ASCP CG (Classic Cytogenetics) Certification
Final Exam Questions Comprehensive Resource To Help
You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100%
Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. Collagenase is used in culture of solid tissues to?
a. Dissociate cells
b. To supplement culture media
c. To prevent mycoplasma contamination
d. To subculture the monolayer cultures - Correct Answer: Dissociate cells
2. What microscope is useful in the separation of fetal tissue from maternal tissue?
a. Brightfield microscope
b. Fluorescent microscope
c. Compound microscope
d. Stereo (dissecting) - microscope - Correct Answer: Stereo (dissecting) -
microscope
3. Which of the following should be added to modified media before use in amniotic fluid
cultures?
a. Fetal Bovine Serum (FBS)
b. Vitamins
c. Sucrose
d. Gentamycin - Correct Answer: Fetal Bovine Serum (FBS)
4. Which the following is often added to media, salt solutions, and enzymes as a pH
indicator?
a. Sodium bicarbonate
b. CO2
c. Hepes buffer
, d. Phenol red - Correct Answer: Phenol red
5. Which of the following peripheral blood cultures stimulated with PHA can be used for
chromosome preparations?
a. 48 hr
b. 72 hr
c. 92 hr
d. All of the above - Correct Answer: All of the above
6. Which of the following biological safety cabinets will allow microbial contamination of
the specimen if used during tissue culture?
a. Horizontal laminar flow hood
b. Biological safety cabinets Class 1
c. Biological safety cabinets Class 2
d. Biological safety cabinets Class 3 - Correct Answer: Biological safety cabinets
Class 1
7. To prevent cross contamination of cultures, a tissue culturist SHOULD?
a. Use the same media bottle for long term and primary cultures.
b. Work with a transformed culture at the same time in the culture hood as a
primary culture.
c. Change the pipette after feeding cultures from different patients.
d. Touch the lip of the media bottle against the top of the flask being fed. -
Correct Answer: Change the pipette after feeding cultures from different
patients.
8. When subculturing a long-term cell line, a technician finds that the pH of the media has
turned a yellow color overnight. When looked at through the inverted microscope many
small dot structures can be seen around the cells. Which of the following may be true
about this culture?
a. The medium has turned yellow because these cells are dividing very rapidly and
need to be sub-cultured.
b. The acidic media and dot-like structures are the result of cell death due to
overgrowth of the cell culture.
, c. This culture is contaminated with a microbe which is making the media acidic.
d. This is a normal phenomenon observed in cell culturing. - Correct Answer:
This culture is contaminated with a microbe which is making the media acidic.
9. The inverted microscope is essential in the monitoring of cells cultures by allowing the
technologist to visualize all but which of the following cellular features?
a. Cellular confluency
b. Chromosomal abnormalities
c. Mitotic cells
d. Cellular attachment - Correct Answer: Chromosomal abnormalities
10. Amniotic fluid cultures grown in the flask method are considered to be ready for
harvesting when they are?
a. 50% confluent
b. 75% confluent
c. 90% confluent
d. 100% confluent - Correct Answer: 75% confluent
11. In order to store cells for later study experiments, scientists will cryopreserve cells at
extremely low temperature (-90 'C to 195 'C). What is the function of the freezing
solution?
a. This solution dehydrates the cell
b. This solution provides nutrients
c. This solution ruptures the cell membrane
d. This solution will slow cell metabolism - Correct Answer: This solution
dehydrates the cell
12. Cell death in a peripheral blood culture may be the result of?
a. A five percent CO2 atmosphere
b. a pH of 7.2
c. a 24 hr delay in culturing
d. Incubation at a temp of 40 'C - Correct Answer: Incubation at a temp of 40 'C
Final Exam Questions Comprehensive Resource To Help
You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100%
Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. Collagenase is used in culture of solid tissues to?
a. Dissociate cells
b. To supplement culture media
c. To prevent mycoplasma contamination
d. To subculture the monolayer cultures - Correct Answer: Dissociate cells
2. What microscope is useful in the separation of fetal tissue from maternal tissue?
a. Brightfield microscope
b. Fluorescent microscope
c. Compound microscope
d. Stereo (dissecting) - microscope - Correct Answer: Stereo (dissecting) -
microscope
3. Which of the following should be added to modified media before use in amniotic fluid
cultures?
a. Fetal Bovine Serum (FBS)
b. Vitamins
c. Sucrose
d. Gentamycin - Correct Answer: Fetal Bovine Serum (FBS)
4. Which the following is often added to media, salt solutions, and enzymes as a pH
indicator?
a. Sodium bicarbonate
b. CO2
c. Hepes buffer
, d. Phenol red - Correct Answer: Phenol red
5. Which of the following peripheral blood cultures stimulated with PHA can be used for
chromosome preparations?
a. 48 hr
b. 72 hr
c. 92 hr
d. All of the above - Correct Answer: All of the above
6. Which of the following biological safety cabinets will allow microbial contamination of
the specimen if used during tissue culture?
a. Horizontal laminar flow hood
b. Biological safety cabinets Class 1
c. Biological safety cabinets Class 2
d. Biological safety cabinets Class 3 - Correct Answer: Biological safety cabinets
Class 1
7. To prevent cross contamination of cultures, a tissue culturist SHOULD?
a. Use the same media bottle for long term and primary cultures.
b. Work with a transformed culture at the same time in the culture hood as a
primary culture.
c. Change the pipette after feeding cultures from different patients.
d. Touch the lip of the media bottle against the top of the flask being fed. -
Correct Answer: Change the pipette after feeding cultures from different
patients.
8. When subculturing a long-term cell line, a technician finds that the pH of the media has
turned a yellow color overnight. When looked at through the inverted microscope many
small dot structures can be seen around the cells. Which of the following may be true
about this culture?
a. The medium has turned yellow because these cells are dividing very rapidly and
need to be sub-cultured.
b. The acidic media and dot-like structures are the result of cell death due to
overgrowth of the cell culture.
, c. This culture is contaminated with a microbe which is making the media acidic.
d. This is a normal phenomenon observed in cell culturing. - Correct Answer:
This culture is contaminated with a microbe which is making the media acidic.
9. The inverted microscope is essential in the monitoring of cells cultures by allowing the
technologist to visualize all but which of the following cellular features?
a. Cellular confluency
b. Chromosomal abnormalities
c. Mitotic cells
d. Cellular attachment - Correct Answer: Chromosomal abnormalities
10. Amniotic fluid cultures grown in the flask method are considered to be ready for
harvesting when they are?
a. 50% confluent
b. 75% confluent
c. 90% confluent
d. 100% confluent - Correct Answer: 75% confluent
11. In order to store cells for later study experiments, scientists will cryopreserve cells at
extremely low temperature (-90 'C to 195 'C). What is the function of the freezing
solution?
a. This solution dehydrates the cell
b. This solution provides nutrients
c. This solution ruptures the cell membrane
d. This solution will slow cell metabolism - Correct Answer: This solution
dehydrates the cell
12. Cell death in a peripheral blood culture may be the result of?
a. A five percent CO2 atmosphere
b. a pH of 7.2
c. a 24 hr delay in culturing
d. Incubation at a temp of 40 'C - Correct Answer: Incubation at a temp of 40 'C
