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TAMU Biology 111 Lab Exam 2 Test Study Guide 2025/2026 Accurate Questions and Correct Detailed Answers with Rationales | 100% Guaranteed Pass Newest Version

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Prepare effectively for Biology 111 Lab Exam 2 with this TAMU-focused guide. Includes accurate lab-based questions, detailed answers, and rationales for better understanding and retention.

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TAMU Biology 111 Lab Exam 2 FINAL
EXAM PREP 2026/2027 COMPLETE
QUESTIONS WITH VERIFIED
CORRECT ANSWERS | 100%
GUARANTEED PASS
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What does PCR stand for? -ANSWER✅✅✅polymerase chain reaction

What is the purpose of PCR? -ANSWER✅✅✅to amplify a specific desired fragment of
DNA. Need large copies of DNA to use for experimental use.

3 major parts of PCR -ANSWER✅✅✅Denaturation, annealing, & elongation

, Denaturation -ANSWER✅✅✅Break apart DNA

Annealing -ANSWER✅✅✅Binding of primers in PCR

Elongation -ANSWER✅✅✅Extend sequences to make essentially 2 copies of DNA

Why did we keep the pellet and not the supernatant -ANSWER✅✅✅The pellet
contains the DNA have settled to the bottom. The extra saline and debris and liquid is
the supernatant.

What was the purpose of adding the chelex beads -ANSWER✅✅✅absorbs Ions that
inhibit the function of the tact polymerase. Essentially, gets rid of stuff in the sample that
would inhibit other reactions from occurring.

Why did we use a thermocylcer? -ANSWER✅✅✅Facilitate the PCR reaction using fine
temperature control. Holds specific temperature to make sure the PCR reactions occurs
as it should.

gene theory -ANSWER✅✅✅theory that the characteristics of living things are
controlled by genes that are passed from parents to offspring

Central Dogma of DNA -ANSWER✅✅✅DNA-transcription-RNA-translation-protein

DNA -ANSWER✅✅✅deoxyribonucleic acid

what method did we use to measure PCR -ANSWER✅✅✅Gel electrophoresis

why are the bands in the gel in the specific place they are? -ANSWER✅✅✅DNA in
wells on negative end. Electrical current pulls DNA towards positive pole. The smaller
fragments move further and faster.

What was the purpose of a ladder? -ANSWER✅✅✅Baseline to compare other
individual samples too. If the ladder doesnt show up, it could be an issue with
experiment set up. If DNA does not show up but the ladder does, it could be an issue
with DNA.

How many nucleotides make up 1 amino acid? -ANSWER✅✅✅3

allosteric regulation -ANSWER✅✅✅The binding of a regulatory molecule to a protein
at one site that affects the function of the protein at a different site than the active site.

what was the enzyme we looked at in the Gene expression lab? -ANSWER✅✅✅Beta
gal

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