Forensic Biology Exam 3 UPDATED
ACTUAL QUESTIONS AND CORRECT
ANSWERS
What is an alternate light source and what it is used for? - CORRECT ANSWER A special Blue
light and orange filters
-It is a screening test for the identification of body fluid
When an alternate light source is used in a piece of evidence, only the semen will fluoresce.
True/False - CORRECT ANSWER False- saliva, semen, and urine
Large piece of evidence such as bed sheets are examined first by acid phosphatase test before other
methods are employed. True/False - CORRECT ANSWER False, first examined by an alternate
light source to see if any areas fluoresce, then AP mapping
The process of screening small pieces of evidence such as panties for the presence of seminal fluid is
commonly referred to as __________. - CORRECT ANSWER AP Mapping or press test
Who was the Dutch scientist that identified spermatozoa/morphology of spermatozoa under the
microscope? When? - CORRECT ANSWER Leeuwenhoek, 1677
The Dutch scientist Leeuwenhoek presented his findings regarding spermatozoa to this society -
CORRECT ANSWER Philosophical Transactions of the Royal Society of London
What are the five things the Dutch scientist explained in his speech to the Philosophical Transactions
of the Royal Society of London regarding his discovery of spermatozoa (characteristics and
description). - CORRECT ANSWER 1. Number - Several million
2. Shape- Bodies were round, blunt in the front, and ran to a point behind head and tail
3. Suspension - In a thicker substance
4. speed - They had to lash their tails 7-8 times to move the width of a hair
5. size- Smaller than a corpuscle
, List any ten items of evidence that are collected during a sexual assault examination. - CORRECT
ANSWER - Outer clothing worn at the time of the assault
- Underwear worn at the time of the assault
- Clothing worn at the time of the exam
- Fingernail scrapings
- Rectal swab
- Vagina/penile swab
- Oral swab
- Buccal swab (cheek)
- Head hair from victim for reference
- Pubic hair from victim for reference
- Two vials of blood from victim for reference
- Urine sample from victim
The three presumptive tests that can be used to detect the presence of seminal fluid. - CORRECT
ANSWER 1. Acid Phosphatase
2. Barberios Crystal Test
3. Florence Crystal Test
The enzyme acid phosphatase is found in approximately ____________times more in semen
compared to other body fluids. - CORRECT ANSWER 400
Acid phosphatase is an enzyme that is active at a pH above 7. True/False - CORRECT
ANSWER False, acid
Acid Phosphatase can also be called as - CORRECT ANSWER Prostate Phosphate
Acid Phosphatase is highly stable at 37°C. True/False - CORRECT ANSWER False
The average acid phosphatase concentration found in human seminal fluid is _________ king-
armstrong units/100ml semen - CORRECT ANSWER 370,000
ACTUAL QUESTIONS AND CORRECT
ANSWERS
What is an alternate light source and what it is used for? - CORRECT ANSWER A special Blue
light and orange filters
-It is a screening test for the identification of body fluid
When an alternate light source is used in a piece of evidence, only the semen will fluoresce.
True/False - CORRECT ANSWER False- saliva, semen, and urine
Large piece of evidence such as bed sheets are examined first by acid phosphatase test before other
methods are employed. True/False - CORRECT ANSWER False, first examined by an alternate
light source to see if any areas fluoresce, then AP mapping
The process of screening small pieces of evidence such as panties for the presence of seminal fluid is
commonly referred to as __________. - CORRECT ANSWER AP Mapping or press test
Who was the Dutch scientist that identified spermatozoa/morphology of spermatozoa under the
microscope? When? - CORRECT ANSWER Leeuwenhoek, 1677
The Dutch scientist Leeuwenhoek presented his findings regarding spermatozoa to this society -
CORRECT ANSWER Philosophical Transactions of the Royal Society of London
What are the five things the Dutch scientist explained in his speech to the Philosophical Transactions
of the Royal Society of London regarding his discovery of spermatozoa (characteristics and
description). - CORRECT ANSWER 1. Number - Several million
2. Shape- Bodies were round, blunt in the front, and ran to a point behind head and tail
3. Suspension - In a thicker substance
4. speed - They had to lash their tails 7-8 times to move the width of a hair
5. size- Smaller than a corpuscle
, List any ten items of evidence that are collected during a sexual assault examination. - CORRECT
ANSWER - Outer clothing worn at the time of the assault
- Underwear worn at the time of the assault
- Clothing worn at the time of the exam
- Fingernail scrapings
- Rectal swab
- Vagina/penile swab
- Oral swab
- Buccal swab (cheek)
- Head hair from victim for reference
- Pubic hair from victim for reference
- Two vials of blood from victim for reference
- Urine sample from victim
The three presumptive tests that can be used to detect the presence of seminal fluid. - CORRECT
ANSWER 1. Acid Phosphatase
2. Barberios Crystal Test
3. Florence Crystal Test
The enzyme acid phosphatase is found in approximately ____________times more in semen
compared to other body fluids. - CORRECT ANSWER 400
Acid phosphatase is an enzyme that is active at a pH above 7. True/False - CORRECT
ANSWER False, acid
Acid Phosphatase can also be called as - CORRECT ANSWER Prostate Phosphate
Acid Phosphatase is highly stable at 37°C. True/False - CORRECT ANSWER False
The average acid phosphatase concentration found in human seminal fluid is _________ king-
armstrong units/100ml semen - CORRECT ANSWER 370,000
