GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf
GMS 6552 Exam 2
ACE inhibitors - ANS-"PRIL"
Captopril
Enalapril
Lisinopril
Fosinopril
acetycholine receptors - ANS-muscarinic and nicotinic
Aerobic glycolysis inhibitors - ANS-deregulating cellular energetics
Aldosterone Antagonists - ANS-Spironolactone
Eplerenone
alpha 1 selective antagonists - ANS-Prazosin
Doxazosin
Amplification - ANS-Multiple gene copies = too much transcript and protein
DNA sequencing, DNA-PCR
RNA sequencing, RNA-PCR
Protein: Western
ARBs - ANS-"Sartan"
Losartan
Valsartan
Candesartan
Olmesartan
Arterial vasodilators - ANS-Calcium blockers (Dihydropyridines, Diltiazem, Verapamil)
Hydralazine
Diazoxide
Minoxidil
Basis of separation by electrophoresis - ANS-Charged ions migrate in electric field (protein -)
Alter pore size of gel to suit sample
Benzodiazepines - ANS-GABAa receptor activation
simple partial, grand mal anti-seizure profile
Beta 1 selective blockers - ANS-metoprolol
atenolol
GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf
, GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf
nebivolol
Biochemical functions of oncogenes - ANS-G-proteins (Ras)
Protein Kinases (Raf, Akt)
Lipid Kinases (PI3-Kinase)
Transcription Factors (Myc)
Binding and inactivating apoptotic proteins (BCL2)
Protein Kinase regulatory subunits (Cyclins A, E, D)
Transcriptional coactivators (B-Catenin, YAP, TAZ)
Blotting - ANS-Primary antibody binds target protein
Secondary antibody binds primary antibody and is conjugated to an enzyme for detection (HRP)
Add detection reagent
Enzyme converts reagent to detectable signal
Visible or fluorescent
Both venous and arterial vasodilators - ANS-Nitroprusside
ACE inhibitors
ARBs
Alpha blockers
Combination of hydralazine and isosorbide dinitrate
Cancer cells addicted to oncogenes - ANS-If Ras is inactivated in Myc transformed cancers they
die
If a single oncogene is driving a given cancer then inhibiting the function of that oncogene will
cure the cancer
Treatment of breast cancer patients whose tumors harbor the HER2 oncogene is an effective
way of treating these cancers
Carbamazepine - ANS-sodium channel blocker
simple partial, grand mal anti-seizure profile
Cell membrane - ANS-functions as a capacitor, the cell's electrical potential is the charge on the
capacitor
Cell Viability - ANS-MTT assay
3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide
Converted to colored, insoluble form in viable cells
cell-attached recording - ANS-mild suction & tight contact between pipette and membrane
allows experimental control of the membrane potential to characterize the voltage dependance
of membrane currents
GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf
GMS 6552 Exam 2
ACE inhibitors - ANS-"PRIL"
Captopril
Enalapril
Lisinopril
Fosinopril
acetycholine receptors - ANS-muscarinic and nicotinic
Aerobic glycolysis inhibitors - ANS-deregulating cellular energetics
Aldosterone Antagonists - ANS-Spironolactone
Eplerenone
alpha 1 selective antagonists - ANS-Prazosin
Doxazosin
Amplification - ANS-Multiple gene copies = too much transcript and protein
DNA sequencing, DNA-PCR
RNA sequencing, RNA-PCR
Protein: Western
ARBs - ANS-"Sartan"
Losartan
Valsartan
Candesartan
Olmesartan
Arterial vasodilators - ANS-Calcium blockers (Dihydropyridines, Diltiazem, Verapamil)
Hydralazine
Diazoxide
Minoxidil
Basis of separation by electrophoresis - ANS-Charged ions migrate in electric field (protein -)
Alter pore size of gel to suit sample
Benzodiazepines - ANS-GABAa receptor activation
simple partial, grand mal anti-seizure profile
Beta 1 selective blockers - ANS-metoprolol
atenolol
GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf
, GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf
nebivolol
Biochemical functions of oncogenes - ANS-G-proteins (Ras)
Protein Kinases (Raf, Akt)
Lipid Kinases (PI3-Kinase)
Transcription Factors (Myc)
Binding and inactivating apoptotic proteins (BCL2)
Protein Kinase regulatory subunits (Cyclins A, E, D)
Transcriptional coactivators (B-Catenin, YAP, TAZ)
Blotting - ANS-Primary antibody binds target protein
Secondary antibody binds primary antibody and is conjugated to an enzyme for detection (HRP)
Add detection reagent
Enzyme converts reagent to detectable signal
Visible or fluorescent
Both venous and arterial vasodilators - ANS-Nitroprusside
ACE inhibitors
ARBs
Alpha blockers
Combination of hydralazine and isosorbide dinitrate
Cancer cells addicted to oncogenes - ANS-If Ras is inactivated in Myc transformed cancers they
die
If a single oncogene is driving a given cancer then inhibiting the function of that oncogene will
cure the cancer
Treatment of breast cancer patients whose tumors harbor the HER2 oncogene is an effective
way of treating these cancers
Carbamazepine - ANS-sodium channel blocker
simple partial, grand mal anti-seizure profile
Cell membrane - ANS-functions as a capacitor, the cell's electrical potential is the charge on the
capacitor
Cell Viability - ANS-MTT assay
3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide
Converted to colored, insoluble form in viable cells
cell-attached recording - ANS-mild suction & tight contact between pipette and membrane
allows experimental control of the membrane potential to characterize the voltage dependance
of membrane currents
GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf GMS 6552 Exam 2.pdf
