BACTERIAL IDENTIFICATION VIRTUAL
LAB EXAM QUESTIONS AND ANSWERS
(VERIFIED AND UPDATED)
What do the green and blue tubes contain. - ANS Consists of buffers, primers, DNA
polymerase, nucleotides and fluorescence-tagged terminators in suitable proportions
The purpose of the second PCR is not to create identical copies like the first PCR you ran. What
is the purpose of this PCR? - ANS Produce many copies of variable length
Where do scientists obtain primers to be used in PCR and in this technique? - ANS Each DNA
strand binds primer @ one end and will have a fluorescence- tagged terminator @ one end
called PCR amplification
What's the final PCR product, the stuff contained in your 12 tubes? - ANS Nice of DNA pieces
of variable length
What's the purpose of gel electrophoresis? - ANS Method to separate molecules based in
differences in size
How do DNA molecules move in relation to charge? Why? - ANS DNA is negative charge and
the syringe end has positive charge therefore the DNA moves to the positive end since opposite
charges attract
@2026/2027 ALLRIGHTS RESERVED.
LAB EXAM QUESTIONS AND ANSWERS
(VERIFIED AND UPDATED)
What do the green and blue tubes contain. - ANS Consists of buffers, primers, DNA
polymerase, nucleotides and fluorescence-tagged terminators in suitable proportions
The purpose of the second PCR is not to create identical copies like the first PCR you ran. What
is the purpose of this PCR? - ANS Produce many copies of variable length
Where do scientists obtain primers to be used in PCR and in this technique? - ANS Each DNA
strand binds primer @ one end and will have a fluorescence- tagged terminator @ one end
called PCR amplification
What's the final PCR product, the stuff contained in your 12 tubes? - ANS Nice of DNA pieces
of variable length
What's the purpose of gel electrophoresis? - ANS Method to separate molecules based in
differences in size
How do DNA molecules move in relation to charge? Why? - ANS DNA is negative charge and
the syringe end has positive charge therefore the DNA moves to the positive end since opposite
charges attract
@2026/2027 ALLRIGHTS RESERVED.
