UAMS MICRO EXAM 3 Questions With
Correct Answers
OF |Reaction |for |Fermenters
Open |tube: |Acid |(yellow)
Closed |tube: |Acid |(yellow)
Ex: |Enterobacteriacea
OF |Reaction |for |Oxidizers
Open |tube: |Acid |(yellow)
Closed |tube: |No |acid |(green/blue)
Ex: |Pseudomonas |aeruginosa
OF |Reaction |for |Non-oxidizers
Open |tube: |No |acid |(green/blue)
Closed |tube: |No |acid |(green/blue)
Ex: |Assachrolytic |Acinetobacter
Nitrate |Reduction |Test:
Nitrate |reduced |to |Nitrite.
(+) |result; |red |color |within |30 |min |of |addition |of |Reagents |A |and |B.
Ex. |E. |coli.
Nitrate |Reduction |Test:
Nitrogen |gas |produced.
(+) |results. |Red |color |does |not |appear |with |addition |of |Reagents |A |and |B. |Medium |stays |
colorless |when |zinc |dust |is |added.
Ex: |Pseudomonas |aeruginosa.
,Nitrate |Reduction |Test:
Nitrate |not |reduced.
A |negative |result. |A |red |color |appears |after |the |addition |of |zinc |dust.
Ex: |Acinetobacter.
Pseudomonas |aeruginosa |Diseases
Causes |diseases |such |as |swimmer's |ear |and |infections |in |immunocompromised |patients |(CF |
and |burn |patients).
Pseudomonas |aeruginosa |Morphology
Morphology: |Large, |irregularly |shaped, |metallic |sheen. |Produces |Pyocyanin. |Beta |hemolytic |on
|sheep |BAP; |Grows |on |MAC |(lactose |neg).
Grows |on |routine |media |including |MAC.
P. |aeruginosa |Key |ID |Tests
Glucose |oxidizer
Oxidase |positive
Growth |at |42 |degrees
TSI: |A/NC
Produces |pyocyanin
Pseudomonas |aeruginosa
What |is |the |most |commonly |isolated |NFB?
Acinetobacter
What |is |the |2nd |most |commonly |isolated |NFB?
Which |species |of |Acinetobacter |are |saccharolytic |and |which |are |assacharolytic?
A. |baumannii |= |saccharolytic
A. |lwoffii |= |assaccharolytic
Acinetobacter |Key |ID |Tests
Oxidase |negative
Nitrate |negative
Glucose |utilization
Which |microbe |can |appear |as |pairs |of |plump |coccobacilli |or |as |filamentous |rods?
Acinetobacter
, What |is |the |3rd |most |commonly |isolated |NFB?
Stenotrophomonas |maltophilia
Key |ID |for |Stenotrophomonas |maltophilia
Oxidase |negative
Growth |on |MAC
Uses |glucose |and |maltose
Sensitive |to |SXT
Diseases |of |Burkholderia |cepacia
Important |nosocomial |pathogen
Cepacia |syndrome: |severe |resp |disease |in |CF |patients
What |color |is |B. |cepacia |on |BCSA |and |OFPBL |agar?
Yellow |- |produces |acid |from |lactose
What |microbe |has |a |strong, |earthy |odor?
Burkholderia |cepacia |(and |pseudomallei)
ID |for |B. |cepacia
Oxidase |positive |(weak)
Bipolar |staining |on |Gram |stain
What |microbe |causes |melioidosis, |an |aggressive |granulomatous |pulmonary |disease?
Burkholderia |pseudomallei
What |microbe |is |known |as |the |Vietnamese |time |bomb?
B. |pseudomallei
What |microbe |produces |wrinkled |colonies |on |lab |media?
B. |pseudomallei
ID |for |B. |pseudomallei
Oxidase |positive |(weak)
Moraxella |characteristics |on |Gram |stain
Pairs |of |plump |coccobacilli
Penicillin |test |is |used |to |differentiate |short |coccobacilli |to |true |cocci.
