BIOL 3201 Midterm (Auburn) Exam With
Complete Solutions
Gloves to be disposed of where? - ANSWER biohazard can
Slides/swabs to be disposed of where? - ANSWER sharps container
Tubes to be disposed of where? - ANSWER discard cart
Plates to be disposed of where? - ANSWER biohazard can
BSL 1 - ANSWER microorganisms not known to cause disease in healthy adults
BSL 2 - ANSWER indigenous microorganisms that can lead to diseases of
varying severity in healthy adults
BSL 3 - ANSWER indigenous or exotic microorganisms that cause serious or
potentially lethal disease through respiratory transmission
BSL 4 - ANSWER microorganisms that are dangerous and exotic with high risk of
aerosol transmitted infections; rarely are there treatments or vaccines for these
microorganisms, and the diseases they cause are frequently fatal
Ubiquity - ANSWER a term meaning microorganisms are everywhere
What is used in order to prevent contamination from microorganisms? -
ANSWER aseptic technique
Disinfectants - ANSWER can destroy vegetative cells and viruses but may not
destroy endospores
What is used to clean work area before and after work? - ANSWER lysol
How long should you let the loop cool after flaming? - ANSWER 10-15 seconds
What side of the plate should you label - ANSWER bottom/agar side
Brightfield - ANSWER simplest form of microscopy where light is either passed
through, or reflected off, a specimen; requires the use of stains to visualize cells
Darkfield - ANSWER contrast is created by a bright specimen on a dark
background; ideal for external, not great for internal structures; stains not
required
, Fluorescent - ANSWER uses high intensity illumination to excite fluorescent
molecules (fluorophores) in the sample
Framework - ANSWER structure of the microscope and composed of the arm
and base
Stage - ANSWER horizontal area that supports the microscope slide
Oculars - ANSWER eyepieces that typically provide magnification of 10x
Stage adjustment knobs - ANSWER allow for the holding and moving of the slide
around the area that supports the slide
Condenser - ANSWER collects and directs the light from the light source to the
slide and is located under the are that supports the slide
Objectives - ANSWER attached to a rotating nosepiece; have powers of 10x, 40x,
100x
Which lens use immersion oil? - ANSWER 100x
Diaphragm - ANSWER controls the amount of light that reaches the slide
Coarse focus knob - ANSWER used first to bring objects on slide into focus
Fine focus knob - ANSWER after moving to 40x and 100x, this is used to bring
objects on slide into focus
Numerical aperture - ANSWER a mathematical expression that describes how
the condenser lens concentrates and focuses the light rays from the light source
Immersion oil - ANSWER used with the 100X objective and fills the gap between
the objective and slide to form a continuous lens path which increases image
resolution by reducing light refraction
Resolving power - ANSWER the ability of a lens system to show two closely
spaced objects as distinct and separate
Parfocal - ANSWER The ability of a microscope to remain relatively in focus
when changing from lower power objective to a higher power objective
Working distance - ANSWER the distance between the bottom of the objective
lens and the slide
Complete Solutions
Gloves to be disposed of where? - ANSWER biohazard can
Slides/swabs to be disposed of where? - ANSWER sharps container
Tubes to be disposed of where? - ANSWER discard cart
Plates to be disposed of where? - ANSWER biohazard can
BSL 1 - ANSWER microorganisms not known to cause disease in healthy adults
BSL 2 - ANSWER indigenous microorganisms that can lead to diseases of
varying severity in healthy adults
BSL 3 - ANSWER indigenous or exotic microorganisms that cause serious or
potentially lethal disease through respiratory transmission
BSL 4 - ANSWER microorganisms that are dangerous and exotic with high risk of
aerosol transmitted infections; rarely are there treatments or vaccines for these
microorganisms, and the diseases they cause are frequently fatal
Ubiquity - ANSWER a term meaning microorganisms are everywhere
What is used in order to prevent contamination from microorganisms? -
ANSWER aseptic technique
Disinfectants - ANSWER can destroy vegetative cells and viruses but may not
destroy endospores
What is used to clean work area before and after work? - ANSWER lysol
How long should you let the loop cool after flaming? - ANSWER 10-15 seconds
What side of the plate should you label - ANSWER bottom/agar side
Brightfield - ANSWER simplest form of microscopy where light is either passed
through, or reflected off, a specimen; requires the use of stains to visualize cells
Darkfield - ANSWER contrast is created by a bright specimen on a dark
background; ideal for external, not great for internal structures; stains not
required
, Fluorescent - ANSWER uses high intensity illumination to excite fluorescent
molecules (fluorophores) in the sample
Framework - ANSWER structure of the microscope and composed of the arm
and base
Stage - ANSWER horizontal area that supports the microscope slide
Oculars - ANSWER eyepieces that typically provide magnification of 10x
Stage adjustment knobs - ANSWER allow for the holding and moving of the slide
around the area that supports the slide
Condenser - ANSWER collects and directs the light from the light source to the
slide and is located under the are that supports the slide
Objectives - ANSWER attached to a rotating nosepiece; have powers of 10x, 40x,
100x
Which lens use immersion oil? - ANSWER 100x
Diaphragm - ANSWER controls the amount of light that reaches the slide
Coarse focus knob - ANSWER used first to bring objects on slide into focus
Fine focus knob - ANSWER after moving to 40x and 100x, this is used to bring
objects on slide into focus
Numerical aperture - ANSWER a mathematical expression that describes how
the condenser lens concentrates and focuses the light rays from the light source
Immersion oil - ANSWER used with the 100X objective and fills the gap between
the objective and slide to form a continuous lens path which increases image
resolution by reducing light refraction
Resolving power - ANSWER the ability of a lens system to show two closely
spaced objects as distinct and separate
Parfocal - ANSWER The ability of a microscope to remain relatively in focus
when changing from lower power objective to a higher power objective
Working distance - ANSWER the distance between the bottom of the objective
lens and the slide
