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non-competitive inhibitions effect on km and vmax? - ANSWER
-inhibitor binds
to allosteric
site
- Vmax
reduced
- Km
unaffected
- Only depends on concentration of
inhibitor
Effect of low and high temperatures in enzyme catalyzed
reaction - ANSWER -
Low: reaction rates rise with increase temp due to increase
energy of reactants
- High T: reaction rates decrease because of enzyme
denaturation
-
what are glycoproteins - ANSWER -proteins with attached
carbs. May play a role in mediating the interactions of cells with
,other cells and their nonliving environment, and in the sorting
of membrane proteins to cell compartments
glycolipids - ANSWER -lipids with attached carbs.
- Determine blood type, role in certain infectious disease,
function as receptors in normal cell function
why is membrane fluidity important to a cell - ANSWER -- allows
interactions to
take place within the
membrane
- basic cell processes (transport, cell movement, growth,
secretion, etc)
- Membrane
assembly
FRAP (fluorescence recovery after photobleaching) - ANSWER -
allows researches to follow the movements of molecules in the
membranes of living cells using the light microscope - be
familiar with this technique
· Cells labels by linkage to a fluorescent dye. Particular
membrane protein can be labeled using a specific probe, such
as a fluorescent antibody
· Small region of the surface is then irradiated to bleach the
dye molecules
· Recovery of the fluorescence in the bleached region is
followed over time · Rate of fluorescence recovery provides
a direct measure of the rate of diffusion of the mobile
molecules and the extent of fluorescence recovery provides a
,measure of the percentage of the labeled molecules that are
free to diffuse.
apical plasma membrane - ANSWER -selectively absorbs
substances from the
lumen
- Regulation of nutrient and
water intake
- Regulated
secretion
-
Protection
Lateral plasma membrane - ANSWER -- interacts with
neighbouring epithelial
cells
- Cell contact and
adhesions
- Cell
communication
basal membrane - ANSWER -- adheres to an underlying
extracellular substrate
(basement
membrane)
- Cell-substratum
contact
, - Generation of ion
gradients
two membrane proteins of RBC - ANSWER -- peripheral
proteins spectrin and
actin
spectrin - ANSWER -major component of the skeletin - flattens
RBCs as they
circulate through
capillaries
spectrin-actin network - ANSWER -gives the RBC strength,
elasticity, and
pliability
two integral proteins of RBC - ANSWER -- Band 2: passive
exchange of anions
across the
membrane
- Glycophorin A: allows RBC to repel each other and
prevent clumping
4 different processes by which substances move across
membranes -
ANSWER -- simple diffusion
through bilayer