Histotechnology Exam 1 Questions
(chapter 1) and Answers Updated
2026
WhatAmethodAisAusedAtoAstabilizeAproteinsAmakingAitAresistantAtoAfurtherAdamage?A-
AAnswer✓AFixation
NameAtwoAmethodsAofAfixation?A-
AAnswer✓AChemicalAreactionA(formaldehyde)AandAphysicalA(heatAorAdessication)
WhatAareAtwoAcausesAofAPostAmortemAputrefactionAifAtheAtissueAisAnotAfixed?A*****A-
AAnswer✓AbacteriaAorAmold
WhatAfactorAcausesAautolysisAifAtissueAisAnotAfixed?A****A-AAnswer✓AenzymeAattack
WhatAareAfourAfunctionsAofAfixatives?A-
AAnswer✓A1.AkillsAtissueAtoApreventAputrefactionAandAautolysis
2.AmaintainsAproperArelationshipAbetweenAintracellularAandAextracellularAsubstancesA
3.ApreservesArefractiveAindexes
4.AenhancesAstains
WhatAthreeAorgansAcontainAtheAhighestAlevelsAofAenzymes?A-
AAnswer✓Aliver,Akidney,AandAbrain
TrueAorAFalse.AYouAcanAoverAfixAtissue?A-
AAnswer✓ATrue.AOverAfixationAdriesAoutAtheAtissueAandAlimitsAtheAquality.ACanAattemptA
steamingAtheAsectionsAtoAremedyAoverAfixation.
IfAyouAreceiveAaAbreastAsampleAonAaAFridayAnight,AwhatAmethodAmustAbeAperformedAtoApr
eventAexceedingAtheAallowedAformalinAfixationAtimeAtoAperformAtheAHer2Atest?A-
AAnswer✓AMustAplaceAtheAtissueAinA70%AalcoholAforAoneAdayAandAthenAplaceAinAformal
inAtheAfollowingAday.
, HowAquicklyAdoesAformalinApenetrateAtissue?A-AAnswer✓A1AmmAperAhour
TrueAorAFalse.AWhenAfixingAaAliverAspecimen,AtheAfixationAtimeAshouldAbeAdoubled?A-
AAnswer✓ATrue.ATheAenzymesAfoundAinAtheAcytoplasmAneedAtoAbeAfixedAwell.
WhatAisAtheAnameAofAaAfixativeAthatAisAcombinedAwithAtheAproteinAmolecule?AGiveAanAexa
mple.A-AAnswer✓AadditiveAfixativeA
Ex.Aformaldehyde
WhatAisAtheAnameAofAaAfixativeAthatAremovesAwaterAfromAtissue?AGiveAtwoAexamplesA-
AAnswer✓AnonadditiveAfixative.A
Ex.AalcoholAorAacetone
AtAwhatApHAdoesAformaldehydeAcross-linkAtheAaminoAgroups?A-AAnswer✓A7.0
NameAthreeAfactorsAthatAinfluenceAfixation?A-AAnswer✓Atemperature,Asize,AandAtime
AtAwhatAtemperatureAareAmostAfixativesAperformedAat?A-
AAnswer✓AroomAtemperatureA(20°C)AupAtoA45°C
WhatAsizeAshouldAsectionsAofAtissueAbeAsampledAthatAareAbeingAprocessedAforAroutineAproc
essingA(10-12Ahours)?A-AAnswer✓A3mmAthickA(thinnerAforAshorterAprocessingAtimes)
WhatAsizeAshouldAsectionsAofAtissueAbeAsampledAthatAareAbeingAprocessedAforAwholeAmoun
tsA(36AhourAprocessing)?A-AAnswer✓A8mmAthick
WhatAeventAmustAoccurAforApenetrationAofAfixativeAtoAoccur?A-
AAnswer✓ABloodAsupplyAmustAstop
WhatAwillAbeApresentAinAtheAcenterAofAtissueAthatAhasAaAlegoAappearance?AWhatAstageAw
asAinterruptedAtoAcauseAthisAtoAhappen?A-
AAnswer✓AwaterAwillAbeApresentAandAtheAcenterAwillAnotAbeAproperlyAfixed.AAlcoholAwa
sAnotAableAtoAproperlyApenetrateAtheAtissue.AAlcoholAremovesAwaterAfromAtissue.
