Chapter
2
Gene Cloning
,Learning Objectives:
1. Explain the steps for gene cloning
2. Compare selection and screening
processes involved in gene cloning
3. Explain the reasons for doing gene
cloning
, Recombinant DNA Technology and
Gene cloning
Gene cloning:
• Separation of specific gene or DNA fragments from
a donor cell, attaching it to a vector (carrier
molecule) and replicating the recombinant
vector in a host cell.
• Became a reality in 1970
2
Gene Cloning
,Learning Objectives:
1. Explain the steps for gene cloning
2. Compare selection and screening
processes involved in gene cloning
3. Explain the reasons for doing gene
cloning
, Recombinant DNA Technology and
Gene cloning
Gene cloning:
• Separation of specific gene or DNA fragments from
a donor cell, attaching it to a vector (carrier
molecule) and replicating the recombinant
vector in a host cell.
• Became a reality in 1970
