ORIGIN OF
REPLICATION
PRESENTATION BY SAMIHA
, ORIGIN OF REPLICATION
The visualization of replicating chromosome was
first accomplished by J. Cairns in 1963 using the
technique called autoradiography. • Autoradiography is
a method of detecting and
localizing radioactive isotopes in macromolecules
by exposure to photographic emulsion that is
sensitive to low energy radiation. • Autoradiography is
particularly useful in studying DNA meabolism because
DNA can be specifically
labeled by growin cells on [H3]thymidine, the
tritiated deoxyribonucleoside of thimidine. • Thymidine
is incorporated exclusively into DNA;
it is not present in any other major component of
the cell.
REPLICATION
PRESENTATION BY SAMIHA
, ORIGIN OF REPLICATION
The visualization of replicating chromosome was
first accomplished by J. Cairns in 1963 using the
technique called autoradiography. • Autoradiography is
a method of detecting and
localizing radioactive isotopes in macromolecules
by exposure to photographic emulsion that is
sensitive to low energy radiation. • Autoradiography is
particularly useful in studying DNA meabolism because
DNA can be specifically
labeled by growin cells on [H3]thymidine, the
tritiated deoxyribonucleoside of thimidine. • Thymidine
is incorporated exclusively into DNA;
it is not present in any other major component of
the cell.
