WGU BIOCHEMISTRY C785 - MODULE 1 EXAM
QUESTIONS AND CORRECT ANSWERS
Anticodon - correct answer- The three bases of the
mRNA codon are complementary (i.e., they base-pair) to three
bases on the tRNA known as the anticodon
Antiparallel - correct answer- ~ The opposite
arrangement of the sugar-phosphate backbones in a DNA
double helix.
~ complementary sequence
~ Whenever 2 strands base pair, they are antiparallel to one
another.
~ RNA is always antiparallel to the template strand
Base Excision Repair (BER) - correct answer- DNA
damage repair mechanism where a single nucleotide is replaced,
1. DNA repair enzymes recognize and remove the damaged base
2. DNA pol adds a new nucleotide
,3. ligase seals the remaining nick
Briefly explain the steps in a PCR cycle. - correct answer-
1.) Need template DNA strand. DNA strands are separated by
heat. (Denaturation - heated to 95C)
2.) Primers anneal to DNA strands. (Annealing - the reaction is
cooled to 50C)
3.) Extension of newly formed DNA by DNA Polymerase.
(Elongation - DNA pol works well at 70C, so the reaction is
heated to this temp - this enables DNA pol to add nucleotides
and allows building new strands of DNA.)
4.) Cycle repeats.
#1 TECHIQUE
make primers that flank the mutation and sequence the product
#2 TECHIQUE
, use primers that stick to the mutation
- WILL HAVE LOW AMOUNT OF DNA/PRODUCT MADE, IF
MUTATION IS NOT PRESENT
Central dogma of molecular biology - correct answer-
DNA -> RNA -> Protein
a portion of the DNA, a gene, is transcribed to produce a
complementary strand of RNA; then the RNA is translated into
protein .
-The understanding that DNA is used to make RNA and RNA is
used to make protein
coding DNA strand (non template) is the same as _______ -
correct answer- mRNA strand
Coding strand DNA and RNA - correct answer- Are not
complementary
Not antiparrell
Just switch T with U in RNA
QUESTIONS AND CORRECT ANSWERS
Anticodon - correct answer- The three bases of the
mRNA codon are complementary (i.e., they base-pair) to three
bases on the tRNA known as the anticodon
Antiparallel - correct answer- ~ The opposite
arrangement of the sugar-phosphate backbones in a DNA
double helix.
~ complementary sequence
~ Whenever 2 strands base pair, they are antiparallel to one
another.
~ RNA is always antiparallel to the template strand
Base Excision Repair (BER) - correct answer- DNA
damage repair mechanism where a single nucleotide is replaced,
1. DNA repair enzymes recognize and remove the damaged base
2. DNA pol adds a new nucleotide
,3. ligase seals the remaining nick
Briefly explain the steps in a PCR cycle. - correct answer-
1.) Need template DNA strand. DNA strands are separated by
heat. (Denaturation - heated to 95C)
2.) Primers anneal to DNA strands. (Annealing - the reaction is
cooled to 50C)
3.) Extension of newly formed DNA by DNA Polymerase.
(Elongation - DNA pol works well at 70C, so the reaction is
heated to this temp - this enables DNA pol to add nucleotides
and allows building new strands of DNA.)
4.) Cycle repeats.
#1 TECHIQUE
make primers that flank the mutation and sequence the product
#2 TECHIQUE
, use primers that stick to the mutation
- WILL HAVE LOW AMOUNT OF DNA/PRODUCT MADE, IF
MUTATION IS NOT PRESENT
Central dogma of molecular biology - correct answer-
DNA -> RNA -> Protein
a portion of the DNA, a gene, is transcribed to produce a
complementary strand of RNA; then the RNA is translated into
protein .
-The understanding that DNA is used to make RNA and RNA is
used to make protein
coding DNA strand (non template) is the same as _______ -
correct answer- mRNA strand
Coding strand DNA and RNA - correct answer- Are not
complementary
Not antiparrell
Just switch T with U in RNA
