MOLECULAR DIAGNOSTICS 3RD EDITION
BUCKINGHAM TEST BANK
COMPREHENSIVE SCRIPT 2026 COMPLETE
QUESTIONS AND SOLUTIONS GRADED A+
⩥ What is the basis for paternity testing and specimen identity? Answer:
restriction enzyme fragment analysis
⩥ What is the advantage of blunt ends over sticky ends? Answer: blunt
ends can be joined together and do not have to match while sticky ends
must match exactly in order to hybridize or converted to blunt ends
through exonuclease activity or polymerase activity
⩥ CRISPR Answer: an adaptive immune system for bacteria and
archaea, clustered interspaced short palindromic repeats and case protein
which recognizes foreign DNA and keeps record of previous infections
⩥ PAM Answer: Protospacer Adjacent Motif, necessary sequence found
adjacent to the protospacer in the target DNA, discriminates target from
self
⩥ Oligonucleotides Answer: segments of nucleic acid that are 50
nucleotides or less in length
, ⩥ Why is it important in DNA isolation techniques to eliminate or
inactivate exonuclease and endonuclease activity? Answer: because
these enzymes degrade DNA
⩥ DNase I Answer: enzyme that digests DNA
⩥ Why is DNA stored in TE buffer? Answer: TE buffer chelates ions
which are needed for nuclease activity, to stabilize DNA
⩥ topoisomerase Answer: corrects "overwinding" ahead of replication
forks by interconverting isomers or relax supertwised DNA
⩥ Gyrase Answer: untangles DNA through ds breaks; separates linked
rings of DNA (type II topoisomerases)
⩥ Methyltransferase Answer: enzyme that methylates newly synthesized
DNA strands and are involved in mismatched repair
⩥ What is the difference in methylation between prokaryotes and
eukaryotes? Answer: prokaryotes use methylation to distinguish between
self and non-self, nearly all sites are methylated or hemimethyalted.
Eukaryotes use methylation in specific regions to promote or prevent
gene transcription
BUCKINGHAM TEST BANK
COMPREHENSIVE SCRIPT 2026 COMPLETE
QUESTIONS AND SOLUTIONS GRADED A+
⩥ What is the basis for paternity testing and specimen identity? Answer:
restriction enzyme fragment analysis
⩥ What is the advantage of blunt ends over sticky ends? Answer: blunt
ends can be joined together and do not have to match while sticky ends
must match exactly in order to hybridize or converted to blunt ends
through exonuclease activity or polymerase activity
⩥ CRISPR Answer: an adaptive immune system for bacteria and
archaea, clustered interspaced short palindromic repeats and case protein
which recognizes foreign DNA and keeps record of previous infections
⩥ PAM Answer: Protospacer Adjacent Motif, necessary sequence found
adjacent to the protospacer in the target DNA, discriminates target from
self
⩥ Oligonucleotides Answer: segments of nucleic acid that are 50
nucleotides or less in length
, ⩥ Why is it important in DNA isolation techniques to eliminate or
inactivate exonuclease and endonuclease activity? Answer: because
these enzymes degrade DNA
⩥ DNase I Answer: enzyme that digests DNA
⩥ Why is DNA stored in TE buffer? Answer: TE buffer chelates ions
which are needed for nuclease activity, to stabilize DNA
⩥ topoisomerase Answer: corrects "overwinding" ahead of replication
forks by interconverting isomers or relax supertwised DNA
⩥ Gyrase Answer: untangles DNA through ds breaks; separates linked
rings of DNA (type II topoisomerases)
⩥ Methyltransferase Answer: enzyme that methylates newly synthesized
DNA strands and are involved in mismatched repair
⩥ What is the difference in methylation between prokaryotes and
eukaryotes? Answer: prokaryotes use methylation to distinguish between
self and non-self, nearly all sites are methylated or hemimethyalted.
Eukaryotes use methylation in specific regions to promote or prevent
gene transcription
