Serology Test V EXAM STUDY
GUIDE 2026/2027 COMPLETE
QUESTIONS WITH VERIFIED
CORRECT ANSWERS || 100%
GUARANTEED PASS NEWEST
VERSION
Description:
Prepare thoroughly for your Clinical Immunology and Serology exams with this comprehensive
study guide for the years 2026 and 2027. This guide includes a detailed collection of the most
relevant and up-to-date questions, each accompanied by verified correct answers to ensure
you’re fully prepared for your exams. Topics covered range from antigen-antibody reactions to
key immunology techniques such as agglutination, precipitation, and immunodiffusion. You'll
find a wealth of essential terms, laboratory procedures, and diagnostic serology concepts that are
critical to mastering this field. This study guide also includes flashcards for quick revision,
making it easy to test your knowledge on the go. Designed to help you achieve a 100%
guaranteed pass, this newest version is the most complete and reliable resource for mastering
Clinical Immunology and Serology, giving you the confidence needed for success on exam day.
Keywords:
EXAM STUDY GUIDE 2026/2027, CLINICAL
IMMUNOLOGY EXAM, SEROLOGY EXAM
STUDY, IMMUNOLOGY QUESTIONS,
SEROLOGY QUESTIONS, GUARANTEED
PASS, VERIFIED ANSWERS, ANTIGEN
ANTIBODY REACTIONS, AGGLUTINATION,
PRECIPITATION, IMMUNODIFFUSION,
HYPERSENSITIVITY, LABORATORY
PROCEDURES, AUTOIMMUNITY,
, DIAGNOSTIC SEROLOGY, MEDICAL EXAM
PREPARATION
Which disease is caused by Borrelia burgdorferi - spirochete, transmitted through
bites of Ixodes tick carried by white-tailed deer with stages of disease being (1)
Localized rash, (2) dissemination to multiple organs such as skin, nervous system, heart
and joints and (3) chronic disseminated stage with symptoms of arthritis? -
ANSWER✅✅✅Lyme Disease
Which disease involves IgM response occurs 3 - 6 weeks after bite and the presence of
B. burgdorferi confirmed by direct (culture) or indirect methods (serology)? -
ANSWER✅✅✅Lyme disease
What are the laboratory testing involved in diagnosing lyme disease? -
ANSWER✅✅✅Indirect immunofluorescence, Enzyme immunoassay, Immunoblotting
(western blot) and PCR.
Which technique is Used to screen with the antigen on slide (spirochete), titers of 256 is
considered positive, false negatives may occur in specimens collected early in infection,
false positives are caused by syphilis, rheumatoid arthritis, etc. and positives are
confirmed by Western Blot? -ANSWER✅✅✅IFA for lyme's disease
Which techniques is Used to screen, it is more precise than IFA, it lacks sensitivity in
early stages of disease causing false negatives, false positives due to syphilis, relapsing
fever, leptospirosis, infectious mononucleosis, Rocky Mountain spotted fever and can
run RPR to rule out syphilis? -ANSWER✅✅✅ELISA for Lyme's disease
Which technique is recommended confirmatory test by Electrophoresis of B. burgdorferi
antigens in acrylamide gel, transfer of antigens to nitrocellulose paper, paper is reacted
with patient serum, antihuman globulin with enzyme tag added, washed and enzyme
substrate added then protein bands are numbered according to molecular weight? -
ANSWER✅✅✅Western Blot for Lyme's disease
What is the interpretation of western blot for lyme's disease? -ANSWER✅✅✅IgM
antibody is positive if 2 of the following bands are positive at 23, 39, 42 kD. IgG antibody
is positive if 5 of the following bands are positive
18, 23, 28, 30, 39, 41, 45, 58, 66, 93 kD.
Which technique has the best sensitivity and specificity, DNA from patient (skin from
rash area) is extracted and amplified
,DNA probe (target DNA found only in strains of B. burgdorferi) is added, if positive, the
single stranded DNA probe will bind to the exact complimentary strand of patient DNA,
no cross reactivity with other spirochete antigens? -ANSWER✅✅✅PCR for Lyme's
disease
What is the causative agent for toxplasmosis? -ANSWER✅✅✅Toxoplasma gondii an
protozoan parasite.
