MLS 2212: Exam 3-part 1 Final Test
Questions with Complete Solutions
Graded A+.
This type of GNR occurs 70-80% of the time - Answer Enteric GNR
Which two groups of GNR's occur in the lab 10-15% of the time? - Answer 1) Non-
fermentative GNR
2) Fastidious GNR
Which GNR group shows up less than 1% of the time in clinical lab specimens? - Answer
Misc. Infrequently encountered GNR
What is Enteric GNR's family? - Answer Enterobacteriaceae
Name the genera that fall under Enterobacteriaceae - Answer Escherichia
Edwardsiella
Enterobacter
Citrobacter
Klebsiella
Proteaus
Morganella
Providencia
Serratia
Salmonella
Shigella
Yersinia
Name the 3 categories Enteric GNRs fall under - Answer 1) opportunistic
2) intestinal pathogens
3) non-intestinal pathogens
Enteric GNR's are _____________ pathogens. Give 5 examples of how they would use this
"power" - Answer opportunistic
1) UTI's
,2) Wounds
3) septicemia
4) Lower respiratory tract infections
5) meningitis
Enteric GNR's that are found in the intestinal tract that would NOT be considered normal flora
include
*** these 3 are always significant when found in a clinical specimen, because of this where
would you report these out to? - Answer 1) salmonella
2) E.coli (the ones that causes diarrheal illnesses)
3) Yersinia enterocolitica
You would report these specimens to the state lab.
Which Enteric GNR is considered a "non-intestinal pathogen" - Answer Yersinia pestis
(bubonic or black plague)
What are the 3 ways that MLS personnel can identifiy enteric GNR'S - Answer 1) Manual
2) Automated instruments
3) Some are referred to state health lab for serological testing
Tell me what is included in the Manuel approach when identifying Enteric GNR - Answer
Gram stain, selective & differential plating media, biochemical tests (spots tests and tubed
media)
* profile 3-4 key test
Tell me what is included in the automated instruments and ID kits when identifying Enteric
GNR'S. Give two examples of instruments - Answer 20-30 biochemical tests, involves large
data bases for a statistical match
1) microscan "walkaway" has a panel in a well card
2) vitek uses a card
3) API strips (analytical profile index)
What are the 4 common characteristics that are in enteric GNR's - Answer 1) facultative
anaerobes (can grow in aerobic or anaerobic)
2) non-fastidious (not picky)
, 3) most are normal in human fecal flora (can be seen in small and large intestine)
4) Ubiquitous in nature - found everywhere (except for Intentional pathogens)
GNR's that are commonly seen in the small and large intestine are called _____________ or
_______________. Also what is their function in the stomach? Give an example of a organism
that would be super dangerous in the stomach if it wasnt for this NF. - Answer Enterics or
Coliforms
1) Acid in breakdown of food
2) prevent pathogens from colonizing - stops opportunistic pathogens (because they take up so
much room). For example, Clostridium difficle would be extremely harmful without enterics in
the GI.
Name the 6 laboratory characteristics that enteric GNRs have in common. - Answer 1) All
reduce nitrate to nitrites (example UA dipstick)
2) oxidase negative (filter paper test)
3) All ferment glucose (ex will turn butt of TSI to yellow)
****** both highlighted in yellow are used to rule out non-enteric GNR's
4) Gram stain : Gram neg pleomorphic rods (never report out celluar arrangement with rods)
5) Colony morphology : SBA - "large gray mucoids" (larger than cocci's) Hemolysis - variable,
CHOC - "Large gray mucoid". CNA = No growth @ 48 hrs
6) Divided into lac pos and lac neg
Look at MAC chart on pg 3A- 3 - Answer (:
All Enteric GNR oxidase _____________. and there TSI are usually - Answer negative
(*/A)
MOST non-fermenative GNR are oxidase ___________. and there TSI are usually - Answer
positive
(k/k)
would you report out diplos or bipolar staining on GNR's - Answer no
How would you report out Klebsiella pnemoniae on a SBA plate? - Answer "Large gray VERY
mucoid"
If your SBA plate has the same organisms as your CHOC plate then we call in - Answer NAO
(No additional organism)
Questions with Complete Solutions
Graded A+.
