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1. The most widely accepted QC test to measure proba- CD34+ cell enu-
ble Hematopoietic Progenitor Cell (HPC) engraftment meration is the
is: best way to eval-
uate HPC engraft-
ment
2. In an emergency situation, what type of blood should Group O, Rh-neg-
be given to a female patient of child-bearing age if the ative red cells
ABO group and Rh type are unknown? should be given
3. Cryoprecipitated AHF must contain a minimum of 80 IU
how many international units of Factor VIII
4. NAT testing is required for this infectious agent HBV
5. To screen for HBV what must happen Nucleic acid test
required for each
donation
6. A patient's record shows a previous anti-Jkb, but give Jk(b-), cross-
the current antibody screen is negative. What further match compatible
testing should be done before transfusion blood
7. ABO-hemolytic disease of the newborn is usually seen
only in the new-
born of group O
mothers
8. Irradiation of a unit of Red Blood Cells is done to lymphocytes
prevent the replication of donor
9. Criteria determining Rh immune globulin eligibility mother has not
include been previously
immunized to the
D antigen
10. Which of the following practices has been useful in use of Fresh
reducing the incidence of Transfusion Related Acute Frozen Plasma
Lung Injury (TRALI) from male donors
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11. A mother is Rh-negative and the father Rh-positive. the father is het-
Their baby is Rh-negative. It may be concluded that erozygous for D
12. A blood donor has the genotype: hh, AB. Using anti-A O
and anti-B antisera, the donor's red cells will type as
group
13. Which characteristics are true of all 3 of the following detected at IAT
antibodies: anti-Fya, anti-Jka, and anti-K? phase and may
cause hemolytic
disease of the fe-
tus and newborn
(HDFN) and trans-
fusion reactions
14. During initial investigation of a suspected hemolyt- no further serolog-
ic transfusion reaction, it was observed that Blood ical testing is nec-
Bank paperwork and patient sample and blood com- essary
ponent labels were correct, the posttransfusion re-
action plasma was yellow as was the pretransfusion
sample, and the direct antiglobulin test was negative.
Repeat ABO typing on the posttransfusion sample
confirmed the pretransfusion results. What is the next
step in this investigation?
15. Red Blood Cells, Leukocytes Reduced must be 1-6°C
stored at
16. Which of the following is a characteristic of anti-i? reacts best at
room temperature
or 4°C
17. When untreated indicator cells are used in the rosette mother has a larg-
test, a positive result occurs when there are >6 er than normal
rosettes/5 fields. In this case, the results indicate that FMH
the mother had a larger than normal (e10 mL) fetal ma-
ternal hemorrhage (FMH). Additional testing needs to
be performed to calculate the FMH size.
18.
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The Donath-Landsteiner test is diagnostic for PCH. paroxysmal cold
The antibody is IgG and is biphasic: hemolysis oc- hemoglobinuria
curs when the antibody is incubated with cells and (PCH)
cold temperatures and then incubated at 37°C. Often
the antibody demonstrates specificity towards the
high-incidence antigen P (not to be confused with
P1). The antibody screen is usually negative and the
patient's red cells are coated with complement.
19. Which of the following phenotypes will react with rr
anti-f?
20. An antibody that causes in vitro hemolysis and reacts anti-Lea
with the red cells of 3 out of 10 AHG-crossmatched
donor units is most likely
Lewis antibodies may bind complement and fresh
serum that contains anti-Lea may hemolyze Le(a+)
red cells in vitro. Approximately 22% of the popula-
tion is Le(a+)
21. Ax cells are more strongly reactive with anti-A,B than anti-A zero, anti-B
with anti-A and the plasma frequently has anti-A1 zero, anti-AB 2+
present.
22. Upon inspection, a unit of Apheresis Platelets is not- quarantine for
ed to have visible clots, but otherwise appears nor- Gram stain and
mal. The technologist should: culture
23. The most appropriate laboratory test for early detec- visual inspection
tion of acute posttransfusion hemolysis is for free plasma he-
moglobin
24. What is the most important consideration for a pa- determine the
tient requiring red cell transfusion due to severe ane- presence of un-
mia when their serum contains a warm autoantibody derlying alloanti-
body(ies)
25.
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