MLPAO Test Prep ~ Hematology || Verified for Accuracy.
What does CBC stand for? correct answers Complete Blood Count
What are the formed elements of blood? correct answers WBC, RBC, and Platelets
EDTA Anticoagulant correct answers Maintains cell shape and size and prevents blood from
clotting by binding to calcium
Heparin Anticoagulant correct answers Initiates cell clumping and provides blue background
staining
Citrate Anticoagulant correct answers Dilutes the blood due to the volume of the liquid and is
used for platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing correct answers 4
CBC handling and storage: When transporting to other labs/hospitals correct answers surround
with ice packs
CBC handling and storage: Samples are _____ before testing correct answers mixed well by
inversion
CBC handling and storage: Samples must be tested within __ correct answers 24h
CBC handling and storage: Peripheral blood smears must be made within correct answers 24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED FOR
TESTING correct answers 24h
,What happens if CBC is tested after 24h? correct answers Platelets clump, WBC nuclei
disintegrate, WBC cytoplasm contain dark granules and Vacuoles, and RBCs swell and break
down
Coagulation: Specimen of choice? correct answers Plasma
Coagulation: What colour tube? What is the additive? correct answers light blue, sodium citrate
Sodium Citrate binds to ___ preventing ____ correct answers calcium, coagulation
Sodium Citrate tube must be correct answers filled to the maximum draw capacity
Coagulation: Document if patient on correct answers anticoagulant therapy or aspirin
Handling and Storage of Coagulation samples: Preparation requirements correct answers
Centrifuge and separate
Handling and Storage of Coagulation samples: ____ plasma if not tested within ___ of arrival
correct answers Freeze, 4h
Coagulation samples: Some clotting factors are considered to be correct answers highly labile
(destroyed at room temperature)
What are the three methods of Peripheral blood smears (PBS)? correct answers Wedge method
(manual), Spun method (automated slide spinner), and automated method (analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader slide
___ correct answers Decreased
, Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered. correct
answers First
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader slide
___ correct answers Increased
HCT Values: Male, Female correct answers 0.40-0.50, 0.35-0.59
Good blood film should be ___ to ___ the length of the slide correct answers 2/3, ¾
Good blood film should have a slightly ___ or ____ edge correct answers Rounded, feathered
The angle of the spreader slide is approximately ___ degrees correct answers 35
The greater the angle of the spreader slide the ____ and _____ the film correct answers Shorter,
Thicker
The lower the angle of the spreader slide the ___ and ____ the film correct answers Longer and
Thinner
Blood film should be allowed to ____ ___ completely before staining procedures correct answers
air dry
Fixative found in stain is correct answers Methanol
Slides are washed with buffer of pH ___ to develop colour on the slide correct answers 6.4
Stain is comprised of ____ and ____ dyes correct answers Basic, acidic
What does CBC stand for? correct answers Complete Blood Count
What are the formed elements of blood? correct answers WBC, RBC, and Platelets
EDTA Anticoagulant correct answers Maintains cell shape and size and prevents blood from
clotting by binding to calcium
Heparin Anticoagulant correct answers Initiates cell clumping and provides blue background
staining
Citrate Anticoagulant correct answers Dilutes the blood due to the volume of the liquid and is
used for platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing correct answers 4
CBC handling and storage: When transporting to other labs/hospitals correct answers surround
with ice packs
CBC handling and storage: Samples are _____ before testing correct answers mixed well by
inversion
CBC handling and storage: Samples must be tested within __ correct answers 24h
CBC handling and storage: Peripheral blood smears must be made within correct answers 24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED FOR
TESTING correct answers 24h
,What happens if CBC is tested after 24h? correct answers Platelets clump, WBC nuclei
disintegrate, WBC cytoplasm contain dark granules and Vacuoles, and RBCs swell and break
down
Coagulation: Specimen of choice? correct answers Plasma
Coagulation: What colour tube? What is the additive? correct answers light blue, sodium citrate
Sodium Citrate binds to ___ preventing ____ correct answers calcium, coagulation
Sodium Citrate tube must be correct answers filled to the maximum draw capacity
Coagulation: Document if patient on correct answers anticoagulant therapy or aspirin
Handling and Storage of Coagulation samples: Preparation requirements correct answers
Centrifuge and separate
Handling and Storage of Coagulation samples: ____ plasma if not tested within ___ of arrival
correct answers Freeze, 4h
Coagulation samples: Some clotting factors are considered to be correct answers highly labile
(destroyed at room temperature)
What are the three methods of Peripheral blood smears (PBS)? correct answers Wedge method
(manual), Spun method (automated slide spinner), and automated method (analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader slide
___ correct answers Decreased
, Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered. correct
answers First
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader slide
___ correct answers Increased
HCT Values: Male, Female correct answers 0.40-0.50, 0.35-0.59
Good blood film should be ___ to ___ the length of the slide correct answers 2/3, ¾
Good blood film should have a slightly ___ or ____ edge correct answers Rounded, feathered
The angle of the spreader slide is approximately ___ degrees correct answers 35
The greater the angle of the spreader slide the ____ and _____ the film correct answers Shorter,
Thicker
The lower the angle of the spreader slide the ___ and ____ the film correct answers Longer and
Thinner
Blood film should be allowed to ____ ___ completely before staining procedures correct answers
air dry
Fixative found in stain is correct answers Methanol
Slides are washed with buffer of pH ___ to develop colour on the slide correct answers 6.4
Stain is comprised of ____ and ____ dyes correct answers Basic, acidic