This type of GNR occurs 70-80% of the time - Answer Enteric GNR
Which two groups of GNR's occur in the lab 10-15% of the time? - Answer 1) Non-
fermentative GNR
2) Fastidious GNR
Which GNR group shows up less than 1% of the time in clinical lab specimens? - Answer
Misc. Infrequently encountered GNR
What is Enteric GNR's family? - Answer Enterobacteriaceae
Name the genera that fall under Enterobacteriaceae - Answer Escherichia
Edwardsiella
Enterobacter
Citrobacter
Klebsiella
Proteaus
Morganella
Providencia
Serratia
Salmonella
Shigella
Yersinia
Name the 3 categories Enteric GNRs fall under - Answer 1) opportunistic
2) intestinal pathogens
3) non-intestinal pathogens
Enteric GNR's are _____________ pathogens. Give 5 examples of how they would use this
"power" - Answer opportunistic
1) UTI's
,2) Wounds
3) septicemia
4) Lower respiratory tract infections
5) meningitis
Enteric GNR's that are found in the intestinal tract that would NOT be considered normal flora
include
*** these 3 are always significant when found in a clinical specimen, because of this where
would you report these out to? - Answer 1) salmonella
2) E.coli (the ones that causes diarrheal illnesses)
3) Yersinia enterocolitica
You would report these specimens to the state lab.
Which Enteric GNR is considered a "non-intestinal pathogen" - Answer Yersinia pestis
(bubonic or black plague)
What are the 3 ways that MLS personnel can identifiy enteric GNR'S - Answer 1) Manual
2) Automated instruments
3) Some are referred to state health lab for serological testing
Tell me what is included in the Manuel approach when identifying Enteric GNR - Answer
Gram stain, selective & differential plating media, biochemical tests (spots tests and tubed
media)
* profile 3-4 key test
Tell me what is included in the automated instruments and ID kits when identifying Enteric
GNR'S. Give two examples of instruments - Answer 20-30 biochemical tests, involves large
data bases for a statistical match
1) microscan "walkaway" has a panel in a well card
2) vitek uses a card
3) API strips (analytical profile index)
What are the 4 common characteristics that are in enteric GNR's - Answer 1) facultative
anaerobes (can grow in aerobic or anaerobic)
2) non-fastidious (not picky)
, 3) most are normal in human fecal flora (can be seen in small and large intestine)
4) Ubiquitous in nature - found everywhere (except for Intentional pathogens)
GNR's that are commonly seen in the small and large intestine are called _____________ or
_______________. Also what is their function in the stomach? Give an example of a organism
that would be super dangerous in the stomach if it wasnt for this NF. - Answer Enterics or
Coliforms
1) Acid in breakdown of food
2) prevent pathogens from colonizing - stops opportunistic pathogens (because they take up so
much room). For example, Clostridium difficle would be extremely harmful without enterics in
the GI.
Name the 6 laboratory characteristics that enteric GNRs have in common. - Answer 1) All
reduce nitrate to nitrites (example UA dipstick)
2) oxidase negative (filter paper test)
3) All ferment glucose (ex will turn butt of TSI to yellow)
****** both highlighted in yellow are used to rule out non-enteric GNR's
4) Gram stain : Gram neg pleomorphic rods (never report out celluar arrangement with rods)
5) Colony morphology : SBA - "large gray mucoids" (larger than cocci's) Hemolysis - variable,
CHOC - "Large gray mucoid". CNA = No growth @ 48 hrs
6) Divided into lac pos and lac neg
Look at MAC chart on pg 3A- 3 - Answer (:
All Enteric GNR oxidase _____________. and there TSI are usually - Answer negative
(*/A)
MOST non-fermenative GNR are oxidase ___________. and there TSI are usually - Answer
positive
(k/k)
would you report out diplos or bipolar staining on GNR's - Answer no
How would you report out Klebsiella pnemoniae on a SBA plate? - Answer "Large gray VERY
mucoid"
If your SBA plate has the same organisms as your CHOC plate then we call in - Answer NAO
(No additional organism)