What infectious disease is transmitted through ingestion of contaminated soil, cat litter
or infected raw or partially cooked pork, mutton, or beef, blood transfusion, organ
transplant and congenitally? -ANSWER✅✅✅Toxoplasmosis.
What is possible of congenitally transmitted toxoplasmosis? -ANSWER✅✅✅Death
may occur if exposed in 1st trimester. Later infection may cause blindness,
hydrocephaly, or other CNS damage. It isn't transmitted if mother is exposed prior to
pregnancy.
Which infectious diseases consists of nearly 40% of the world's population being
infected, usually asymptomatic or mild lymphadenopathy and can be sever in
immunocompromised? -ANSWER✅✅✅Toxoplasmosis.
What is the method of infection of toxoplasmosis and how does it affect phagocytosis? -
ANSWER✅✅✅The organism replicates in macrophages and can survive indefinitely
due to its ability to prevent fusion of lysosomes with phagosomes and stop
phagocytosis.
What are the serological testing for toxoplasmosis? -ANSWER✅✅✅Isolation of the
organism, EIA for IgM or IgG, IFA for IgG, specific IgM (detectable) and IgA testing of
newborns and PCR for prenatal testing on amniotic fluid.
What is the method of choice when performing serological testing for toxoplasmosis? -
ANSWER✅✅✅EIA for IgM or IgG.
Which infectious disease causes infectious gastroenteritis, occurs in infants and small
children usually in winter or spring and is transmitted through oral-fecal route? -
ANSWER✅✅✅Rotavirus.
What are the serological testing done for rotavirus? -ANSWER✅✅✅Direct EIA and
Indirect EIA.
Which technique test for the viral antigen of rotavirus? -ANSWER✅✅✅Direct EIA.
Which technique test for the antibody to the viral antigen of rotavirus? -
ANSWER✅✅✅Indirect EIA
, Which infections disease is caused by a ssRNA virus, has respiratory route of entry with
symptoms including bilateral or unilateral parotitis (infection of salivary glands: 30-40%
of cases) and can spread to meninges of brain, salivary glands, pancreas, testes, and
ovaries causing inflammation? -ANSWER✅✅✅Mumps.
Which infectious disease has an incubation period of 12 to 25 days and have a live
attenuated vaccine available? -ANSWER✅✅✅Mumps.
What is the issue with isolating Toxoplama gondii? -ANSWER✅✅✅It is difficult and
impractical.
What are the laboratory testing for mumps that are important in diagnosing cases
without parotitis? -ANSWER✅✅✅Viral culture, serological testing and RT-PCR.
What is the issue with performing a viral culture in laboratory testing for mumps? -
ANSWER✅✅✅It is slow and difficult.
Serological testing including ELISA and IFA are considered the method of choice. What
are some other serological test that can be performed for mumps? -
ANSWER✅✅✅complement fixation, hemagglutination inhibition, neutralization assays.
Which commonly used serological test involve solid-phase mumps specific IgM capture
assay reducing false positives dure to RF and specific mumps IgM requiring serial
testing for diagnosis of new case? -ANSWER✅✅✅ELISA and IFA.
Which technique is not widely used at this time for mumps but is available? -
ANSWER✅✅✅RT-PCR.
Which infectious disease is caused by the rubeola virus (ssRNA) with an incubation
period of 10 to 12 days having symptoms to include fever, cough, runny nose,
conjunctivitis and eythematous macropapular rash appearing 14 days after exposure
and have a vaccine that is usually administered in combo with MMR? -
ANSWER✅✅✅Measles (Rubeola).
What are the laboratory testing used for measles? -ANSWER✅✅✅Viral culture and
serological testing.
What serological testing are involve in testing for measles? -ANSWER✅✅✅ELISA,
Hemagglutination inhibition, neutralization, complement fixation, IFA and RT-PCR.
Which serological testing consist of Rubeola specific IgM (IgM capture assay reducing
false positive due to rheumatoid factor) and IgG and is most commonly used? -
ANSWER✅✅✅ELISA.
