1|Page
MICROBIOLOGY LAB FINAL EXAM VERSION 3 EXAM LATEST 2026-
2027 ACTUAL EXAM WITH COMPLETE QUESTIONS AND CORRECT
DETAILED ANSWERS (100% VERIFIED ANSWERS) |ALREADY
GRADED A+| ||PROFESSOR VERIFIED|| ||BRANDNEW!!!||
In the bacterial transformation exercise, the term transformation
refers to... - ANSWER-The ability of a bacterial cell to pick up
DNA from the environment and to add this new DNA to its
genome
Match the following steps of PCR with the process that occurs
during that step. - ANSWER-Denaturation: DNA unwinds and the
two strands of DNA separate
Annealing: Primers attach to the template DNA
Extension: DNA polymerase adds nucleotides to make a
complimentary strand of DNA
Among the "Top 5" bacteria we use, ______________
______________ has a positive Gram reaction and has coccus-
shaped cells. - ANSWER-Staphylococcus epidermidis
,2|Page
Brightfield microscopy is used to view live, unstained organisms. -
ANSWER-False
When phenol red turns yellow, this indicates a negative
carbohydrate fermentation test result. - ANSWER-False
Match each term to the best definition. - ANSWER-Conjugation:
The transfer of DNA between two bacterial cells that requires cell-
to-cell contact
Transduction: The transfer of DNA between bacterial cells that
requires a bacteriophage
Transfection: The uptake of bacteriophage DNA from the
environment
Transformation: The uptake of naked DNA from the environment
What are the main factors to obtain a good image used in
microscopy? Select all correct answers. - ANSWER-Resolution
Magnification
Contrast
, 3|Page
Indicate if the following actions are examples of aseptic technique
or if they are not examples of aseptic technique. - ANSWER-
Changing the pipette tip if it touches the benchtop: This is an
example of aseptic technique
Opening serological pipettes at the tip side: This is not an
example of aseptic technique
Passing open test tubes through the flame: This is an example of
aseptic technique
Placing caps and lids on the benchtop: This is not an example of
aseptic technique
Transferring different liquids with the same pipette tip: This is not
an example of aseptic technique
Working with multiple test tubes to limit the amount of time each
tube is opened and exposed: This is an example of aseptic
technique
A bacterial species that is not naturally competent cannot have its
genome altered. - ANSWER-False
MICROBIOLOGY LAB FINAL EXAM VERSION 3 EXAM LATEST 2026-
2027 ACTUAL EXAM WITH COMPLETE QUESTIONS AND CORRECT
DETAILED ANSWERS (100% VERIFIED ANSWERS) |ALREADY
GRADED A+| ||PROFESSOR VERIFIED|| ||BRANDNEW!!!||
In the bacterial transformation exercise, the term transformation
refers to... - ANSWER-The ability of a bacterial cell to pick up
DNA from the environment and to add this new DNA to its
genome
Match the following steps of PCR with the process that occurs
during that step. - ANSWER-Denaturation: DNA unwinds and the
two strands of DNA separate
Annealing: Primers attach to the template DNA
Extension: DNA polymerase adds nucleotides to make a
complimentary strand of DNA
Among the "Top 5" bacteria we use, ______________
______________ has a positive Gram reaction and has coccus-
shaped cells. - ANSWER-Staphylococcus epidermidis
,2|Page
Brightfield microscopy is used to view live, unstained organisms. -
ANSWER-False
When phenol red turns yellow, this indicates a negative
carbohydrate fermentation test result. - ANSWER-False
Match each term to the best definition. - ANSWER-Conjugation:
The transfer of DNA between two bacterial cells that requires cell-
to-cell contact
Transduction: The transfer of DNA between bacterial cells that
requires a bacteriophage
Transfection: The uptake of bacteriophage DNA from the
environment
Transformation: The uptake of naked DNA from the environment
What are the main factors to obtain a good image used in
microscopy? Select all correct answers. - ANSWER-Resolution
Magnification
Contrast
, 3|Page
Indicate if the following actions are examples of aseptic technique
or if they are not examples of aseptic technique. - ANSWER-
Changing the pipette tip if it touches the benchtop: This is an
example of aseptic technique
Opening serological pipettes at the tip side: This is not an
example of aseptic technique
Passing open test tubes through the flame: This is an example of
aseptic technique
Placing caps and lids on the benchtop: This is not an example of
aseptic technique
Transferring different liquids with the same pipette tip: This is not
an example of aseptic technique
Working with multiple test tubes to limit the amount of time each
tube is opened and exposed: This is an example of aseptic
technique
A bacterial species that is not naturally competent cannot have its
genome altered. - ANSWER-False
