ASCP CG Certification Practice
Final Exam Questions Comprehensive Resource To Help
You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100%
Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. What is used to reduce mycoplasma contamination?
- Correct Answer: BM cycline
2. CLIA '88 stipulates that equipment must be recalibrated ____ ?
- Correct Answer: After a major repair to ensure still operational
3. How should caustic and corrosive chemicals be stored?
- Correct Answer: Both should be stored in a nonflammable cabinet
4. What factors would contribute to ALL cultures failing in a CO2 incubator? -
Correct Answer: Temperature
CO2 level
Humidity level
5. What is the modal number for a diploid karyotype and an additional CBG
negative marker chromosome? - Correct Answer: 46
CBG = c-bands achieved with barium with Giemsa stain
, CBG negative referrs to not having a centromere, which is crucial to being
included in the modal number, so this extra fragment does not have a
centromere and is not counted towards the total
6. What is included when recording the modal number?
- Correct Answer: Mar - a marker chromosome is included because it
has a centromere
7. What is the BEST way to avoid producing ghost-like, pale chromosomes
while staining? - Correct Answer: Decrease trypsin time
8. In order to ensure successful harvest, each new batch of hypotonic solution
should be evaluated ____. - Correct Answer: By comparing new
hypotonic solution to that already in use
9. Which cell type would you want to use for long term serial cytogenetic
studies? - Correct Answer: Fibroblast cells
10.What is not a necessary (must be added) ingredient which is added to
modified medium? - Correct Answer: Antibiotics
11.What chemical is used for cryopreservation? - Correct Answer: Dimethyl
sulfoxide (DMSO)
,12.You are analyzing a clinical case and discover a small extra marker with
satellites. What staining technique would be most useful in determining the
chromosomal origin of this marker? - Correct Answer: NOR
13.When using a fluorescein dye (yellow-green) labeled probe during the FISH
procedure, which counterstain should be used? - Correct Answer:
Propidium iodide (red)
14.A bone marrow sample collected in transport medium is leaking into the
sealed plastic bag in which it was transported. The technologist
should_______. - Correct Answer: Clean up the tube while wearing
gloves
15.A laboratory' quality assurance program includes _______________. -
Correct Answer: Proficiency testing
16.All of the following chromosomes have dark bands on chromosome regions
that can be used as indicators when preparing the best image: - Correct
Answer: Chromosome 11p
Chromosome 12q
Chromosome 17q
17.The morning after being set-up, a single dish of an amniotic fluid culture
turns cloudy and appears contaminated. None of the other cultures set-up
that day appears contaminated. What should be done first? - Correct
Answer: Harvest the culture as soon as possible
, 18.When the addition of nonhomologous material to the q-arm of
chromosome 16 is suspected after G-banding, what banding technique
should be used? - Correct Answer: C-banding
19.What chromosome is most different between C- and G-banding? -
Correct Answer: Chromosome 9
20.What chromosome region does quinacrine and acridine orange dyes stain? -
Correct Answer: AT rich DNA
21.Under an electron microscope, what is observed as a consequence of all
banding procedures? - Correct Answer: Chromosomal collapse
22.What is the best agent to use against mycoplasma? - Correct Answer:
BM cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
23.For successful harvest, each new batch of hypotonic solution should be
evaluated by: - Correct Answer: comparing the new lot to a lot already
in use
24.Colagenase, pronase, and trypsin are: - Correct Answer: Proteolytic
enzymes - break down tissues into single-cell suspensions
Final Exam Questions Comprehensive Resource To Help
You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100%
Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. What is used to reduce mycoplasma contamination?
- Correct Answer: BM cycline
2. CLIA '88 stipulates that equipment must be recalibrated ____ ?
- Correct Answer: After a major repair to ensure still operational
3. How should caustic and corrosive chemicals be stored?
- Correct Answer: Both should be stored in a nonflammable cabinet
4. What factors would contribute to ALL cultures failing in a CO2 incubator? -
Correct Answer: Temperature
CO2 level
Humidity level
5. What is the modal number for a diploid karyotype and an additional CBG
negative marker chromosome? - Correct Answer: 46
CBG = c-bands achieved with barium with Giemsa stain
, CBG negative referrs to not having a centromere, which is crucial to being
included in the modal number, so this extra fragment does not have a
centromere and is not counted towards the total
6. What is included when recording the modal number?
- Correct Answer: Mar - a marker chromosome is included because it
has a centromere
7. What is the BEST way to avoid producing ghost-like, pale chromosomes
while staining? - Correct Answer: Decrease trypsin time
8. In order to ensure successful harvest, each new batch of hypotonic solution
should be evaluated ____. - Correct Answer: By comparing new
hypotonic solution to that already in use
9. Which cell type would you want to use for long term serial cytogenetic
studies? - Correct Answer: Fibroblast cells
10.What is not a necessary (must be added) ingredient which is added to
modified medium? - Correct Answer: Antibiotics
11.What chemical is used for cryopreservation? - Correct Answer: Dimethyl
sulfoxide (DMSO)
,12.You are analyzing a clinical case and discover a small extra marker with
satellites. What staining technique would be most useful in determining the
chromosomal origin of this marker? - Correct Answer: NOR
13.When using a fluorescein dye (yellow-green) labeled probe during the FISH
procedure, which counterstain should be used? - Correct Answer:
Propidium iodide (red)
14.A bone marrow sample collected in transport medium is leaking into the
sealed plastic bag in which it was transported. The technologist
should_______. - Correct Answer: Clean up the tube while wearing
gloves
15.A laboratory' quality assurance program includes _______________. -
Correct Answer: Proficiency testing
16.All of the following chromosomes have dark bands on chromosome regions
that can be used as indicators when preparing the best image: - Correct
Answer: Chromosome 11p
Chromosome 12q
Chromosome 17q
17.The morning after being set-up, a single dish of an amniotic fluid culture
turns cloudy and appears contaminated. None of the other cultures set-up
that day appears contaminated. What should be done first? - Correct
Answer: Harvest the culture as soon as possible
, 18.When the addition of nonhomologous material to the q-arm of
chromosome 16 is suspected after G-banding, what banding technique
should be used? - Correct Answer: C-banding
19.What chromosome is most different between C- and G-banding? -
Correct Answer: Chromosome 9
20.What chromosome region does quinacrine and acridine orange dyes stain? -
Correct Answer: AT rich DNA
21.Under an electron microscope, what is observed as a consequence of all
banding procedures? - Correct Answer: Chromosomal collapse
22.What is the best agent to use against mycoplasma? - Correct Answer:
BM cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
23.For successful harvest, each new batch of hypotonic solution should be
evaluated by: - Correct Answer: comparing the new lot to a lot already
in use
24.Colagenase, pronase, and trypsin are: - Correct Answer: Proteolytic
enzymes - break down tissues into single-cell suspensions