GUIDE 2026/2027 COMPLETE
QUESTIONS WITH VERIFIED
CORRECT ANSWERS || 100%
GUARANTEED PASS NEWEST
VERSION
Description:
Prepare thoroughly for your Clinical Immunology and Serology exams with this comprehensive
study guide for the years 2026 and 2027. This guide includes a detailed collection of the most
relevant and up-to-date questions, each accompanied by verified correct answers to ensure
you’re fully prepared for your exams. Topics covered range from antigen-antibody reactions to
key immunology techniques such as agglutination, precipitation, and immunodiffusion. You'll
find a wealth of essential terms, laboratory procedures, and diagnostic serology concepts that are
critical to mastering this field. This study guide also includes flashcards for quick revision,
making it easy to test your knowledge on the go. Designed to help you achieve a 100%
guaranteed pass, this newest version is the most complete and reliable resource for mastering
Clinical Immunology and Serology, giving you the confidence needed for success on exam day.
Keywords:
EXAM STUDY GUIDE 2026/2027, CLINICAL
IMMUNOLOGY EXAM, SEROLOGY EXAM
STUDY, IMMUNOLOGY QUESTIONS,
SEROLOGY QUESTIONS, GUARANTEED
PASS, VERIFIED ANSWERS, ANTIGEN
ANTIBODY REACTIONS, AGGLUTINATION,
PRECIPITATION, IMMUNODIFFUSION,
HYPERSENSITIVITY, LABORATORY
PROCEDURES, AUTOIMMUNITY,
, DIAGNOSTIC SEROLOGY, MEDICAL EXAM
PREPARATION
Which disease is caused by Borrelia burgdorferi - spirochete, transmitted through
bites of Ixodes tick carried by white-tailed deer with stages of disease being (1)
Localized rash, (2) dissemination to multiple organs such as skin, nervous system, heart
and joints and (3) chronic disseminated stage with symptoms of arthritis? -
ANSWER✅✅✅Lyme Disease
Which disease involves IgM response occurs 3 - 6 weeks after bite and the presence of
B. burgdorferi confirmed by direct (culture) or indirect methods (serology)? -
ANSWER✅✅✅Lyme disease
What are the laboratory testing involved in diagnosing lyme disease? -
ANSWER✅✅✅Indirect immunofluorescence, Enzyme immunoassay, Immunoblotting
(western blot) and PCR.
Which technique is Used to screen with the antigen on slide (spirochete), titers of 256 is
considered positive, false negatives may occur in specimens collected early in infection,
false positives are caused by syphilis, rheumatoid arthritis, etc. and positives are
confirmed by Western Blot? -ANSWER✅✅✅IFA for lyme's disease
Which techniques is Used to screen, it is more precise than IFA, it lacks sensitivity in
early stages of disease causing false negatives, false positives due to syphilis, relapsing
fever, leptospirosis, infectious mononucleosis, Rocky Mountain spotted fever and can
run RPR to rule out syphilis? -ANSWER✅✅✅ELISA for Lyme's disease
Which technique is recommended confirmatory test by Electrophoresis of B. burgdorferi
antigens in acrylamide gel, transfer of antigens to nitrocellulose paper, paper is reacted
with patient serum, antihuman globulin with enzyme tag added, washed and enzyme
substrate added then protein bands are numbered according to molecular weight? -
ANSWER✅✅✅Western Blot for Lyme's disease
What is the interpretation of western blot for lyme's disease? -ANSWER✅✅✅IgM
antibody is positive if 2 of the following bands are positive at 23, 39, 42 kD. IgG antibody
is positive if 5 of the following bands are positive
18, 23, 28, 30, 39, 41, 45, 58, 66, 93 kD.
Which technique has the best sensitivity and specificity, DNA from patient (skin from
rash area) is extracted and amplified
,DNA probe (target DNA found only in strains of B. burgdorferi) is added, if positive, the
single stranded DNA probe will bind to the exact complimentary strand of patient DNA,
no cross reactivity with other spirochete antigens? -ANSWER✅✅✅PCR for Lyme's
disease
What is the causative agent for toxplasmosis? -ANSWER✅✅✅Toxoplasma gondii an
protozoan parasite.
