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The flow of genetic information: DNA → RNA →
Central Dogma
Protein.
The process of making RNA from DNA,
Transcription
occurring in the nucleus.
The process of making protein from RNA,
Translation
occurring at ribosomes in the cytoplasm.
A technique that separates DNA fragments by
Gel Electrophoresis
size using electricity.
A sterilization method using high pressure
Autoclaving
steam at 121°C for 15-30 minutes.
Luria-Bertani medium used for bacterial growth
LB Media containing tryptone, yeast extract, NaCl, and
agar.
Laboratory tools used to measure and transfer
Micropipettes
small volumes of liquid.
A technique to amplify specific DNA sequences
PCR (Polymerase Chain Reaction)
exponentially.
Heat-stable enzyme used in PCR that
Taq Polymerase
synthesizes new DNA strands.
The process of joining two DNA fragments
Ligation
together using DNA ligase.
The introduction of plasmid DNA into bacterial
Transformation
cells.