through the column. Smaller molecules will get
trapped in the beads while larger ones will run down and be collected.
During size exclusion chromatography of two proteins, the 30 kDa / 100 kDa
protein will be found in the last fractions collected. - ANSWER 30kDa
Describe how affinity chromatography works. Your answer must include
appropriate use of the term antibody or enzyme and column for full credit. -
ANSWER Antibodies will be present in the beads and desired protein will bind to
them trapping it. This will be added to a column, everything but the protein
desired will pass. Protein desired will bind to antibody.
During size exclusion chromatography, a molecule that is smaller than the bead
pore size will travel through ____________ .
A. the void volume
B. the pore volume
C. both the void volume and the pore volume
D. neither the void volume nor the pore volume - ANSWER C. both the void
volume and the pore volume
You have a mixture of 2 proteins. Protein A has a molecular weight of 27 kDa and
Protein B is 110 kDa. Knowing only this information, which type of
chromatography would be best for separating the two proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
, D. Affinity chromatography - ANSWER C. Size exclusion chromatography
You have a mixture of 2 proteins. Protein A is positively charged and Protein B is
negatively charged. Knowing only this information, which type of
chromatography would be best for separating the two proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
D. Affinity chromatography - ANSWER A. Ion exchange chromatography
You have a mixture of 2 proteins. Protein A is hydrophilic (polar) and Protein B is
hydrophobic (nonpolar). Knowing only this information, which type of
chromatography would be best for separating the two proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
D. Affinity chromatography - ANSWER B.Hydrophobic interaction
chromatography
You have a mixture of 2 proteins, A and B. You also have an antibody that binds
tightly to protein A but does not recognize protein B. Knowing only this
information, which type of chromatography would be best for separating the two
proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
D. Affinity chromatography - ANSWER D. Affinity chromatography
A size exclusion chromatography column is packed with beads that have an
average internal pore diameter of 60 kDa. Therefore the ____________ of this
column is 60 kDa.
A. void limit
B. column limit
C. exclusion limit
D. flow limit - ANSWER C. exclusion limit
A size exclusion chromatography column is packed with beads that have an
average internal pore diameter of 60 kDa. A 100 kDa protein will travel through
_____________.
A. the void volume
B. the pore volume
C. both the void volume and the pore volume
D. neither the void volume nor the pore volume - ANSWER A. the void volume
Explain what the "peak fraction" is in a chromatography experiment. - ANSWER
The highest concentration of the molecule.
VERIFIED ANSWERS
In size exclusion chromatography, the ____________ phase is a solid and the
__________ phase is a liquid.
A. mobile; stationary
B. stationary; mobile
C. exclusion; exchange
D. exchange; exclusion - ANSWER B. stationary; mobile
Describe how a size exclusion chromatography column works. Your answer
must include appropriate use of the terms beads, molecules, buffer, and
fractions for you to receive full credit. - ANSWER In size exclusion
chromatography a column filled with porous beads will be used to create
fractions that contain a certain protein molecule. A buffer will be poured into the
column to elute the sample through the column. Smaller molecules will get
trapped in the beads while larger ones will run down and be collected.
During size exclusion chromatography of two proteins, the 30 kDa / 100 kDa
protein will be found in the last fractions collected. - ANSWER 30kDa
Describe how affinity chromatography works. Your answer must include
appropriate use of the term antibody or enzyme and column for full credit. -
ANSWER Antibodies will be present in the beads and desired protein will bind to
them trapping it. This will be added to a column, everything but the protein
desired will pass. Protein desired will bind to antibody.
During size exclusion chromatography, a molecule that is smaller than the bead
pore size will travel through ____________ .
A. the void volume
B. the pore volume
C. both the void volume and the pore volume
D. neither the void volume nor the pore volume - ANSWER C. both the void
volume and the pore volume
You have a mixture of 2 proteins. Protein A has a molecular weight of 27 kDa and
Protein B is 110 kDa. Knowing only this information, which type of
chromatography would be best for separating the two proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
, D. Affinity chromatography - ANSWER C. Size exclusion chromatography
You have a mixture of 2 proteins. Protein A is positively charged and Protein B is
negatively charged. Knowing only this information, which type of
chromatography would be best for separating the two proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
D. Affinity chromatography - ANSWER A. Ion exchange chromatography
You have a mixture of 2 proteins. Protein A is hydrophilic (polar) and Protein B is
hydrophobic (nonpolar). Knowing only this information, which type of
chromatography would be best for separating the two proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
D. Affinity chromatography - ANSWER B.Hydrophobic interaction
chromatography
You have a mixture of 2 proteins, A and B. You also have an antibody that binds
tightly to protein A but does not recognize protein B. Knowing only this
information, which type of chromatography would be best for separating the two
proteins?
A. Ion exchange chromatography
B. Hydrophobic interaction chromatography
C. Size exclusion chromatography
D. Affinity chromatography - ANSWER D. Affinity chromatography
A size exclusion chromatography column is packed with beads that have an
average internal pore diameter of 60 kDa. Therefore the ____________ of this
column is 60 kDa.
A. void limit
B. column limit
C. exclusion limit
D. flow limit - ANSWER C. exclusion limit
A size exclusion chromatography column is packed with beads that have an
average internal pore diameter of 60 kDa. A 100 kDa protein will travel through
_____________.
A. the void volume
B. the pore volume
C. both the void volume and the pore volume
D. neither the void volume nor the pore volume - ANSWER A. the void volume
Explain what the "peak fraction" is in a chromatography experiment. - ANSWER
The highest concentration of the molecule.
