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Bio 261 Exam 2 (Powell) Study Guide with Actual Answers Updated.

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Activator protein - Answer a protein that activates the expression of the gene by stimulating the RNA polymerase to bind to the promoter region. Hence, it brings about positive control of the gene; stimulates transcription recognize - Answer Activator protein helps RNA polymerase ______ promoter. -may bend DNA structure -may interact directly with RNA polymerase Allosteric protein - Answer - a protein containing an active site for binding substrate and an allosteric site for binding an effector molecule such as the end product of a biochemical pathway attentuation - Answer a mechanism for controlling gene expression that terminates transcription after initiation but before a full-length messenger RNA is produced Autoinducer - Answer - a small signal molecule that takes part in quorum sensing -diffuses freely across the cell envelope -reaches high concentrations inside cell only if many cells are nearby and making the same -binds to specific activator protein or sensor kinase, triggering transcription of specific genes Aliivibrio fischeri - Answer Quorum sensing first discovered as mechanism regulating light production in bacteria including ______ Global control systems - Answer ________: regulate expression of many different genes simultaneously (e.g., lactose operon and maltose regulon) Catabolite repression - Answer the suppression of alternative catabolic pathways by a preferred source of carbon and energy -controls use of carbon sources if more than one is present -synthesis of unrelated catabolic enzymes (e.g., lactose operon & maltose regulon) is repressed if glucose is present in growth medium -is an example of global control

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BIO 261
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BIO 261

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Microbiology: Exam 1 Study Guide
Questions with All Correct Answers
2025-2026 Updated.
microorganisms - Answer life forms too small to be seen by the human eye



bacteriology - Answer the study of bacteria



mycology - Answer the study of fungi



pathogens - Answer organisms that cause disease



culture media - Answer cells grown in/on nutrient medium



growth - Answer increase in cell number resulting from cell division



infection - Answer microorganism is growing in the body



cytoplasmic membrane; cytoplasm; ribosomes - Answer What do all cells have in common?



peptidoglycan, pseudomurein, chitin, cellulose - Answer What are some potential molecules
that make up cell walls?



Mycoplasma - Answer What genus bacteria has no cell wall?



genome - Answer a cell's full set of genes



eukaryotic has linear DNA; prokaryotic has a single circular chromosome and may also have
plasmids - Answer What is the difference between eukaryotic and prokaryotic DNA?



morphology - Answer cell size and shape



Cyanobacteria - Answer What bacteria was responsible for the oxygenation of Earth?

, agents of disease, bioremediation, vaccination and antibiotic therapy, water treatment, food
safety, microflora, fermentation, biotechnology - Answer What are some of the functions of
microorganisms in human life?



industrial microbiology - Answer use of microorganisms for major industries such as
pharmaceuticals and brewing



biotechnology - Answer genetically engineered microbes making high-value products in
small amounts



bioremediation - Answer using microorganisms to clean up pollutants



Robert Hooke - Answer first to observe "small chambers" in cork and call them cells.



Antoni van Leeuwenhoek - Answer discovered bacteria with the microscope, calling them
small animalcules. Created his own microscopes in order to look at bacteria.



basic dyes - Answer positively-charged dyes that bind strongly to charged cell components,
such as methylene blue, crystal violet, and safranin



differential stains - Answer use a primary stain and a counterstain to distinguish cell types or
parts



phase-contrast microscopy - Answer Allows examination of living organisms and internal cell
structures

Brings together two sets of light rays, direct rays, and diffracted rays to form an image. Result is
dark cells on a light background.



dark-field microscopy - Answer a form of microscopy in which the specimen is brightly
illuminated on a dark background. Excellent for observing motility in microorganisms.



fluorescence microscopy - Answer uses a fluorescent dye that emits fluorescence when
illuminated with ultraviolet radiation. Cells appear to glow. Helpful in clinical diagnostic
microbiology for enumerating bacteria.



transmission electron microscopy - Answer An electron microscope used to study the
internal structure of thin sections of cells

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