Molecular Diagnostics Practice
Comprehensive Resource To Help You Ace 2026-2027 Exams
Includes Frequently Tested Questions With ELABORATED
100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. Which nitrogen based would bind with a guanine nucleotide in forming
double-stranded DNA?
a. Cytosine
b. Uracil
c. Thymine
d. Adenine - Correct Answer: Cytosine
2. Which probe technique is categorized as a target amplification technique?
a. Polymerase chain reaction
b. Southern blot
c. Northern blot
d. Sandwich hybridization - Correct Answer: Polymerase chain
reaction
3. The pairing or annealing of two strands of DNA is termed:
a. Probe
b. Denaturation
c. Hybridization
d. Recombination - Correct Answer: Hybridization
,4. Important applications of polymerase chain reaction (PCR) include the
following with the general exception of:
a. Search for mutations with large deletions
b. Amplification of DNA
c. Identification of a target sequence
d. Synthesis of an anti0sense probe - Correct Answer: Search for
mutations with large deletions
5. What is a disadvantage to molecular testing methods, such as nucleic acid
amplification?
a. Increased sensitivity and specificity
b. Rapid detection or organisms
c. Use of direct clinical sample
d. Specialized detection equipment - Correct Answer: Specialized
detection equipment
6. All of the following are enzymes used in molecular amplification testing
methods, EXCEPT?
a. RNase
b. Reverse transcriptase
c. RNA polymerase
d. Amylase - Correct Answer: Amylase
7. A nucleic acid used to identify a hybridization target is termed:
a. Probe
b. Oligonucleotide
c. Amplicon
d. DNA primer - Correct Answer: Probe
, 8. What molecular technique is appropriate for HIV-1 genotyping?
a. Reverse Transcriptase Polymerase Chain Reaction
b. Multiplex Polymerase Chain Reaction
c. Real-Time Polymerase Chain Reaction
d. Strand Displacement Amplification - Correct Answer: Reverse
Transcriptase Polymerase Chain Reaction
9. In a PCR amplification method, which of the following is amplified?
a. Target
b. Signal
c. Primer
d. Enzyme - Correct Answer: Target
10.The traditional Polymerase Chain Reaction (PCR) procedure has three
important functions and include all of the following EXCEPT:
a. Synthesis of a labeled antisense probe
b. Identification of a target nucleotide sequence
c. Amplifies DNA
d. Amplifies RNA - Correct Answer: Amplifies RNA
11.The following steps were used in a procedure to identify M. kansasii:
1 - Extraction
2 - Denaturization
3 - Hybridization
4 - Hydrolysis of excess reagent
5 - Detection of acridinium ester
12.Which of the following methods has been described?
Comprehensive Resource To Help You Ace 2026-2027 Exams
Includes Frequently Tested Questions With ELABORATED
100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. Which nitrogen based would bind with a guanine nucleotide in forming
double-stranded DNA?
a. Cytosine
b. Uracil
c. Thymine
d. Adenine - Correct Answer: Cytosine
2. Which probe technique is categorized as a target amplification technique?
a. Polymerase chain reaction
b. Southern blot
c. Northern blot
d. Sandwich hybridization - Correct Answer: Polymerase chain
reaction
3. The pairing or annealing of two strands of DNA is termed:
a. Probe
b. Denaturation
c. Hybridization
d. Recombination - Correct Answer: Hybridization
,4. Important applications of polymerase chain reaction (PCR) include the
following with the general exception of:
a. Search for mutations with large deletions
b. Amplification of DNA
c. Identification of a target sequence
d. Synthesis of an anti0sense probe - Correct Answer: Search for
mutations with large deletions
5. What is a disadvantage to molecular testing methods, such as nucleic acid
amplification?
a. Increased sensitivity and specificity
b. Rapid detection or organisms
c. Use of direct clinical sample
d. Specialized detection equipment - Correct Answer: Specialized
detection equipment
6. All of the following are enzymes used in molecular amplification testing
methods, EXCEPT?
a. RNase
b. Reverse transcriptase
c. RNA polymerase
d. Amylase - Correct Answer: Amylase
7. A nucleic acid used to identify a hybridization target is termed:
a. Probe
b. Oligonucleotide
c. Amplicon
d. DNA primer - Correct Answer: Probe
, 8. What molecular technique is appropriate for HIV-1 genotyping?
a. Reverse Transcriptase Polymerase Chain Reaction
b. Multiplex Polymerase Chain Reaction
c. Real-Time Polymerase Chain Reaction
d. Strand Displacement Amplification - Correct Answer: Reverse
Transcriptase Polymerase Chain Reaction
9. In a PCR amplification method, which of the following is amplified?
a. Target
b. Signal
c. Primer
d. Enzyme - Correct Answer: Target
10.The traditional Polymerase Chain Reaction (PCR) procedure has three
important functions and include all of the following EXCEPT:
a. Synthesis of a labeled antisense probe
b. Identification of a target nucleotide sequence
c. Amplifies DNA
d. Amplifies RNA - Correct Answer: Amplifies RNA
11.The following steps were used in a procedure to identify M. kansasii:
1 - Extraction
2 - Denaturization
3 - Hybridization
4 - Hydrolysis of excess reagent
5 - Detection of acridinium ester
12.Which of the following methods has been described?
