Molecular Diagnostics Study
Comprehensive Resource To Help You Ace 2026-2027 Exams
Includes Frequently Tested Questions With ELABORATED
100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. List advantages of molecular diagnostics
- Correct Answer: - increased sens and spec
- faster TAT
- genetic diagnosis identifies the mechanism responsible for the phenotype
2. List disadvantages of molecular diagnostics
- Correct Answer: - detection of pathogen DNA/RNA does not guarantee active
colonization
- false neg as a result of mutations at the primer/probe binding sites
- potential for contamination
3. What are the nanodrop measurements for nucleic acids?
- Correct Answer: 260 nm
4. What are the nanodrop measurements for proteins?
- Correct Answer: 280 nm
5. What indicates pure DNA on the nanodrop?
- Correct Answer: 1.8 (lower is less pure)
6. What indicates pure RNA on the nanodrop?
- Correct Answer: 2.0 (lower is less pure)
7. Why is messenger RNA isolated when analyzing gene expression?
, - Correct Answer: mature mRNA has a poly A tail (DNA and RNA do NOT).
extraction can have poly T linkers to beads or columns to selectively bind to the
mRNA. other RNA gets washed away and leaves us with purified mRNA.
8. What is the purpose of gel electrophoresis?
- Correct Answer: separate DNA by size
9. What is the charge of nucleic acids?
- Correct Answer: nucleic acids are negatively charged, so during gel
electrophoresis they "run to red" (negative to positive electrode)
10. True or false?
larger fragments travel further than smaller fragments.
- Correct Answer: false, smaller fragments travel farther
11. What types of stains are used during or after gel electrophoresis to detect
bands of nucleic acids or proteins?
- Correct Answer: - ethidium bromide: for dsDNA
- SYBR green: ssDNA, dsDNA or RNA
- silver stain: more sens for all of the above
12. which stain can be visible with UV light? which stain requires specific filters?
- Correct Answer: ethidium bromide can be viewed with UV light. SYBR green
requires specific filters.
13. what does band intensity equate to during gel electrophoresis?
- Correct Answer: DNA quantity
14. accuracy
- Correct Answer: correct result
- closeness of agreement between measured value and value that is accepted
- requires GOLD standard or ACCEPTED method
Comprehensive Resource To Help You Ace 2026-2027 Exams
Includes Frequently Tested Questions With ELABORATED
100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. List advantages of molecular diagnostics
- Correct Answer: - increased sens and spec
- faster TAT
- genetic diagnosis identifies the mechanism responsible for the phenotype
2. List disadvantages of molecular diagnostics
- Correct Answer: - detection of pathogen DNA/RNA does not guarantee active
colonization
- false neg as a result of mutations at the primer/probe binding sites
- potential for contamination
3. What are the nanodrop measurements for nucleic acids?
- Correct Answer: 260 nm
4. What are the nanodrop measurements for proteins?
- Correct Answer: 280 nm
5. What indicates pure DNA on the nanodrop?
- Correct Answer: 1.8 (lower is less pure)
6. What indicates pure RNA on the nanodrop?
- Correct Answer: 2.0 (lower is less pure)
7. Why is messenger RNA isolated when analyzing gene expression?
, - Correct Answer: mature mRNA has a poly A tail (DNA and RNA do NOT).
extraction can have poly T linkers to beads or columns to selectively bind to the
mRNA. other RNA gets washed away and leaves us with purified mRNA.
8. What is the purpose of gel electrophoresis?
- Correct Answer: separate DNA by size
9. What is the charge of nucleic acids?
- Correct Answer: nucleic acids are negatively charged, so during gel
electrophoresis they "run to red" (negative to positive electrode)
10. True or false?
larger fragments travel further than smaller fragments.
- Correct Answer: false, smaller fragments travel farther
11. What types of stains are used during or after gel electrophoresis to detect
bands of nucleic acids or proteins?
- Correct Answer: - ethidium bromide: for dsDNA
- SYBR green: ssDNA, dsDNA or RNA
- silver stain: more sens for all of the above
12. which stain can be visible with UV light? which stain requires specific filters?
- Correct Answer: ethidium bromide can be viewed with UV light. SYBR green
requires specific filters.
13. what does band intensity equate to during gel electrophoresis?
- Correct Answer: DNA quantity
14. accuracy
- Correct Answer: correct result
- closeness of agreement between measured value and value that is accepted
- requires GOLD standard or ACCEPTED method
