AALAS LAT Certification Practice Exam (100 Questions
with Answers)
Husbandry & Environment (1–20)
1. What is the recommended temperature range for a standard
conventional rodent room?
Answer: 64–79°F (18–26°C)
2. What relative humidity range is recommended by the Guide for the
Care and Use of Laboratory Animals?
Answer: 30–70%
3. How many air changes per hour (ACH) are typically recommended for
animal rooms in a conventional facility?
Answer: 10–15 ACH
4. What light cycle is standard for rodent housing?
Answer: 12:12 hour light-dark cycle
5. What is the maximum sound level recommended in animal housing
areas?
Answer: 85 dBA (with avoidance of sudden loud noises)
6. What bedding type is most commonly used for rodent cages?
Answer: Autoclaved corncob or aspen shavings
7. What is the purpose of microisolator cages?
Answer: Reduce cross-contamination between cages
8. How often should sentinel animals be tested for pathogens in a
rodent colony?
Answer: Every 3–6 months
, 9. What is the recommended cage change frequency for a standard
mouse cage with 4–5 adult mice?
Answer: Once weekly
10. What environmental enrichment is appropriate for mice?
Answer: Nesting material (e.g., cotton squares, paper strips)
11. What is the primary purpose of an autoclave in an animal facility?
Answer: Sterilize cages, bedding, water, and equipment
12. Which chemical disinfectant is effective against parvoviruses in
rodent facilities?
Answer: Bleach (sodium hypochlorite) 1:10 dilution
13. What is the correct order of entry for a clean animal room?
Answer: PPE donning → enter clean side → husbandry → dirty side
→ decon exit
14. What is the purpose of a change station (biobubble, laminar flow
hood)?
Answer: Aseptic cage changing for immunocompromised or barrier-
housed animals
15. Which water delivery system prevents contamination in SPF rodent
colonies?
Answer: Automatic watering with sipper tubes or autoclaved water
bottles
16. What bedding disposal method is most common after infectious
studies?
Answer: Autoclaving then incineration
with Answers)
Husbandry & Environment (1–20)
1. What is the recommended temperature range for a standard
conventional rodent room?
Answer: 64–79°F (18–26°C)
2. What relative humidity range is recommended by the Guide for the
Care and Use of Laboratory Animals?
Answer: 30–70%
3. How many air changes per hour (ACH) are typically recommended for
animal rooms in a conventional facility?
Answer: 10–15 ACH
4. What light cycle is standard for rodent housing?
Answer: 12:12 hour light-dark cycle
5. What is the maximum sound level recommended in animal housing
areas?
Answer: 85 dBA (with avoidance of sudden loud noises)
6. What bedding type is most commonly used for rodent cages?
Answer: Autoclaved corncob or aspen shavings
7. What is the purpose of microisolator cages?
Answer: Reduce cross-contamination between cages
8. How often should sentinel animals be tested for pathogens in a
rodent colony?
Answer: Every 3–6 months
, 9. What is the recommended cage change frequency for a standard
mouse cage with 4–5 adult mice?
Answer: Once weekly
10. What environmental enrichment is appropriate for mice?
Answer: Nesting material (e.g., cotton squares, paper strips)
11. What is the primary purpose of an autoclave in an animal facility?
Answer: Sterilize cages, bedding, water, and equipment
12. Which chemical disinfectant is effective against parvoviruses in
rodent facilities?
Answer: Bleach (sodium hypochlorite) 1:10 dilution
13. What is the correct order of entry for a clean animal room?
Answer: PPE donning → enter clean side → husbandry → dirty side
→ decon exit
14. What is the purpose of a change station (biobubble, laminar flow
hood)?
Answer: Aseptic cage changing for immunocompromised or barrier-
housed animals
15. Which water delivery system prevents contamination in SPF rodent
colonies?
Answer: Automatic watering with sipper tubes or autoclaved water
bottles
16. What bedding disposal method is most common after infectious
studies?
Answer: Autoclaving then incineration
