UNE BIOL 1020 - Unit 2 Exam
Questions and Correct Answers.
What structure is indicated by: 10A, 15T, 3G, 7C? - Answer Single-stranded DNA.
Negative control means that a regulator molecule is - Answer bound and transcription is
inhibited AND removed and transcription begins.
DNA is characterized by which of the following feature(s)? - Answer Deoxyribose AND
Thymine.
All fungi have __________ in their cell walls - Answer chitin.
The placement of the amino acid during translation is determined by the - Answer
complementarity of the codon-anticodon.
Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case,
are 2 primers (a forward and a reverse) necessary? - Answer No; dideoxynucleotide
sequencing depends on different length fragments being formed and then separated based on
size and this can take place with only a specific forward primer OR a specific reverse primer.
Treponema and Borrelia - Answer are spirochetes.
Coliforms - Answer A. are an informal grouping of enterics.
B. ferment lactose.
C. includes E. coli.
D. are used as indicators of fecal contamination.
->E. All of the choices are correct.
A common way to identify E. coli that carry the desired recombinant DNA is by using a - Answer
probe
Thymine dimer-containing section of DNA is cut out, resynthesized and sealed - Answer
Excision repair
, Catastrophic DNA damage caused by ultraviolet light can be survived by the cell with this -
Answer SOS repair
Non-constituitive system with visible light energy breaking covalent T-T bonds - Answer
Photoreactivation
Multiple enzymes may repair an A-C error left unrecognized by DNA polymerase - Answer
Mismatch repair
A "spell check" function by the normal replication enzyme to ensure fidelity - Answer
Proofreading
Outside of living cells, viruses are - Answer metabolically inert.
The size of the amplified DNA fragment generated during PCR is determined by - Answer the
locations to which the primers anneal.
Direct selection involves inoculating cells onto growth media upon which - Answer the
mutant, but not the parental cell type, will grow.
When DNA probes are used to identify bacterial DNA similarities by hybridization, the probe
DNA is heated and the template DNA is treated to separate the 2 strands. Why would the probe
DNA be heated? - Answer The probe DNA is single stranded but it may have folded back on
itself and formed portions that are double-stranded; heating it up breaks any possible hydrogen
bonds that may have formed, returning it to a single-stranded state prior to hybridization.
Strain differences are helpful in - Answer tracing the source of outbreaks of disease.
Bacteria use ___________ attached to the polymerase and they direct RNA polymerase to
promoters; eukaryotic cells use __________ that instead attach directly to the DNA first. -
Answer sigma factors; transcription factors
Disagreements between conclusions obtained from rDNA data and other techniques may be
explained by - Answer horizontal DNA transfer.
The Ames test is useful as a rapid screening test to identify those compounds that - Answer
have a high probability of being carcinogenic.
To increase the proportion of mutants in a population of bacteria one may use - Answer
penicillin enrichment.
Questions and Correct Answers.
What structure is indicated by: 10A, 15T, 3G, 7C? - Answer Single-stranded DNA.
Negative control means that a regulator molecule is - Answer bound and transcription is
inhibited AND removed and transcription begins.
DNA is characterized by which of the following feature(s)? - Answer Deoxyribose AND
Thymine.
All fungi have __________ in their cell walls - Answer chitin.
The placement of the amino acid during translation is determined by the - Answer
complementarity of the codon-anticodon.
Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case,
are 2 primers (a forward and a reverse) necessary? - Answer No; dideoxynucleotide
sequencing depends on different length fragments being formed and then separated based on
size and this can take place with only a specific forward primer OR a specific reverse primer.
Treponema and Borrelia - Answer are spirochetes.
Coliforms - Answer A. are an informal grouping of enterics.
B. ferment lactose.
C. includes E. coli.
D. are used as indicators of fecal contamination.
->E. All of the choices are correct.
A common way to identify E. coli that carry the desired recombinant DNA is by using a - Answer
probe
Thymine dimer-containing section of DNA is cut out, resynthesized and sealed - Answer
Excision repair
, Catastrophic DNA damage caused by ultraviolet light can be survived by the cell with this -
Answer SOS repair
Non-constituitive system with visible light energy breaking covalent T-T bonds - Answer
Photoreactivation
Multiple enzymes may repair an A-C error left unrecognized by DNA polymerase - Answer
Mismatch repair
A "spell check" function by the normal replication enzyme to ensure fidelity - Answer
Proofreading
Outside of living cells, viruses are - Answer metabolically inert.
The size of the amplified DNA fragment generated during PCR is determined by - Answer the
locations to which the primers anneal.
Direct selection involves inoculating cells onto growth media upon which - Answer the
mutant, but not the parental cell type, will grow.
When DNA probes are used to identify bacterial DNA similarities by hybridization, the probe
DNA is heated and the template DNA is treated to separate the 2 strands. Why would the probe
DNA be heated? - Answer The probe DNA is single stranded but it may have folded back on
itself and formed portions that are double-stranded; heating it up breaks any possible hydrogen
bonds that may have formed, returning it to a single-stranded state prior to hybridization.
Strain differences are helpful in - Answer tracing the source of outbreaks of disease.
Bacteria use ___________ attached to the polymerase and they direct RNA polymerase to
promoters; eukaryotic cells use __________ that instead attach directly to the DNA first. -
Answer sigma factors; transcription factors
Disagreements between conclusions obtained from rDNA data and other techniques may be
explained by - Answer horizontal DNA transfer.
The Ames test is useful as a rapid screening test to identify those compounds that - Answer
have a high probability of being carcinogenic.
To increase the proportion of mutants in a population of bacteria one may use - Answer
penicillin enrichment.
