LSU BIOL 2051 LAB Experiment 9
Questions and Answers.
Metabolism: - Answer sun of reactions that are associated with the life of a cell
Enzymes: - Answer proteins that serve as biological catalysts
What would happen if we didn't have enzymes? - Answer None of the reactions associated
with living cells would occur at a rapid enough rate for life as we know it to exist.
Substrate: - Answer substance that an enzyme reacts with to catalyze a certain reaction
True or false?: An enzyme can catalyze multiple types of reactions. - Answer False; can only
catalyze one
True or false?: An enzyme can only react with one substrate. - Answer True
Extracellular Enzymes: - Answer enzymes that act outside of the cell
Function of extracellular enzymes: - Answer degrade molecules into units that are small
enough to be transported by proteins across the cell membrane and into the cell
True or false?: Extracellular enzymes are useful inside and outside of the cell. - Answer False;
only on the inside
Endoenzymes: - Answer enzymes that act within the cell
Function of endoenzymes: - Answer Synthesize molecules that are needed by the cell.
Degrade molecules to simpler molecules and release energy.
True or false?: All enzymes are always produced. - Answer False; some enzymes are only
produced when the appropriate substrate is available
Inducible/Adaptive Enzymes: - Answer enzymes that are produced only if the appropriate
substrate is available
, Constitutive Enzymes: - Answer enzymes that are produced at all times
Why are constitutive enzymes produced at all times? - Answer because they are essential to
the cell
Morphology: - Answer to examine the cell size, shape, and arrangement of a bacterium
Physiology: - Answer how an organism functions in an environment
What can tell microbiologist do to study the physiology of an organism? - Answer investigate
the organism's metabolic capabilities
What can investigating an organism's metabolic capabilities tell microbiologists? - Answer
How it reacts biochemically.
Things about the enzymes it produces.
What enzyme production lets microbiologists know that an organism produces energy using
aerobic respiration? - Answer production of oxidase and catalase enzymes
What provides the energy to build the H+ gradient that leads to ATP production? - Answer
Redox Reactions
Last electron acceptor in aerobic respiration: - Answer Oxygen
Why does the oxygen accept the electron at the end of the ETC? - Answer To be reduced
Oxidase Assay: - Answer tests for the presence of cytochrome c oxidase
What is the oxidase assay most often used to differentiate between? - Answer The genera
Neisseria and Moraxella from Acinetobacter.
Pseudomonas and Vibrio from Escherichia and Enterobacter.
Between the genera Neisseria & Moraxella and Acinetobacter, which are oxidase positive and
which are oxidase negative? - Answer Neisseria & Moraxella are positive.
Acinetobacter is negative.
Questions and Answers.
Metabolism: - Answer sun of reactions that are associated with the life of a cell
Enzymes: - Answer proteins that serve as biological catalysts
What would happen if we didn't have enzymes? - Answer None of the reactions associated
with living cells would occur at a rapid enough rate for life as we know it to exist.
Substrate: - Answer substance that an enzyme reacts with to catalyze a certain reaction
True or false?: An enzyme can catalyze multiple types of reactions. - Answer False; can only
catalyze one
True or false?: An enzyme can only react with one substrate. - Answer True
Extracellular Enzymes: - Answer enzymes that act outside of the cell
Function of extracellular enzymes: - Answer degrade molecules into units that are small
enough to be transported by proteins across the cell membrane and into the cell
True or false?: Extracellular enzymes are useful inside and outside of the cell. - Answer False;
only on the inside
Endoenzymes: - Answer enzymes that act within the cell
Function of endoenzymes: - Answer Synthesize molecules that are needed by the cell.
Degrade molecules to simpler molecules and release energy.
True or false?: All enzymes are always produced. - Answer False; some enzymes are only
produced when the appropriate substrate is available
Inducible/Adaptive Enzymes: - Answer enzymes that are produced only if the appropriate
substrate is available
, Constitutive Enzymes: - Answer enzymes that are produced at all times
Why are constitutive enzymes produced at all times? - Answer because they are essential to
the cell
Morphology: - Answer to examine the cell size, shape, and arrangement of a bacterium
Physiology: - Answer how an organism functions in an environment
What can tell microbiologist do to study the physiology of an organism? - Answer investigate
the organism's metabolic capabilities
What can investigating an organism's metabolic capabilities tell microbiologists? - Answer
How it reacts biochemically.
Things about the enzymes it produces.
What enzyme production lets microbiologists know that an organism produces energy using
aerobic respiration? - Answer production of oxidase and catalase enzymes
What provides the energy to build the H+ gradient that leads to ATP production? - Answer
Redox Reactions
Last electron acceptor in aerobic respiration: - Answer Oxygen
Why does the oxygen accept the electron at the end of the ETC? - Answer To be reduced
Oxidase Assay: - Answer tests for the presence of cytochrome c oxidase
What is the oxidase assay most often used to differentiate between? - Answer The genera
Neisseria and Moraxella from Acinetobacter.
Pseudomonas and Vibrio from Escherichia and Enterobacter.
Between the genera Neisseria & Moraxella and Acinetobacter, which are oxidase positive and
which are oxidase negative? - Answer Neisseria & Moraxella are positive.
Acinetobacter is negative.
