CHEM 210 Biochemistry Exam | Portage
Learning | Questions & Verified Answers |
Latest 2025/2026 | 100% Verified | Graded
A+
T/F: According to the module, a compound with a molecular mass of 1,000 g/mol
is considered a macromolecule. - ANSWER false
macromolecule >5,000 g/mol
T/F: Biomolecules can have two or more different functional groups. -
ANSWER true
T/F: The following group is an amine.
C2H5NH2 - ANSWER true
T/F: In a eukaryotic cell, the organelles called peroxisomes serve the purpose of
digesting macromolecules. - ANSWER false
peroxisomes degrade dangerous/hazardous molecules, lysosomes degrade
macromolecules
,T/F: In a eukaryotic cell, the organelles called lysosomes serve the purpose of
digesting macromolecules. - ANSWER true
T/F: Carbon dioxide has a linear molecular shape AND has a bond angle of 109.5
degrees. - ANSWER false
linear shape = 180 degrees
Of the following, ___ is not a property of carbon.
Forming ionic bonds
Forming cyclic structures
Forming multiple bonds
Forming bonds with oxygen
Bonding in long chains - ANSWER Forming ionic bonds
What is responsible for the tertiary structure of proteins? -
ANSWER Interactions between amino acid side chains
Which of these agents can denature proteins?
A) Salt
B) Alcohol
C) UV light
D) All of the above - ANSWER D) All of the above
,Combining three amino acids produces what? - ANSWER A tripeptide + 2
water molecules
Disulfide bonds in proteins can be reduced to free sulfhydryl groups by reagents
such as _______. - ANSWER Beta-mercaptoethanol
What type of purification is based on the attraction of the protein for a particular
chemical group? - ANSWER Affinity chromatography
What affects the sedimentation of a particle? - ANSWER Mass
Shape
Density of the solution
Proteins can be separated from small molecules and ions through a semi-
permeable membrane by what process? - ANSWER Dialysis
In the Edman procedure for peptide sequence, what is used to selectively remove
the N-terminal residue as a PTH-derivative. - ANSWER Phenyl isothiocyanate
The first step in protein purification from a homogenate is usually what? -
ANSWER Isolation by centrifugation
Exclusion gel or gel-filtration chromatography separates molecules on what basis?
- ANSWER Size
, Polypeptides can be fragmented into smaller peptides by cleavage with
chymotrypsin, which hydrolyzes the peptide bond at the C-terminal side of what
amino acid residues? - ANSWER Phenylalanine
Tyrosine
Tryptophan
What gels are often used as the media for electrophoretic techniques such as SDS-
PAGE and isoelectric focusing? - ANSWER Polyacrylamide
The mobility of proteins in SDS-PAGE is inversely proportional to what? -
ANSWER The log of their masses
What is the advantage of adding SDS to gel electrophoresis? - ANSWER SDS
allows proteins to be separated on the basis of their approximate mass
What are some techniques that can be used to obtain information about protein
shape? - ANSWER X-ray crystallography
NMR spectroscopy
Which are some techniques that can be used for quantitative analysis? -
ANSWER ELISA
Absorbance spectroscopy
What is the information carrying intermediates of translation? -
ANSWER mRNA
Learning | Questions & Verified Answers |
Latest 2025/2026 | 100% Verified | Graded
A+
T/F: According to the module, a compound with a molecular mass of 1,000 g/mol
is considered a macromolecule. - ANSWER false
macromolecule >5,000 g/mol
T/F: Biomolecules can have two or more different functional groups. -
ANSWER true
T/F: The following group is an amine.
C2H5NH2 - ANSWER true
T/F: In a eukaryotic cell, the organelles called peroxisomes serve the purpose of
digesting macromolecules. - ANSWER false
peroxisomes degrade dangerous/hazardous molecules, lysosomes degrade
macromolecules
,T/F: In a eukaryotic cell, the organelles called lysosomes serve the purpose of
digesting macromolecules. - ANSWER true
T/F: Carbon dioxide has a linear molecular shape AND has a bond angle of 109.5
degrees. - ANSWER false
linear shape = 180 degrees
Of the following, ___ is not a property of carbon.
Forming ionic bonds
Forming cyclic structures
Forming multiple bonds
Forming bonds with oxygen
Bonding in long chains - ANSWER Forming ionic bonds
What is responsible for the tertiary structure of proteins? -
ANSWER Interactions between amino acid side chains
Which of these agents can denature proteins?
A) Salt
B) Alcohol
C) UV light
D) All of the above - ANSWER D) All of the above
,Combining three amino acids produces what? - ANSWER A tripeptide + 2
water molecules
Disulfide bonds in proteins can be reduced to free sulfhydryl groups by reagents
such as _______. - ANSWER Beta-mercaptoethanol
What type of purification is based on the attraction of the protein for a particular
chemical group? - ANSWER Affinity chromatography
What affects the sedimentation of a particle? - ANSWER Mass
Shape
Density of the solution
Proteins can be separated from small molecules and ions through a semi-
permeable membrane by what process? - ANSWER Dialysis
In the Edman procedure for peptide sequence, what is used to selectively remove
the N-terminal residue as a PTH-derivative. - ANSWER Phenyl isothiocyanate
The first step in protein purification from a homogenate is usually what? -
ANSWER Isolation by centrifugation
Exclusion gel or gel-filtration chromatography separates molecules on what basis?
- ANSWER Size
, Polypeptides can be fragmented into smaller peptides by cleavage with
chymotrypsin, which hydrolyzes the peptide bond at the C-terminal side of what
amino acid residues? - ANSWER Phenylalanine
Tyrosine
Tryptophan
What gels are often used as the media for electrophoretic techniques such as SDS-
PAGE and isoelectric focusing? - ANSWER Polyacrylamide
The mobility of proteins in SDS-PAGE is inversely proportional to what? -
ANSWER The log of their masses
What is the advantage of adding SDS to gel electrophoresis? - ANSWER SDS
allows proteins to be separated on the basis of their approximate mass
What are some techniques that can be used to obtain information about protein
shape? - ANSWER X-ray crystallography
NMR spectroscopy
Which are some techniques that can be used for quantitative analysis? -
ANSWER ELISA
Absorbance spectroscopy
What is the information carrying intermediates of translation? -
ANSWER mRNA
