MCB3023 EXAM 1 MODULE 1.3/4
100% CORRECT
Several Archaea have a lipid monolayer instead of a lipid bilayer comprising their
cytoplasmic membrane. Why could this be an advantage? - ANSWER-more stable at
high temps
the archaeal chromosome is: - ANSWER-circular and condensed by histones
chaperone proteins in hyperthermophilic archaeal cells are known are - ANSWER-
thermosomes
virus concentration requires which of the following techniques - ANSWER-plague
assay
in reverse-transcriptase PCR - ANSWER-RNA to DNA, then amplify with PCR
what would you use to measure the optical density of a bacteria culture - ANSWER-
spectrophotometer
which medium is used to allow some microbes on a plate to be distinguished from
others? - ANSWER-differential
which medium is used to allow slow-growing microbes to gain a competitive growth
advantage? - ANSWER-enrichment
in order for a chemostat to operate properly, what must the reservoir contain -
ANSWER-a limiting nutrient
how long would it take a culture of E.coli with a starting cell density of 1 X 105 cells/
ml to reach a cell density of 1 X 108 cells/ml if it had a generation time of 30
minutes? - ANSWER-5 hours
which of these is one of the main functions of the cytoplasmic membrane in
Archaea? - ANSWER-it acts as a semi permeable barrier to the diffusion of
molecules into and out of the cell
what is the primary reason that Woese and Fox chose the rRNA molecule to study
phylogenetic relationships? - ANSWER-ribosomal RNA is a molecule found in all
living organisms
which of the following is NOT characteristic of the chromosome found in most
archaeal cells - ANSWER-the chromosome is linear
which of these best describes the process of meiosis in eukaryal cells - ANSWER-
DNA replication followed by two cell divisions
, all of the following methods can be used to quantify viruses EXCEPT: - ANSWER-
viral protein assay
which of these methods can be used to obtain a viable cell count - ANSWER-plate
counts of serial dilution
some archaeal cells contain pseudopeptidoglycan as their major cell wall
component. what is this material composed of? - ANSWER-sugar polymers cross-
linked via a peptide bridge
what is the size range of a typical archaea cell - ANSWER-1-5um
all known hyperthermophiles possess this enzyme to increase the supercoiling of the
DNA and help stabilize it at high temps - ANSWER-reverse DNA gyrase
many of the hyperthermophiles crenarchaeotes that have been grown in culture are
also - ANSWER-acidophiles
how do the bacteria and achaea differ in the way the flagellum proteins are handled -
ANSWER-archaea flagellum proteins are added to the growing flagellum shaft at the
base
eukaryal proteins that are secreted outside of the cell undergo modification before
secretion. which organelles are involved in this secretion pathway? - ANSWER-the
endoplasmic reticulum and golgi apparatus
Haploid Dictyostelium replicate by mitosis when nutrients are plentiful. What
happens when growth conditions deteriorate? - ANSWER-the cells aggregate to
form a multicellular slug
the enzyme catalyst is used to detoxify which harmful oxygen species - ANSWER-
hydrogen peroxide
which of these is a characteristic of a halophile - ANSWER-it grows only in the
presence of high amounts of salt
how many phyla found in the archaea domain are well-characterized? - ANSWER-2
all mitochondria contain DNA that shared sequence similarity with bacteria from the
phylum _____ - ANSWER-proteobacteria
a protein produced in the rough endoplasmic reticulum has the amino acid sequence
KKKRK in a central position. what is the destination of this protein? - ANSWER-
nucleus
how do bacteriophages invade the bacterial cell? - ANSWER-the bacteriophage
directly injects its nucleic acid
in which phase of the growth curve does preparation for cell division and synthesis of
enzymes needed for catabolic activity take place? - ANSWER-lag phase
100% CORRECT
Several Archaea have a lipid monolayer instead of a lipid bilayer comprising their
cytoplasmic membrane. Why could this be an advantage? - ANSWER-more stable at
high temps
the archaeal chromosome is: - ANSWER-circular and condensed by histones
chaperone proteins in hyperthermophilic archaeal cells are known are - ANSWER-
thermosomes
virus concentration requires which of the following techniques - ANSWER-plague
assay
in reverse-transcriptase PCR - ANSWER-RNA to DNA, then amplify with PCR
what would you use to measure the optical density of a bacteria culture - ANSWER-
spectrophotometer
which medium is used to allow some microbes on a plate to be distinguished from
others? - ANSWER-differential
which medium is used to allow slow-growing microbes to gain a competitive growth
advantage? - ANSWER-enrichment
in order for a chemostat to operate properly, what must the reservoir contain -
ANSWER-a limiting nutrient
how long would it take a culture of E.coli with a starting cell density of 1 X 105 cells/
ml to reach a cell density of 1 X 108 cells/ml if it had a generation time of 30
minutes? - ANSWER-5 hours
which of these is one of the main functions of the cytoplasmic membrane in
Archaea? - ANSWER-it acts as a semi permeable barrier to the diffusion of
molecules into and out of the cell
what is the primary reason that Woese and Fox chose the rRNA molecule to study
phylogenetic relationships? - ANSWER-ribosomal RNA is a molecule found in all
living organisms
which of the following is NOT characteristic of the chromosome found in most
archaeal cells - ANSWER-the chromosome is linear
which of these best describes the process of meiosis in eukaryal cells - ANSWER-
DNA replication followed by two cell divisions
, all of the following methods can be used to quantify viruses EXCEPT: - ANSWER-
viral protein assay
which of these methods can be used to obtain a viable cell count - ANSWER-plate
counts of serial dilution
some archaeal cells contain pseudopeptidoglycan as their major cell wall
component. what is this material composed of? - ANSWER-sugar polymers cross-
linked via a peptide bridge
what is the size range of a typical archaea cell - ANSWER-1-5um
all known hyperthermophiles possess this enzyme to increase the supercoiling of the
DNA and help stabilize it at high temps - ANSWER-reverse DNA gyrase
many of the hyperthermophiles crenarchaeotes that have been grown in culture are
also - ANSWER-acidophiles
how do the bacteria and achaea differ in the way the flagellum proteins are handled -
ANSWER-archaea flagellum proteins are added to the growing flagellum shaft at the
base
eukaryal proteins that are secreted outside of the cell undergo modification before
secretion. which organelles are involved in this secretion pathway? - ANSWER-the
endoplasmic reticulum and golgi apparatus
Haploid Dictyostelium replicate by mitosis when nutrients are plentiful. What
happens when growth conditions deteriorate? - ANSWER-the cells aggregate to
form a multicellular slug
the enzyme catalyst is used to detoxify which harmful oxygen species - ANSWER-
hydrogen peroxide
which of these is a characteristic of a halophile - ANSWER-it grows only in the
presence of high amounts of salt
how many phyla found in the archaea domain are well-characterized? - ANSWER-2
all mitochondria contain DNA that shared sequence similarity with bacteria from the
phylum _____ - ANSWER-proteobacteria
a protein produced in the rough endoplasmic reticulum has the amino acid sequence
KKKRK in a central position. what is the destination of this protein? - ANSWER-
nucleus
how do bacteriophages invade the bacterial cell? - ANSWER-the bacteriophage
directly injects its nucleic acid
in which phase of the growth curve does preparation for cell division and synthesis of
enzymes needed for catabolic activity take place? - ANSWER-lag phase
