pnb 2265 lab 7
possible treatments - ✔️✔️cold ringer's solution (4 degrees c), 0.1 mM DNP, low
sodium
what must be done to the intestinal sac for this experiment - ✔️✔️it must be inverted
why is there no gradient for the movement of glucose - ✔️✔️the sacs contain the same
glucose concentration as the external incubating solution
what must be in each beaker - ✔️✔️aerator
valid standard curve value - ✔️✔️R2= 0.98
what is x on the graph - ✔️✔️concentration of glucose
what is y on the graph - ✔️✔️absorbance
what would happen if you could only collect 100 microliters of the solution instead of
200 microliters for analysis - ✔️✔️the concentration of glucose would be twice the true
concentration
cold condition results - ✔️✔️glucose concentration would increase compared to initial,
but less than the control value (temp would slow transport but not inhibit it)
DNP condition results - ✔️✔️glucose concentration would not change (DNP inhibits
ATP, so N/K/ATPase pump wouldn't work which is necessary for glucose transport)
low sodium condition results - ✔️✔️glucose concentration would decrease, it may show
an increase but it would be less than the control (low sodium decreases sodium's
concentration gradient, slowing transport but not stopping it)
possible treatments - ✔️✔️cold ringer's solution (4 degrees c), 0.1 mM DNP, low
sodium
what must be done to the intestinal sac for this experiment - ✔️✔️it must be inverted
why is there no gradient for the movement of glucose - ✔️✔️the sacs contain the same
glucose concentration as the external incubating solution
what must be in each beaker - ✔️✔️aerator
valid standard curve value - ✔️✔️R2= 0.98
what is x on the graph - ✔️✔️concentration of glucose
what is y on the graph - ✔️✔️absorbance
what would happen if you could only collect 100 microliters of the solution instead of
200 microliters for analysis - ✔️✔️the concentration of glucose would be twice the true
concentration
cold condition results - ✔️✔️glucose concentration would increase compared to initial,
but less than the control value (temp would slow transport but not inhibit it)
DNP condition results - ✔️✔️glucose concentration would not change (DNP inhibits
ATP, so N/K/ATPase pump wouldn't work which is necessary for glucose transport)
low sodium condition results - ✔️✔️glucose concentration would decrease, it may show
an increase but it would be less than the control (low sodium decreases sodium's
concentration gradient, slowing transport but not stopping it)
