Exam and All Actual Answers 2025-
2026 Updated.
this name is unique to each species and consists of a genus followed by a species name -
Answer binomial name
an organism, which has not yet been officially named - Answer strains
show how one organism is related to another one - Answer phylogenetic tree
contains nutrients, such as carbon and nitrogen, required for cells to grow - Answer culture
medium
composed of digest of chemically undefined substances such as yeast and meat extracts -
Answer complex medium
on for which a precise chemical composition is known - Answer defined medium
used for isolation and growth of microorganisms, solid media in a petri dish - Answer plates
test tubes containing solid medium, which has been allowed to solidify in an upright position,
and are usually used for anaerobic growth - Answer deeps
deeps are usually used for what? - Answer anaerobic growth
test tubes continuing sold medium that has been allowed to solidify at an angle and the surface
of the agar is used for the maintenance of stock cultures of microorganisms - Answer slants
slants are usually used for what? - Answer the maintenance of stock cultures of
microorganisms
the killing or removal of all living organisms and their viruses from a growth medium - Answer
sterilization
a sealed device that allows the entrance of steam under pressure - Answer autoclave
, the autoclave is heated at what temperature? - Answer 121 deg C at 15 lb per square inch
kills bacteria by oxidative effects - Answer Dry heat sterilization
What are autoclaves generally used for? - Answer sterilize liquid media and other items that
are heat resistant
one of the simplest methods of dry heat sterilization, used to sterilize inoculating loops - Answer
flaming
items to be sterilized are placed in an oven and heated to 170 deg C for 2 hours - Answer hot
air sterilization
Hot air sterilization is used to sterilize what? - Answer glassware
a device with pores too small for the passage of microorganisms but large enough to allow
passage of the liquid or gas - Answer filtration
What is an example of an item sterilized by filtration? - Answer beer
Heat sensitive objects like plastic petri dishes are sterilized using what method? - Answer
gaseous chemosterilizers
What is a widely used gas for chemosterilization? - Answer ethylene oxide
a method of sterilization used to sterilize wood, metal, and rubber products where no heat is
used in the process. - Answer cold sterilization
includes microwaves, UV radiation, X-rays, gamma rays and electrons; type of sterilization -
Answer electromagnetic radiation
causes damage to DNA (thymine dimers) leading to death of exposed organisms - Answer UV
radiation
causes ions and other reactive molecules to be produced and these reactive molecules can
degrade or alter biopolymers such as DNA and proteins - Answer ionizing radiation