PNB 2265 LAB 8 TRANSPORT EXAM
QUESTIONS WITH VERIFIED SOLUTIONS .
microvilli structure - Correct Answer -for the brush border and contain enzymes
that complete digestion
what do brush border enzymes do in regards to glucose - Correct Answer -break
down disaccharides (sucrose, lactose, maltose) into monosaccharides (glucose,
fructose, galactose)
what is inside villi - Correct Answer -lacteal and capillary network
what are the types of membrane transport - Correct Answer -active transport:
using ATP to transport molecules against their concentration gradient
-primary: uses energy from ATP directly
-secondary: uses energy from concentration gradient created by primary active
transport
passive: transporting a molecule down its concentration gradient using no ATP
(non-polar and lipophilic molecules)
facilitated diffusion: passive transport with a carrier protein (glucose/amino
acids)
what are the 3 mechanisms for glucose transport? - Correct Answer -1- primary
active transport via Na/K pump in the basal membrane
2- secondary active transport by Na/ glucose transporter in apical membrane
1
, 3- facilitated diffusion by glucose transporter in the basal membrane
(kinda in this order)
what does the Na/K pump do? - Correct Answer -creates a concentration
gradient by moving 3 Na out and 2 K+ in
maintains low intracellular Na (so that it favors the movement of Na in, and the
Na/glucose transporter can work)
what is another name for the Na/glucose transporter? how does it work - Correct
Answer -SGLT
-it is a symporter and brings in Na down its concentration gradient, with glucose
what animal and body part do we use in lab? how is it prepared? - Correct
Answer -mouse small intestine
it is everted so that the mucosa is on the outside and serosa is on the inside
usually glucose goes from the lumen to the outside, but in this case glucose will
be transported into the sac
what are the different variables tested in the lab - Correct Answer -putting the
intestinal sac in a beaker with different components
control- 37 C
cold- 4 C
DNP (ATP production inhibitor), 37C
low Na, 37C
2
QUESTIONS WITH VERIFIED SOLUTIONS .
microvilli structure - Correct Answer -for the brush border and contain enzymes
that complete digestion
what do brush border enzymes do in regards to glucose - Correct Answer -break
down disaccharides (sucrose, lactose, maltose) into monosaccharides (glucose,
fructose, galactose)
what is inside villi - Correct Answer -lacteal and capillary network
what are the types of membrane transport - Correct Answer -active transport:
using ATP to transport molecules against their concentration gradient
-primary: uses energy from ATP directly
-secondary: uses energy from concentration gradient created by primary active
transport
passive: transporting a molecule down its concentration gradient using no ATP
(non-polar and lipophilic molecules)
facilitated diffusion: passive transport with a carrier protein (glucose/amino
acids)
what are the 3 mechanisms for glucose transport? - Correct Answer -1- primary
active transport via Na/K pump in the basal membrane
2- secondary active transport by Na/ glucose transporter in apical membrane
1
, 3- facilitated diffusion by glucose transporter in the basal membrane
(kinda in this order)
what does the Na/K pump do? - Correct Answer -creates a concentration
gradient by moving 3 Na out and 2 K+ in
maintains low intracellular Na (so that it favors the movement of Na in, and the
Na/glucose transporter can work)
what is another name for the Na/glucose transporter? how does it work - Correct
Answer -SGLT
-it is a symporter and brings in Na down its concentration gradient, with glucose
what animal and body part do we use in lab? how is it prepared? - Correct
Answer -mouse small intestine
it is everted so that the mucosa is on the outside and serosa is on the inside
usually glucose goes from the lumen to the outside, but in this case glucose will
be transported into the sac
what are the different variables tested in the lab - Correct Answer -putting the
intestinal sac in a beaker with different components
control- 37 C
cold- 4 C
DNP (ATP production inhibitor), 37C
low Na, 37C
2
