TSI EXAMS SET 2026 PRACTICE QUESTIONS AND ANSWERS FULL SOLUTION VIEW AHEAD

TSI EXAMS SET 2026 PRACTICE QUESTIONS
AND ANSWERS FULL SOLUTION VIEW
AHEAD

●● What is a TSI (Triple Sugar Iron) Test also referred to as?
Answer: Kligers Iron Test


●● What does a TSI Test, test for?
Answer: Bacteria's ability to:
-Ferment glucose and lactose/sucrose
-Produce gas
-Reduce Sulfur


●● What are the ingredients of this media?
Answer: Glucose, Sucrose, and Lactose


●● What is the ratio of Glucose, Sucrose, and Lactose in this media?
Answer: 1:10:10


●● What is the sulfur source in a TSI Test?
Answer: Sodium Thiosulfate

, ●● What is the fermentation indicator in a TSI Test?
Answer: Phenol Red


●● What is the hydrogen sulfide indicator in a TSI Test?
Answer: Ferrous Sulfate


●● What are the 2 steps to inoculating this media?
Answer: 1.) Stab the butt
2.) Fishtail the slant


●● In the Fermentation of sugars portion of a TSI test, which sugar is
limited, and why?
Answer: Glucose, because it is bacteria's primary source of carbon


●● Which sugar will bacteria always try to ferment first?
Answer: Glucose


●● If ONLY glucose is fermented, what color will the slant and butt be?
Answer: Red slant, Yellow butt


●● If glucose is fermented, as well as lactose and/or sucrose, what color
will the slant and butt be?