STRAIGHTERLINE MICROBIOLOGY LAB
BIO250L LABS 1 2 3 4 5 6 7 8 FINAL PAPER
2026 COMPREHENSIVE QUESTIONS AND
ACCURATE SOLUTIONS GRADED A+
⩥ define the medium? Answer: liquid or gel designed to expose bacteria
⩥ what are the two types if medium and what do they provide? Answer:
broth and agar; they provide variable growth conditions in the form of
growth and stability
⩥ what three ways do you handle media? Answer: make sure that the top
is on the bottom; plate is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
⩥ define agar Answer: chemical derived from seaweed that solidifies
into a jelly-like substance and becomes a solid
⩥ what are two types of agar? Answer: plates and slants
⩥ example of agar? Answer: TSA--- trypticase soy agar
, ⩥ what is the air exposure procedure? Answer: open the plate to expose
it to the air for several minutes then seal with parafilm and place in an
incubate at 22C for 24 hours then 4C until the next week
⩥ what is the objective of a surface swab? Answer: to demonstrate that
their are microorganism and can only kill some
⩥ what is sterilization and some examples? Answer: when you kill all
the bacteria;
autoclave and radiation
⩥ what is autoclave? Answer: example of sterilization when you use
heat and pressure
⩥ what is disinfection and an example? Answer: only kills some or most
of the microorganisms; Lysol
⩥ when streaking plates, be sure to work close to the Bunsen burner
why?? Answer: because hot air rises creating a convection current
therefore all the bacteria floats up causing your work no to be
contaminated
⩥ cross hatch pattern is what and what is it used in? Answer: surface
swab and zigzag long was and up and down
BIO250L LABS 1 2 3 4 5 6 7 8 FINAL PAPER
2026 COMPREHENSIVE QUESTIONS AND
ACCURATE SOLUTIONS GRADED A+
⩥ define the medium? Answer: liquid or gel designed to expose bacteria
⩥ what are the two types if medium and what do they provide? Answer:
broth and agar; they provide variable growth conditions in the form of
growth and stability
⩥ what three ways do you handle media? Answer: make sure that the top
is on the bottom; plate is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
⩥ define agar Answer: chemical derived from seaweed that solidifies
into a jelly-like substance and becomes a solid
⩥ what are two types of agar? Answer: plates and slants
⩥ example of agar? Answer: TSA--- trypticase soy agar
, ⩥ what is the air exposure procedure? Answer: open the plate to expose
it to the air for several minutes then seal with parafilm and place in an
incubate at 22C for 24 hours then 4C until the next week
⩥ what is the objective of a surface swab? Answer: to demonstrate that
their are microorganism and can only kill some
⩥ what is sterilization and some examples? Answer: when you kill all
the bacteria;
autoclave and radiation
⩥ what is autoclave? Answer: example of sterilization when you use
heat and pressure
⩥ what is disinfection and an example? Answer: only kills some or most
of the microorganisms; Lysol
⩥ when streaking plates, be sure to work close to the Bunsen burner
why?? Answer: because hot air rises creating a convection current
therefore all the bacteria floats up causing your work no to be
contaminated
⩥ cross hatch pattern is what and what is it used in? Answer: surface
swab and zigzag long was and up and down
