ASCP CG (Classic Cytogenetics) Certification
Final Exam Questions Comprehensive Resource To Help
You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100%
Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. What are the two classes of lymphocytes? - Correct Answer: T-cells and
B-cells.
2. What mitogens do T-cells respond to? - Correct Answer: PHA and
Concanavalin A.
3. What mitogens do B-cells respond to? - Correct Answer: LPS, Epstein-
Barr Virus, and TPA.
4. Which mitogen stimulates both T and B cells? - Correct Answer:
Pokeweed.
5. What group of mitogens is used to stimulate cells with a constitutional
abnormality? - Correct Answer: T-cell mitogens.
6. How do we stimulate the growth of neoplastic cells or B-cells neoplasms? -
Correct Answer: Run one unstimulated culture and one with a B-cell
mitogen.
,7. How are percutaneous umbilical blood samples (PUBS) retrieved? -
Correct Answer: Transabdominally with the assistance of ultrasonography.
8. What is the turnaround time for an amniocentesis culture? - Correct
Answer: 7-14 days.
9. What is the turnaround time for a fetal blood culture? - Correct Answer:
2-3 days.
10.What is the earliest a PUB can be performed? - Correct Answer: 18
weeks.
11.When was amniocentesis first reported? - Correct Answer: 1880s
12.What did Steele and Breg do in 1966? - Correct Answer: They used
amniocentesis for cytogenetic analysis.
13.What is the risk for maternal cell contamination? - Correct Answer:
0.3% risk.
14.What is maternal cell contamination mostly attributed to? - Correct
Answer: Failure to discard the 2 cc of amniotic fluid and generally bloody
samples.
,15.What is the ideal gestational age for a specimen tap? - Correct Answer:
16-20 weeks.
16.Why are early and late specimen taps not ideal for culture? - Correct
Answer: The cultures will be slow growing.
17.What do we do with the remaining amniotic fluid that is not used for
culturing cells? - Correct Answer: We send it to another department for
AFP testing.
18.Which takes longer to grow in a culture: solid tissue or bone marrow? -
Correct Answer: Solid tissue.
19.What is the most common issue with solid tissue culture? - Correct
Answer: Microbial contamination?
20.What did Hahnemann and Mohr develop in 1968? - Correct Answer:
Chorionic Villus Sampling (CVS).
21.How are CVS samples retrieved? - Correct Answer: Transcervically and
transabdominally.
22.How do we reduce cellular contamination of a chorionic villus sample? -
Correct Answer: By removing the adherent maternal decidua.
, 23.Describe the physical differences between villi and decidua. - Correct
Answer: The villi will be branched in appearance and lighter as compared to
the decidua, which will be more sheet-like and darker.
24.What is the prerequisite for using solid tissues in cytogenetic study? -
Correct Answer: That the cells have the ability to divide.
25.Why do we remove necrotic tissue and normal parenchyma from the tissue
before setting a culture? - Correct Answer: Necrotic tissue increases the
toxicity of the culture, and normal cells will typically outgrow the abnormal
cells in this environment.
26.What fluids can we use to study metastatic cells? - Correct Answer:
Pleural and abdominal ascites fluids.
27.What is the process in which peripheral stem cells are harvested? -
Correct Answer: Apheresis. Blood is removed and run through a machine
that removes stem cells. The rest of the blood is returned to the patient.
28.Why do we study stem cells? - Correct Answer: Stem cells are capable
of reproducing themselves and generating other cells that evolve into
WBCs, RBCs, and platelets.
29.What do we inspect upon receiving a sample? - Correct Answer:
Appearance, volume, collection container, transport time, and transport
conditions.
Final Exam Questions Comprehensive Resource To Help
You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100%
Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. What are the two classes of lymphocytes? - Correct Answer: T-cells and
B-cells.
2. What mitogens do T-cells respond to? - Correct Answer: PHA and
Concanavalin A.
3. What mitogens do B-cells respond to? - Correct Answer: LPS, Epstein-
Barr Virus, and TPA.
4. Which mitogen stimulates both T and B cells? - Correct Answer:
Pokeweed.
5. What group of mitogens is used to stimulate cells with a constitutional
abnormality? - Correct Answer: T-cell mitogens.
6. How do we stimulate the growth of neoplastic cells or B-cells neoplasms? -
Correct Answer: Run one unstimulated culture and one with a B-cell
mitogen.
,7. How are percutaneous umbilical blood samples (PUBS) retrieved? -
Correct Answer: Transabdominally with the assistance of ultrasonography.
8. What is the turnaround time for an amniocentesis culture? - Correct
Answer: 7-14 days.
9. What is the turnaround time for a fetal blood culture? - Correct Answer:
2-3 days.
10.What is the earliest a PUB can be performed? - Correct Answer: 18
weeks.
11.When was amniocentesis first reported? - Correct Answer: 1880s
12.What did Steele and Breg do in 1966? - Correct Answer: They used
amniocentesis for cytogenetic analysis.
13.What is the risk for maternal cell contamination? - Correct Answer:
0.3% risk.
14.What is maternal cell contamination mostly attributed to? - Correct
Answer: Failure to discard the 2 cc of amniotic fluid and generally bloody
samples.
,15.What is the ideal gestational age for a specimen tap? - Correct Answer:
16-20 weeks.
16.Why are early and late specimen taps not ideal for culture? - Correct
Answer: The cultures will be slow growing.
17.What do we do with the remaining amniotic fluid that is not used for
culturing cells? - Correct Answer: We send it to another department for
AFP testing.
18.Which takes longer to grow in a culture: solid tissue or bone marrow? -
Correct Answer: Solid tissue.
19.What is the most common issue with solid tissue culture? - Correct
Answer: Microbial contamination?
20.What did Hahnemann and Mohr develop in 1968? - Correct Answer:
Chorionic Villus Sampling (CVS).
21.How are CVS samples retrieved? - Correct Answer: Transcervically and
transabdominally.
22.How do we reduce cellular contamination of a chorionic villus sample? -
Correct Answer: By removing the adherent maternal decidua.
, 23.Describe the physical differences between villi and decidua. - Correct
Answer: The villi will be branched in appearance and lighter as compared to
the decidua, which will be more sheet-like and darker.
24.What is the prerequisite for using solid tissues in cytogenetic study? -
Correct Answer: That the cells have the ability to divide.
25.Why do we remove necrotic tissue and normal parenchyma from the tissue
before setting a culture? - Correct Answer: Necrotic tissue increases the
toxicity of the culture, and normal cells will typically outgrow the abnormal
cells in this environment.
26.What fluids can we use to study metastatic cells? - Correct Answer:
Pleural and abdominal ascites fluids.
27.What is the process in which peripheral stem cells are harvested? -
Correct Answer: Apheresis. Blood is removed and run through a machine
that removes stem cells. The rest of the blood is returned to the patient.
28.Why do we study stem cells? - Correct Answer: Stem cells are capable
of reproducing themselves and generating other cells that evolve into
WBCs, RBCs, and platelets.
29.What do we inspect upon receiving a sample? - Correct Answer:
Appearance, volume, collection container, transport time, and transport
conditions.
