ASCP Molecular Biology Certification Exam Questions and Answers 100% Solved, Updated this Year

ASCP Molecular Biology Certification
Exam Questions and Answers 100%
Solved, Updated this Year
PyrimidineA-AAnswer✓AOneAcarbonAring

Cytosine,AThymine,AUracil


WhatAisAtheAfunctionAofAmRNA?A-AAnswer✓ACarriesAgeneticAinfoAoutAofAnucleus

TranscriptAtranslatedAtoAprotein


WhatAisAtheAfunctionAofAtRNA?A-AAnswer✓ACarriesAaaAtoAribosome
AnticodonApairsAwithAcodonAonAmRNAAstrand


WhatAisAtheAfunctionAofArRNA?A-AAnswer✓ApartAofAribosomeAstructure
mostAabundantARNA
coordinatedAcouplingAofAtRNAAtoAmRNAAcodons


FeedbackAinhibitionA-AAnswer✓AProductAofApathwayAisAnoncompetitiveAinhibitor

BindsAtoAallostericAsiteAtoAslowAdownArxnAb/cAtooAmuchAproduct


EndonucleasesA(Prok)A-AAnswer✓ARestrictionAenzymes

CleavesAphoshpodiesterAbondsAw/iApoly-ntAchain
RecognitionAsiteAisApalindromicAsequence
TypesAI-V


ORIAsitesA-AAnswer✓AntAsequenceAwhereAreplicationAisAinitiated



TopoisomeraseAIA-AAnswer✓AInducesAssAbreaks
RemoveADNAAsupercoilsAduringATXNAandADNAAreplication;AforAstrandAbreakageAduringArecombinatio
n;AforAchrAcondensation;AandAtoAdisentangleAintertwinedADNAAduringAmitosis

,topoisomeraseAIIA-
AAnswer✓AcutsAbothAstrandsAofAoneADNAAdoubleAhelix,ApassesAanotherAunbrokenADNAAhelixAthr
oughAit,AandAthenAreannealsAtheAcutAstrands


GyraseA(topoisomeraseAII)A-
AAnswer✓AUnwindsAsupercoilingAcausedAbyAunwindingAatAtheArepAforkAbyAintroducingADSBs



HelicaseA-AAnswer✓ABreaksAH-bondsAofAdoubleAhelixAatAtheAreplicationAfork



single-strandADNAAbindingAproteinsA(SSBPs)A-AAnswer✓ABindsAssDNAAandApreventsAitAfromAre-
annealingAduringATXN,Areplication,Arepair,AandArecombination


TelomeresA-
AAnswer✓ARepeatAsequenceA(TTAGGG)AatAtheAendsAofAchr,AprotectAchrAfromAdegradation



RNAApolymeraseA-AAnswer✓ADNAAdependentARNApol
TranscribesADNAAtemplateAtoARNAA(3'-->5';Aanti-parallel)


SplicesomesA-AAnswer✓AComplexAofAsnRNPs

RemovesAintronsAfromApre-mRNAAandAsplicesAexonsAtogether


EnhancersA-
AAnswer✓AShortAregionsAofADNAAthatAbindAproteinsA(TXNAfactors)AthatAenhanceATXNAofAaAgene



ReverseAtranscriptaseA-AAnswer✓AenzymeAthatAtranscribesARNAAtoAcDNAA(lacksAintrons)
RNAA-->ARNA:DNAA-->AcDNAA(dsDNA)


OpenAReadingAFrameA(ORF)A-
AAnswer✓AsectionsAofADNAAthatAbeginAwithAstartAcodonsAandAendAwithAstopAcodons
DNA:A5'A-->A3'
transcription:A3'A-->A5'ADNAA-->ARNAA(promoter)
translation:A5'A-->A3'AmRNA


SpectrophotometerA-AAnswer✓AMeasuresAamountAofAlightAabsorbed

,QuantitativeAmeasurementAofA[DNA/RNA]


AtAwhatAwavelengthAdoesADNAAandARNAAabsorb?A-AAnswer✓A260Anm



AtAwhatAwavelengthAdoesAproteinAabsorb?A-AAnswer✓A280Anm



OrganicAisolationAmethodA-AAnswer✓A1.ALyse

2.AAddAphenol/AchloroformA>Avortex/spin
3.ATransferAaqueousAlayerA(top)AtoAnewAtube
4.AAddAchloroform:IAAA(removesAphenol)A>Avortex/spin
5.ATransferAaqueousAlayerAtoAnewAtube
6.AAddANaOAcAandAEtOHA>Avortex/spin
7.ADecant
8.AResuspend


WhatAdoesAtheAincubationAstepAinAhybridizationAdo?A-
AAnswer✓AAllowsAformationAofAdsAmolecules



FormamideAactsAasAaA__________AinAaAhybridization.A-AAnswer✓AdenaturingAagent



LineAProbeAAssayA(LiPA)A-AAnswer✓AreverseAhybridizationAassayAusingAsequence-
specificAoligonucleotideAprobesA(reverseASSOP)
multi-parameterAtestingA-->AsingleAstrip


Single-StrandedAConformationalAPolymorphismAAnanlysisA(SSCP)A-
AAnswer✓AUsedAforAknownAgene,AunknownAmut

MutationAscreening
ShortAPCRAproductsAformA3DAconformationAwhenAcooledA--
>AmutsAhaveAdifferentAconformationAthanAWT
Non-denaturingAPAGE,AmutsAmigrateAdifferentAthanAWT


HowAdoesAEtBrAcauseADNAAtoAfluoresce?A-AAnswer✓AIntercalatesAintoAtheAdoubleAhelix
AbsorbsAUVA~300Anm,AemitsA~600Anm

, PulseAFieldAGelAElectrophoresisAstepsA-AAnswer✓A1.Aculture

2.AembedApelletAinAagaroseAplug
3.AtreatAw/AlysozymeA(cellAlysis)
4.AproteinaseAK
5.Agel


WhatAareAtheA3AstepsAofAPCRAandAtheirAtemperatures?A-AAnswer✓ADenatureA90-96C

AnnealA50-70C
ExtensionA68-75C


BisulfiteADNAAsequencing/MethylationAspecificA-AAnswer✓A1.AREAdigest
2.AElectrophoreseAandApurifyAfragmentAofAinterest
3.ADenatureAandAincubateAw/AsodiumAbisulfateA(turnsAC>U,AmethylatedACAisAunchanged)
4.Aclean,Appt,AandAresuspend
5.APCRA-->Asequence
6.ACompareAtreatedAvsAuntreated,AnoteAwhereACGAareAnotAchangedAtoATA


NASBAAstepsA-AAnswer✓A1.AHybridizeAoligo-T7PAprimerAtoAtargetAseq

2.ART/RNaseAH
3.AHybridizeAwithAtarget-specificAoligoAprimerA(P2)
4.ARNAAtranscriptAofAT7ARNAApol


DoAyouAhaveAtoAknowAtheAgeneAsequenceAinAorderAtoAdoADNAAsequencing?A-
AAnswer✓AYes,AinAorderAtoAdesignAprimers

YouAdoANOTAneedAtoAknowAtheAmutation


SangerAsequencingAmethodA-AAnswer✓ADividedAintoA4AsamplesA(ddA,AT,AG,AC)
LabelAwithAradioactive/dyeAoligoAatA3'Aend
MixAwithAtaq,AdNTPs,AddNTPAandAincubate
runAonAgelA-->AfragsAwillAterminateAatAdifferentAlengths


FluorescentAinAsituAhybridizationA(FISH)A-
AAnswer✓AUsesAfluorescentAprobesAtoAdetectADNAAsequencesAonAchr