3A : CELL STRUCTURE
Observing cells
Cells
·
are biological functional Units
of organisms
used because
Microscopes are to observe cells of their small size
-
There two
types of
:
are
microscopes
-
>
Light Coptical)
> Electron
I
Light Electron
Electron beams used to
Light is used to illuminate are
· ·
the illuminate the
sample sample
·
Low resolution (wavelength of light) ⑧
High resolution (wavelength of electrons)
Used live May kill the live
for samples sample
· ·
Cheaper Expensive
· ·
false
Portable light images Not
+
portable
· ·
If due to
staining
May produce artefacts
[
·
staining
·
staining is used to see the inside of thecell
Different stains used
for different types of sample
-
are
>
haematoxylin-stains the nuclei
blue brown
-purple , or
>
methylene blue-stains animal nuclei
-
blue
> acetocarmine -
stains chromosomes
>
iocline starch
stains
containing material
-
-
blue black
Magnification
magnification= Image size
total objective (ens lens
magnification X
eye piece
:
Actual size
Image Size :
Actual Size -
magnification um-mierometre
Actual size :
Image Size -
X10-5
magnification
, Eukaryotic Cells
Eukaryotic cells are
complex
-
They have a membrane-bound nucleus
Structures
① Membrane ⑧ Rough endoplasmic reticulum (RER)
Localises -involved production and
enzymes reaction
pathways in
-
in
transport of proteins
②
Protoplasm
The ⑨ Smooth
cytoplasm and nucleus combined
endoplasmic reticulum
-
-involved the and
in
synthesis
③ Nucleus
transport of steroids and
contains DNA RNA and
protein lipids
-
,
-
surrounded by a double nuclear membrane
with
pores 10 Golgi apparatus
-
stacks extracellular made
& Mitochondria membranes and
packages
site for them transport
anaerobic
respiration for
-
releases ATP molecules
-
⑪ Centrosomes
③ Centrioles -
organises the microtubles and
-
involved in cell division provides structure to the cell
⑥ Ribosomes
20s
-consists ribosomal RNA
of
-
made
of 60s and 40s
up
Site
for protein Synthesis
-
70s
-
-
found in Mitochondria
① Lysosomes
contain digestive enzymes apoptosis
-
&
-
breaks down worn-out cells or
digests food
*
simple organisms
, Extracellular Enzymes
·
organelles are
specialised parts of a cell that
carry out a
paticular function
membrane bound
some
organelles are
-
involved and include
organelles in the
production secretion
of proteins :
> Nucleus
Transcription of DNA
-
> Ribosomes
Translation to
produce proteins
-
Endoplasmic transports proteins
+
>
Rough Reticulum Synthesises
-
>
Golgi Apparatus prepares (packages) protein for transport
-
Cell Membrane
>
Surface
-
vesicle
containing protein fuses with the
surface membrane for secretion
(RER)
Rough Endoplasmic Reticulum
secreted become
proteins produced can
The be out the cell
of or
attached to the cell
surface membrane inside
space of
↓ the RER
Proteins are
folded and
processed in the lumen
Ribosomes that the
in the
cytoplasm synthesise proteins stay within
-
Cell
Golgi Apparatus
Processed RER
transported to the
proteins from the are
Golgi apparatus
-
in Vesicles
They fuse with the Golgi apparatus
-
This releases the the Golgi
proteins into
-
The Golgi apparatus modifies the them
proteins preparing for secretion
-
,
Proteins that in the
Golgi apparatus are
usually
-
go
:
>
Exported
Put into
lysosomes
Delivered to membrane-bound
>
organelles
The
large called
·
movement of a molecule out
of
a cell
using a vesicle is
exocytosis