Practice Questions Test Bank 2 with Solutions
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What is a transgenic mouse?
a. A mouse that has DNA from a different source in its genes.
b. A mouse that had DNA removed or disabled in its genes.
c. A mouse that differs from another strain by one gene.
d. A mouse with the genetic makeup of two strains. - Correct Answer-a. A mouse that has DNA
from a different source in its genes.
What does it mean if an animal is considered to be a wild type animal?
a. A new gene has been inserted in the animal.
b. A gene has been turned off in the animal.
c. The animal does not contain the allele of interest.
d. The animal has homozygous alleles for the gene of interest. - Correct Answer-c. The animal
does not contain the allele of interest.
What is the name of the technique for injecting DNA directly into the fertilized egg with a tiny
glass pipette?
a. Intraembryonic injection
b. Retroviral insertion
c. Embryonic stem cell insertion
d. Pronuclear injection - Correct Answer-d. Pronuclear injection
Which technique collects the embryonic stem cells that line the inside of a developing
blastocyst to produce embryonic stem cell lines?
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,a. Pronuclear injection
b. Genotyping
c. Homologous recombination
d. None of the above - Correct Answer-c. Homologous recombination
What process occurs during electroporation?
a. Electricity passes through an embryo so that the pipette containing DNA can enter the cell.
b. Electricity passes through a DNA + virus solution causing the DNA to become incorporated
into the virus.
c. Electricity passes through a DNA + stem cell solution causing the DNA to enter the cells.
d. Electricity passes through a stem cell solution after DNA has entered the cell to seal the cell. -
Correct Answer-c. Electricity passes through a DNA + stem cell solution causing the DNA to
enter the cells.
What is a chimera?
a. An individual who has homozygous alleles for a specific gene
b. An individual who has some cells containing the new gene and some cells without the new
gene
c. An individual who has a gene absent or turned off due to a mutation in all its cells
d. An individual who has both copies of a gene absent or turned off - Correct Answer-b. An
individual who has some cells containing the new gene and some cells without the new gene
What is it called when a new gene is incorporated into the germ cells (egg and sperm)?
a. Genotyping
b. Pronuclear injection
c. Germline transmission
d. Polymerase chain reaction - Correct Answer-c. Germline transmission
What is the difference between genotype and phenotype?
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,a. The genotype is the genetic makeup; the phenotype is how genes are expressed.
b. The phenotype is the genetic makeup; the genotype is how genes are expressed.
c. Genotype is a visible expression of the genes; phenotype is a behavioral expression.
d. Genotype is the wild type; phenotype is the mutant form. - Correct Answer-a. The genotype
is the genetic makeup; the phenotype is how genes are expressed.
Excessive fear is an example of which phenotype?
a. Visible phenotype
b. Behavioral phenotype
c. Inducible phenotype
d. Physiological phenotype - Correct Answer-b. Behavioral phenotype
What is the term that refers to genes that are located adjacent to one another on the same
chromosome that tend to be inherited together?
a. Inheritance
b. Gene linkage
c. Mutation
d. Transgenic - Correct Answer-b. Gene linkage
Which part of the strain name is the maternal strain for the B6C3F1/Crl mouse?
a. B6
b. C3
c. F1
d. Crl - Correct Answer-a. B6
What type of mating occurs when only 1 female and 1 male are put together in a cage?
a. Monogamous mating
b. Polygamous mating
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, c. Harem mating
d. Outbreeding - Correct Answer-a. Monogamous mating
What advantage does using a timed mating system provide?
a. It helps avoid fighting among the cage mates.
b. It can provide embryos or mice at a precise gestational age needed for experiments.
c. It reduces the number of animals needed for breeding.
d. It is less expensive than other systems. - Correct Answer-b. It can provide embryos or mice at
a precise gestational age needed for experiments.
What is the Whitten Effect?
a. A male is added to a cage of females, resulting in the synchronization of their estrous cycles.
b. A female is paired to a younger adult male, resulting in more offspring.
c. A litter of pups that is born during the night will be more likely to survive.
d. Postpartum estrus occurs after a female gives birth - Correct Answer-a. A male is added to a
cage of females, resulting in the synchronization of their estrous cycles.
What characteristic results from breeding outbred stocks breeding?
a. Homogeneity
b. Maximum mutations
c. Minimal hybrids
d. Genetic variation - Correct Answer-d. Genetic variation
How many generations of brother-to-sister matings are needed to establish an inbred strain?
a. A minimum of 5
b. A minimum of 10
c. A minimum of 20
d. A minimum of 30 - Correct Answer-c. A minimum of 20
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