BioChem 210 Final EXAM QUESTIONS
AND CORRECT ANSWERS (VERIFIED
ANSWERS) PLUS RATIONALES 2026 Q&A
| INSTANT DOWNLOAD PDF
1. Enzyme Kinetics
Which parameter reflects enzyme affinity for substrate?
A. Vmax
B. Km
C. kcat
D. pH
Answer: B. Km
Rationale: In Michaelis-Menten kinetics, Km represents the substrate concentration at half
Vmax; lower Km indicates higher enzyme-substrate affinity.
2. Enzyme Inhibition
Competitive inhibitors:
A. Decrease Vmax
B. Increase Km
C. Bind irreversibly
D. Lower substrate binding
Answer: B. Increase Km
Rationale: Competitive inhibitors compete with substrate for the active site, increasing Km while
Vmax remains unchanged.
3. Protein Structure
Which level involves peptide bonds?
,A. Primary
B. Secondary
C. Tertiary
D. Quaternary
Answer: A. Primary
Rationale: Primary structure is the linear sequence of amino acids linked by peptide bonds.
4. Amino Acids
Which is acidic?
A. Lysine
B. Arginine
C. Aspartate
D. Valine
Answer: C. Aspartate
Rationale: Aspartate contains a carboxyl group that donates protons, making it acidic.
5. Buffers
Buffers work best when pH is near:
A. 0
B. 7
C. pKa
D. 14
Answer: C. pKa
Rationale: Buffers are most effective when pH ≈ pKa, allowing them to resist changes in
hydrogen ion concentration.
6. Metabolism
Which process produces NADH?
A. Glycolysis
B. Translation
, C. DNA replication
D. Transcription
Answer: A. Glycolysis
Rationale: Glycolysis generates NADH during oxidation of glyceraldehyde-3-phosphate.
7. ATP Yield
Total ATP per glucose (approximate)?
A. 2
B. 4
C. 30–32
D. 100
Answer: C. 30–32
Rationale: Complete aerobic respiration yields about 30–32 ATP via pathways including
Oxidative phosphorylation.
8. Citric Acid Cycle
Produces:
A. Glucose
B. CO₂
C. Oxygen
D. Lactate
Answer: B. CO₂
Rationale: The Citric Acid Cycle releases CO₂ as a waste product while generating NADH and
FADH₂.
9. DNA Replication
DNA polymerase works:
A. 3’→5’
B. 5’→3’
C. Both directions
D. Randomly
AND CORRECT ANSWERS (VERIFIED
ANSWERS) PLUS RATIONALES 2026 Q&A
| INSTANT DOWNLOAD PDF
1. Enzyme Kinetics
Which parameter reflects enzyme affinity for substrate?
A. Vmax
B. Km
C. kcat
D. pH
Answer: B. Km
Rationale: In Michaelis-Menten kinetics, Km represents the substrate concentration at half
Vmax; lower Km indicates higher enzyme-substrate affinity.
2. Enzyme Inhibition
Competitive inhibitors:
A. Decrease Vmax
B. Increase Km
C. Bind irreversibly
D. Lower substrate binding
Answer: B. Increase Km
Rationale: Competitive inhibitors compete with substrate for the active site, increasing Km while
Vmax remains unchanged.
3. Protein Structure
Which level involves peptide bonds?
,A. Primary
B. Secondary
C. Tertiary
D. Quaternary
Answer: A. Primary
Rationale: Primary structure is the linear sequence of amino acids linked by peptide bonds.
4. Amino Acids
Which is acidic?
A. Lysine
B. Arginine
C. Aspartate
D. Valine
Answer: C. Aspartate
Rationale: Aspartate contains a carboxyl group that donates protons, making it acidic.
5. Buffers
Buffers work best when pH is near:
A. 0
B. 7
C. pKa
D. 14
Answer: C. pKa
Rationale: Buffers are most effective when pH ≈ pKa, allowing them to resist changes in
hydrogen ion concentration.
6. Metabolism
Which process produces NADH?
A. Glycolysis
B. Translation
, C. DNA replication
D. Transcription
Answer: A. Glycolysis
Rationale: Glycolysis generates NADH during oxidation of glyceraldehyde-3-phosphate.
7. ATP Yield
Total ATP per glucose (approximate)?
A. 2
B. 4
C. 30–32
D. 100
Answer: C. 30–32
Rationale: Complete aerobic respiration yields about 30–32 ATP via pathways including
Oxidative phosphorylation.
8. Citric Acid Cycle
Produces:
A. Glucose
B. CO₂
C. Oxygen
D. Lactate
Answer: B. CO₂
Rationale: The Citric Acid Cycle releases CO₂ as a waste product while generating NADH and
FADH₂.
9. DNA Replication
DNA polymerase works:
A. 3’→5’
B. 5’→3’
C. Both directions
D. Randomly