Correct Answers
OF |Reaction |for |Fermenters
Open |tube: |Acid |(yellow)
Closed |tube: |Acid |(yellow)
Ex: |Enterobacteriacea
OF |Reaction |for |Oxidizers
Open |tube: |Acid |(yellow)
Closed |tube: |No |acid |(green/blue)
Ex: |Pseudomonas |aeruginosa
OF |Reaction |for |Non-oxidizers
Open |tube: |No |acid |(green/blue)
Closed |tube: |No |acid |(green/blue)
Ex: |Assachrolytic |Acinetobacter
Nitrate |Reduction |Test:
Nitrate |reduced |to |Nitrite.
(+) |result; |red |color |within |30 |min |of |addition |of |Reagents |A |and |B.
Ex. |E. |coli.
Nitrate |Reduction |Test:
Nitrogen |gas |produced.
(+) |results. |Red |color |does |not |appear |with |addition |of |Reagents |A |and |B. |Medium |stays |
colorless |when |zinc |dust |is |added.
Ex: |Pseudomonas |aeruginosa.
,Nitrate |Reduction |Test:
Nitrate |not |reduced.
A |negative |result. |A |red |color |appears |after |the |addition |of |zinc |dust.
Ex: |Acinetobacter.
Pseudomonas |aeruginosa |Diseases
Causes |diseases |such |as |swimmer's |ear |and |infections |in |immunocompromised |patients |(CF |
and |burn |patients).
Pseudomonas |aeruginosa |Morphology
Morphology: |Large, |irregularly |shaped, |metallic |sheen. |Produces |Pyocyanin. |Beta |hemolytic |on
|sheep |BAP; |Grows |on |MAC |(lactose |neg).
Grows |on |routine |media |including |MAC.
P. |aeruginosa |Key |ID |Tests
Glucose |oxidizer
Oxidase |positive
Growth |at |42 |degrees
TSI: |A/NC
Produces |pyocyanin
Pseudomonas |aeruginosa
What |is |the |most |commonly |isolated |NFB?
Acinetobacter
What |is |the |2nd |most |commonly |isolated |NFB?
Which |species |of |Acinetobacter |are |saccharolytic |and |which |are |assacharolytic?
A. |baumannii |= |saccharolytic
A. |lwoffii |= |assaccharolytic
Acinetobacter |Key |ID |Tests
Oxidase |negative
Nitrate |negative
Glucose |utilization
Which |microbe |can |appear |as |pairs |of |plump |coccobacilli |or |as |filamentous |rods?
Acinetobacter
, What |is |the |3rd |most |commonly |isolated |NFB?
Stenotrophomonas |maltophilia
Key |ID |for |Stenotrophomonas |maltophilia
Oxidase |negative
Growth |on |MAC
Uses |glucose |and |maltose
Sensitive |to |SXT
Diseases |of |Burkholderia |cepacia
Important |nosocomial |pathogen
Cepacia |syndrome: |severe |resp |disease |in |CF |patients
What |color |is |B. |cepacia |on |BCSA |and |OFPBL |agar?
Yellow |- |produces |acid |from |lactose
What |microbe |has |a |strong, |earthy |odor?
Burkholderia |cepacia |(and |pseudomallei)
ID |for |B. |cepacia
Oxidase |positive |(weak)
Bipolar |staining |on |Gram |stain
What |microbe |causes |melioidosis, |an |aggressive |granulomatous |pulmonary |disease?
Burkholderia |pseudomallei
What |microbe |is |known |as |the |Vietnamese |time |bomb?
B. |pseudomallei
What |microbe |produces |wrinkled |colonies |on |lab |media?
B. |pseudomallei
ID |for |B. |pseudomallei
Oxidase |positive |(weak)
Moraxella |characteristics |on |Gram |stain
Pairs |of |plump |coccobacilli
Penicillin |test |is |used |to |differentiate |short |coccobacilli |to |true |cocci.