What infectious disease is transmitted through ingestion of contaminated soil, cat litter
or infected raw or partially cooked pork, mutton, or beef, blood transfusion, organ
transplant and congenitally? -ANSWER✅✅✅Toxoplasmosis.
What is possible of congenitally transmitted toxoplasmosis? -ANSWER✅✅✅Death
may occur if exposed in 1st trimester. Later infection may cause blindness,
hydrocephaly, or other CNS damage. It isn't transmitted if mother is exposed prior to
pregnancy.
Which infectious diseases consists of nearly 40% of the world's population being
infected, usually asymptomatic or mild lymphadenopathy and can be sever in
immunocompromised? -ANSWER✅✅✅Toxoplasmosis.
What is the method of infection of toxoplasmosis and how does it affect phagocytosis? -
ANSWER✅✅✅The organism replicates in macrophages and can survive indefinitely
due to its ability to prevent fusion of lysosomes with phagosomes and stop
phagocytosis.
What are the serological testing for toxoplasmosis? -ANSWER✅✅✅Isolation of the
organism, EIA for IgM or IgG, IFA for IgG, specific IgM (detectable) and IgA testing of
newborns and PCR for prenatal testing on amniotic fluid.
What is the method of choice when performing serological testing for toxoplasmosis? -
ANSWER✅✅✅EIA for IgM or IgG.
Which infectious disease causes infectious gastroenteritis, occurs in infants and small
children usually in winter or spring and is transmitted through oral-fecal route? -
ANSWER✅✅✅Rotavirus.
What are the serological testing done for rotavirus? -ANSWER✅✅✅Direct EIA and
Indirect EIA.
Which technique test for the viral antigen of rotavirus? -ANSWER✅✅✅Direct EIA.
Which technique test for the antibody to the viral antigen of rotavirus? -
ANSWER✅✅✅Indirect EIA
, Which infections disease is caused by a ssRNA virus, has respiratory route of entry with
symptoms including bilateral or unilateral parotitis (infection of salivary glands: 30-40%
of cases) and can spread to meninges of brain, salivary glands, pancreas, testes, and
ovaries causing inflammation? -ANSWER✅✅✅Mumps.
Which infectious disease has an incubation period of 12 to 25 days and have a live
attenuated vaccine available? -ANSWER✅✅✅Mumps.
What is the issue with isolating Toxoplama gondii? -ANSWER✅✅✅It is difficult and
impractical.
What are the laboratory testing for mumps that are important in diagnosing cases
without parotitis? -ANSWER✅✅✅Viral culture, serological testing and RT-PCR.
What is the issue with performing a viral culture in laboratory testing for mumps? -
ANSWER✅✅✅It is slow and difficult.
Serological testing including ELISA and IFA are considered the method of choice. What
are some other serological test that can be performed for mumps? -
ANSWER✅✅✅complement fixation, hemagglutination inhibition, neutralization assays.
Which commonly used serological test involve solid-phase mumps specific IgM capture
assay reducing false positives dure to RF and specific mumps IgM requiring serial
testing for diagnosis of new case? -ANSWER✅✅✅ELISA and IFA.
Which technique is not widely used at this time for mumps but is available? -
ANSWER✅✅✅RT-PCR.
Which infectious disease is caused by the rubeola virus (ssRNA) with an incubation
period of 10 to 12 days having symptoms to include fever, cough, runny nose,
conjunctivitis and eythematous macropapular rash appearing 14 days after exposure
and have a vaccine that is usually administered in combo with MMR? -
ANSWER✅✅✅Measles (Rubeola).
What are the laboratory testing used for measles? -ANSWER✅✅✅Viral culture and
serological testing.
What serological testing are involve in testing for measles? -ANSWER✅✅✅ELISA,
Hemagglutination inhibition, neutralization, complement fixation, IFA and RT-PCR.
Which serological testing consist of Rubeola specific IgM (IgM capture assay reducing
false positive due to rheumatoid factor) and IgG and is most commonly used? -
ANSWER✅✅✅ELISA.
