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Endoparasite
A parasite that lives within a host.
Called an infection
Ectoparasite
lives on the surface of the host
Called an infestation
Usually insects or arachnids
Can serve as vectors for endoparasites
Autoinfection
A reinfection in which the host is its own source of infection from a source already
present in the body
The host promotes the infection
Example: child's fingernails in a pinworm infection
Child scratches anal area and picks up eggs and swallows them to allow the adult
pinworm to keep infecting the host
Example: Rhabtidiform larvae become filariform larvae in the gut
Eggs in intestinal mucosa release rhabtidiform larvae that become filariform larvae and
grow into adults to keep the infection going (see life cycle)
Re-infection
patient infected a second time with the same microorganism that caused the original
illness.
Hyperinfection
Repeated reinfection with larvae from a parasite already in the host.
Generally results in a high parasitic load
Families of parasites
1. Protozoa = Amoebae, flagellates, ciliates, sporozoans, coccidia, and microsporidia
2. Nematodes = roundworms
3. Platyhelminthes = flatworms (cestodes, trematodes)
4. Pentastomids = tongue worms*
5. Acanthocephala = thorny headed worms*
6. Arthropods (ectoparasites)
*not discussed in this course
Parts of a life cycle
1. Definitive host (DH) = where the parasite reaches sexual maturity
2. Intermediate host (IH) = needed by the parasite to complete its life cycle
,3. Vector = host that transmits the parasite to the DH or IH; can also serve as a DH
or IH
4. Transport Host (TH) = Transmits the organism but does not play a role in the
reproduction of the organism
5. Reservoir (R) = source of the parasite; can be animate (like an animal or human) or
inaminate (like a body of water)
Laboratory Detection of parasites
Specimen source will be dependent on type of infection
Collect specimen in a media that promotes nutritional growth of organism
Handling: Concentrate specimen to increase detection probability
Employ method enhancements if necessary (parasite specific)
Protozoans in GI tract
Pathogens and non pathogens are both found in the stool of affected patients
Troph and Cyst forms can be identified
Cysts = infective stage
Can be:
1. Amoebae
2. Flagellates
3. Ciliates
4. Coccidia
5. Microsporidia
Protozoans in the blood and tissue
Pathogens transmitted by mosquitoes
Troph and Cyst forms can be identified
Cysts = infective stage
Can be:
1. Amoebae such as Plasmodium
2. Flagellates such as Trypanosoma
Mosquito also acts as IH for protozoans
Spends time in blood and liver cells (tissues)
Protozoans in other body sites
CNS/Eye - Amoebae
Vaginal/Prostate - Flagellates
Coccidia - Blood and Muscle
Nematodes of GI tract
Roundworms found in stool
Eggs and/or adult worms found in stool
Filarial nematodes of the blood
Filarial roundworms found in blood
Microfilariae are identified in the blood
Nematodes in tissues
Encapsulated roundworms found in tissues
Cysts are identified in the tissue
Cestodes in the GI tract
, Tapeworms found in GI Tract
Eggs, Proglottids, or Scolex are identified in stool of affected patients
Cestodes in the tissue
Encapsulated tapeworms found in tissues
Muscle or other tissue biopsies required for identification
Cysticerci = Taenia solium
Echinococcus = hydatid cyst
Trematodes in the GI tract
Eggs and/or mature Flukes found in the stool of affected patients
Trematodes in the Liver or Lung
Lung: Eggs and/or Flukes found in the sputum of affected patients
Liver: Eggs and/or Flukes found in the liver cells/gallbladder (liver biopsy, bile) of
affected patients
Trematodes in the Blood
Eggs of Flukes found in the blood or urine
Urine = Schistosoma haematobium
Approach to identifying parasites
Site of infection = specimen source
Size of suspected parasite
Mature or immature forms identified
Other laboratory methods
Blood parasites
1. Thick and Thin smears
2. Buffy coat smear
*Specific tests for malaria
*QBC test for malaria
*BinaxNOW malaria (Parasite Ag)
Stool parasites
Ova and Parasite Examination (O&P)
Which of the following parasites causes a fatal CNS infection transmitted by the tsetse
fly in Africa? -ANSWER✅✅✅Trypanosoma brucei
The difference between sporogony (sexual phase) and schizogony (asexual phase) in
malaria is? -ANSWER✅✅✅Schizogony occurs in the erythrocytic cycle in humans
The paroxysm is important to clinically diagnose malaria and the lab diagnosis is
dependent upon seeing the growth stages of the Plasmodium. Both the paroxysm and
growth stages occur during? -ANSWER✅✅✅erythrocytic cycle in humans
Which of the following is NOT a characteristic of Schistosoma mansoni?
A) blood fluke
B) intermediate host is a snail
C) larva is the diagnostic stage
D) penetrates skin in water -ANSWER✅✅✅larva is the diagnostic stage