EXAM SCRIPT 2026 QUESTIONS AND
SOLUTIONS GRADED A+
◉Acridine orange stain. Answer: -Acridine orange stains nucleic acid
of living and dead cells
-Bacteria and yeast
◉What plates do we subculture CSF?
What temperature and atmosphere is needed?. Answer: -BAP, CHOC,
Thio
-37°C in CO2
◉What are common CSF pathogens?. Answer: -H. influenzae
-S. pneumoniae
-N. meningitidis
-GBS
-Listeria
-CNS
,◉What is CSF rhinorrhea?. Answer: CSF leaking through nasal
passage due to blunt trauma, skull fracture or a tumor
◉What plates do we subculture abscess's and wound cultures?
What temperature and atmosphere is needed?. Answer: -BAP, CHOC,
MAC/EMB, CNA/PEA
-anaerobic if requested
-37°C in CO2
***Pus is the best specimen***
◉What are common abscess and wound culture pathogens?.
Answer: -Staph Aureus
-GAS
-Enterics
-Anaerobes
-PSAR
-Enterococci
◉What plates do we subculture stool?
What temperature and atmosphere is needed?. Answer: -BAP,
MAC/EMB, HE/SS/XLD, GN broth
-37°C in air
, ◉Special stool culture plates. Answer: CampyBAP, CIN, TCBS, SMAC
◉What plates do we subculture genital specimens?
What temperature and atmosphere is needed?. Answer: -BAP, MTM
-37°C in CO2
-Gram stain genital specimens and look for the following; clue cells,
gram negative diplococci, yeast
◉What plates do we subculture sinus specimens?
What temperature and atmosphere is needed?. Answer: -BAP, CHOC,
broth, anaerobic media
-37°C in CO2
◉What are common sinus pathogens?. Answer: -S. pneumoniae
-M. catarrhalis
-H. influenzae
◉What plates do we subculture otitis media specimens?
What temperature and atmosphere is needed?. Answer: -BAP, CHOC,
broth, anaerobic media
-35°C in CO2