CHM2046L FINAL EXAM LATEST
UF SPRING 2026 - 2027
beer-lambert law - ANSWERS-A = εdc
A = absorbance
ε = molar absorptivity (L mol-1 cm-1)
d = distance the light travels through the solution (the cuvette) in cm
c = concentration of the solution (mol/L)
Beer's Law calibration curve:
Use the following data to generate a calibration curve of Absorbance vs
Concentration at λmax = 420.6 nm.
concentration 1: 6e-5 M; absorbance 1: 1.330
concentration 2: 2e-5 M; absorbance 1: 0.451
concentration 1: 1e-5 M; absorbance 1: 0.239
END OF
PAGE
1
, CHM2046L FINAL EXAM LATEST
UF SPRING 2026 - 2027
concentration 1: 2e-6 M; absorbance 1: 0.042
Record the equation and analyze your data:
- do all of the data points fall on the trendline? Is your R2 value greater
than 0.990?
- what does y represent?
- what does x represent?
- why should the y-intercept of the linear trendline be very close to 0? -
ANSWERS-equation: y=22066x + 0.008
yes, all points fall on the trendline or are very close. R2 = 0.9998
y = absorbance
x = solute concentration (mol/L)
END OF
PAGE
2
, CHM2046L FINAL EXAM LATEST
UF SPRING 2026 - 2027
a y-intercept of 0 indicates 0 absorbance at 0 M concentration of colored
solute
Serial Dilution:
- Perform calculations to determine the amount of 6.00x10-5 M stock
solution needed to prepare 20.00 mL of 2.00x10-5 M dye solution.
- Perform calculations to determine the amount of 2.00x10-5 M stock
solution needed to prepare 20.00 mL of 1.00x10-5 M dye solution.
- Perform calculations to determine the amount of 1.00x10-5 M stock
solution needed to prepare 20.00 mL of 2.00x10-6 M dye solution. -
ANSWERS-x = 6.67 ml
x = 10 ml
x = 4 ml
END OF
PAGE
3
, CHM2046L FINAL EXAM LATEST
UF SPRING 2026 - 2027
using this Beer's Law calibration curve equation, y=22066x + 0.008, at
λmax = 420.6 nm, determine the concentrations for each of the
absorbances values below:
absorbance 1: 2.224
absorbance 2: 1.558
if absorbance 1 is from a 1e-3 M stock solution and absorbance 2 is from
a 1e-4 M stock solution, do all of these data points follow Beer's Law?
(did the concentrations you obtained using the equation match the
concentrations of the stock solutions?). If not, why? (***hint: look at the
excel graph you generated and determine the maximum absorbance at
λmax = 420.6 nm that falls on/very close to the trendline) - ANSWERS-
for absorbance 1:
x = 1e-4 M
for absorbance 2:
x = 7.02e-5 M
END OF
PAGE
4
UF SPRING 2026 - 2027
beer-lambert law - ANSWERS-A = εdc
A = absorbance
ε = molar absorptivity (L mol-1 cm-1)
d = distance the light travels through the solution (the cuvette) in cm
c = concentration of the solution (mol/L)
Beer's Law calibration curve:
Use the following data to generate a calibration curve of Absorbance vs
Concentration at λmax = 420.6 nm.
concentration 1: 6e-5 M; absorbance 1: 1.330
concentration 2: 2e-5 M; absorbance 1: 0.451
concentration 1: 1e-5 M; absorbance 1: 0.239
END OF
PAGE
1
, CHM2046L FINAL EXAM LATEST
UF SPRING 2026 - 2027
concentration 1: 2e-6 M; absorbance 1: 0.042
Record the equation and analyze your data:
- do all of the data points fall on the trendline? Is your R2 value greater
than 0.990?
- what does y represent?
- what does x represent?
- why should the y-intercept of the linear trendline be very close to 0? -
ANSWERS-equation: y=22066x + 0.008
yes, all points fall on the trendline or are very close. R2 = 0.9998
y = absorbance
x = solute concentration (mol/L)
END OF
PAGE
2
, CHM2046L FINAL EXAM LATEST
UF SPRING 2026 - 2027
a y-intercept of 0 indicates 0 absorbance at 0 M concentration of colored
solute
Serial Dilution:
- Perform calculations to determine the amount of 6.00x10-5 M stock
solution needed to prepare 20.00 mL of 2.00x10-5 M dye solution.
- Perform calculations to determine the amount of 2.00x10-5 M stock
solution needed to prepare 20.00 mL of 1.00x10-5 M dye solution.
- Perform calculations to determine the amount of 1.00x10-5 M stock
solution needed to prepare 20.00 mL of 2.00x10-6 M dye solution. -
ANSWERS-x = 6.67 ml
x = 10 ml
x = 4 ml
END OF
PAGE
3
, CHM2046L FINAL EXAM LATEST
UF SPRING 2026 - 2027
using this Beer's Law calibration curve equation, y=22066x + 0.008, at
λmax = 420.6 nm, determine the concentrations for each of the
absorbances values below:
absorbance 1: 2.224
absorbance 2: 1.558
if absorbance 1 is from a 1e-3 M stock solution and absorbance 2 is from
a 1e-4 M stock solution, do all of these data points follow Beer's Law?
(did the concentrations you obtained using the equation match the
concentrations of the stock solutions?). If not, why? (***hint: look at the
excel graph you generated and determine the maximum absorbance at
λmax = 420.6 nm that falls on/very close to the trendline) - ANSWERS-
for absorbance 1:
x = 1e-4 M
for absorbance 2:
x = 7.02e-5 M
END OF
PAGE
4
