PAPER 2026 COMPLETE QUESTIONS AND
ANSWERS GRADED A+
◉Conventional culture methods for mycobacteria. Answer:
Temperature - 35-37°C (most mycobacteria prefer this temperature,
a few do not)
Atmosphere- 5-10% CO2
Incubate in the dark
Incubate for 8 weeks
◉Non-selective egg-based culture media. Answer: -Lowenstein-
Jensen (LJ)
-Petragnani
◉Selective egg-based media. Answer: -LJ-graft
-Mycobactosel-LJ
◉Nonselective agar-based culture media. Answer: -Middlebrook
7H10 & 7H11
, ◉Selective agar-based culture media. Answer: -Mitchiison's selective
7H11
-Mycobactosel-middlebrook 7H10
◉Mycobacteria absecssus. Answer: -Soil and water
-Wounds
-Pulmonary disease from CF patient
-Rapid grower
◉Photochromogens. Answer: -Only produce pigment when exposed
to light
-M. kansaii
-M. marinum
◉Scotochromgens. Answer: Produces pigment in both light and dark
-M. scrofulaceum
-M. gordonae
-M. xenopi
◉Nonphotochromogens. Answer: No pigment in light or dark