(chapter 1) and Answers Updated
2026
WhatAmethodAisAusedAtoAstabilizeAproteinsAmakingAitAresistantAtoAfurtherAdamage?A-
AAnswer✓AFixation
NameAtwoAmethodsAofAfixation?A-
AAnswer✓AChemicalAreactionA(formaldehyde)AandAphysicalA(heatAorAdessication)
WhatAareAtwoAcausesAofAPostAmortemAputrefactionAifAtheAtissueAisAnotAfixed?A*****A-
AAnswer✓AbacteriaAorAmold
WhatAfactorAcausesAautolysisAifAtissueAisAnotAfixed?A****A-AAnswer✓AenzymeAattack
WhatAareAfourAfunctionsAofAfixatives?A-
AAnswer✓A1.AkillsAtissueAtoApreventAputrefactionAandAautolysis
2.AmaintainsAproperArelationshipAbetweenAintracellularAandAextracellularAsubstancesA
3.ApreservesArefractiveAindexes
4.AenhancesAstains
WhatAthreeAorgansAcontainAtheAhighestAlevelsAofAenzymes?A-
AAnswer✓Aliver,Akidney,AandAbrain
TrueAorAFalse.AYouAcanAoverAfixAtissue?A-
AAnswer✓ATrue.AOverAfixationAdriesAoutAtheAtissueAandAlimitsAtheAquality.ACanAattemptA
steamingAtheAsectionsAtoAremedyAoverAfixation.
IfAyouAreceiveAaAbreastAsampleAonAaAFridayAnight,AwhatAmethodAmustAbeAperformedAtoApr
eventAexceedingAtheAallowedAformalinAfixationAtimeAtoAperformAtheAHer2Atest?A-
AAnswer✓AMustAplaceAtheAtissueAinA70%AalcoholAforAoneAdayAandAthenAplaceAinAformal
inAtheAfollowingAday.
, HowAquicklyAdoesAformalinApenetrateAtissue?A-AAnswer✓A1AmmAperAhour
TrueAorAFalse.AWhenAfixingAaAliverAspecimen,AtheAfixationAtimeAshouldAbeAdoubled?A-
AAnswer✓ATrue.ATheAenzymesAfoundAinAtheAcytoplasmAneedAtoAbeAfixedAwell.
WhatAisAtheAnameAofAaAfixativeAthatAisAcombinedAwithAtheAproteinAmolecule?AGiveAanAexa
mple.A-AAnswer✓AadditiveAfixativeA
Ex.Aformaldehyde
WhatAisAtheAnameAofAaAfixativeAthatAremovesAwaterAfromAtissue?AGiveAtwoAexamplesA-
AAnswer✓AnonadditiveAfixative.A
Ex.AalcoholAorAacetone
AtAwhatApHAdoesAformaldehydeAcross-linkAtheAaminoAgroups?A-AAnswer✓A7.0
NameAthreeAfactorsAthatAinfluenceAfixation?A-AAnswer✓Atemperature,Asize,AandAtime
AtAwhatAtemperatureAareAmostAfixativesAperformedAat?A-
AAnswer✓AroomAtemperatureA(20°C)AupAtoA45°C
WhatAsizeAshouldAsectionsAofAtissueAbeAsampledAthatAareAbeingAprocessedAforAroutineAproc
essingA(10-12Ahours)?A-AAnswer✓A3mmAthickA(thinnerAforAshorterAprocessingAtimes)
WhatAsizeAshouldAsectionsAofAtissueAbeAsampledAthatAareAbeingAprocessedAforAwholeAmoun
tsA(36AhourAprocessing)?A-AAnswer✓A8mmAthick
WhatAeventAmustAoccurAforApenetrationAofAfixativeAtoAoccur?A-
AAnswer✓ABloodAsupplyAmustAstop
WhatAwillAbeApresentAinAtheAcenterAofAtissueAthatAhasAaAlegoAappearance?AWhatAstageAw
asAinterruptedAtoAcauseAthisAtoAhappen?A-
AAnswer✓AwaterAwillAbeApresentAandAtheAcenterAwillAnotAbeAproperlyAfixed.AAlcoholAwa
sAnotAableAtoAproperlyApenetrateAtheAtissue.AAlcoholAremovesAwaterAfromAtissue.
